Biomedical subjects

J Pio

Publications and source records attributed to J Pio.

9 recordsLinked to original sources

PubMed2001

Distribution of C-reactive protein and its relation to risk factors and coronary heart disease risk estimation in the National Health and Nutrition Examination Survey (NHANES) III.

The authors examined the distribution of, and risk factors associated with, the inflammatory marker C-reactive protein (CRP) among a large sample of non-institutionalized American adults aged 30-74 years of age, and its relation to estimated 10-year coronary heart disease risk. The population studied comprised 4472 men and 5212 women aged 30-74 years, without coronary heart disease, who had CRP measurements in the Third National Health and Nutrition Examination Survey (NHANES III). The 10-year risk of coronary heart disease was estimated from Framingham risk factor algorithms among those with CRP levels of less-than-or-equal0.21 mg/dL, >0.21 to <0.5mg/dL, 0.5 to <1.0 mg/dL, and greater-than-or-equal1.0 mg/dL. Mean (SD) levels of CRP were 0.41 (0.64) mg/dL in men and 0.55 (0.91) mg/dL in women. Levels of at least 1 mg/dL were measured in 6.4% of men and 12.9% of women. CRP levels were highest among non-Hispanic black men and Mexican-American women. According to multiple logistic regression analysis, cigarette smoking and increased age, body mass index, and systolic blood pressure in men, and body mass index and diabetes in women, were strongly associated with a greater likelihood of CRP levels of greater-than-or-equal1.0 mg/dL (p<0.001). Among persons with CRP levels of less-than-or-equal0.21 mg/dL, >0.21 to <0.5 mg/dL, 0.5 to <1.0 mg/dL and greater-than-or-equal1.0 mg/dL, the 10-year estimated risk of coronary heart disease were 13.4%, 17.6%, 19.6%, and 21.1% among men, respectively, and 2.7%, 3.6%, 4.1% and 4.3% among women, respectively (both p<0.001 across CRP categories); higher risks across CRP groups were also found among ethnic/gender subgroups. CRP remained a significant predictor of coronary heart disease risk in unadjusted and age-adjusted analyses. Conclusion. Elevation of CRP is associated with several major coronary heart disease risk factors and with unadjusted and age-adjusted projections of 10-year coronary heart disease risk in both men and women. (c)2001 CHF, Inc. Presented at the Fifth International Conference on Preventive Cardiology, Osaka, Japan, May, 2001.

Journal Article↗

PubMed1999

Postnatal development of synaptophysin immunoreactivity in the rat nucleus tractus solitarii and caudal ventrolateral medulla.

Synaptophysin (SY) is a major integral membrane protein of small synaptic vesicles. In the present study, SY immunohistochemistry was used to investigate the postnatal development of the rat nucleus tractus solitarii (NTS) and nucleus ambiguus/ventrolateral medulla (NA/VLM). Whatever the age of the animal, SY immunoreactivity showed a typical pattern of punctate staining reminiscent of presynaptic terminal labeling. In the NTS and the NA/VLM, SY immunoreactive puncta were few at birth and increased in number during the first postnatal days. These changes were quantified by measuring the volumetric fraction occupied by SY immunoreactive puncta at various postnatal ages. Using volumetric fraction data, an index of the total volume occupied SY immunoreactivity in each region was then calculated. Between birth and adulthood, this index increased by 6-fold in the NTS and by 7-fold in the NA/VLM, suggesting that most of the synaptic development of these regions occurs postnatally.

Aging↗

PubMed1997

CNS axonal regeneration with peripheral nerve grafts cryopreserved by vitrification: cytological and functional aspects.

To test cool-warm protocols for storing peripheral nerves, 4-cm-long-nerve segments were removed from the hindleg of adult rats and cryopreserved using a vitrification solution (or cryoprotective mixture) containing a mixture of polyalcohols (2,3-butanediol, 1,2-propanediol, polyethylene glycol, and Belzer U.W. medium). Schwann cell viability and morphology were studied with regard to the effect of (i) cryoprotective mixture concentration (100, 50, and 30% diluted in human serum albumin at 4%), (ii) duration of exposure (10, 15, or 30 min in a single step) of nerves to the cryoprotective mixture, (iii) cooling rate (F1/F2, F3, and F4: 3, 12, and 231 degrees C/min, respectively), and (iv) type of replacement of cryoprotectant (T1, one step; or T2, perfusion) after warming. Nerves exposed 10 min to cryoprotective mixture 50% (2,3-butanediol, 1.926 mol.liter-1; 1,2-propanediol, 3.063 mol.liter-1; polyethylene glycol, 0.084 mol.liter-1; and Belzer U.W., 22.4 mosm-1) and cooled-warmed with the F2/F3/F4-T2 protocols contained live and correctly cryopreserved Schwann cells. The capacity of these cryopreserved nerve segments (n = 6) to be subsequently repopulated by regenerating axons from central neurons was compared to that of fresh nerves when used as peripheral nerve autografts implanted within the spinal cord at the level of the descending respiratory pathways. All cryopreserved nerve grafts were successfully reinnervated by regenerated central axons. Unitary spontaneous action potentials propagated along these axons were assessed by recording the discharge of tested nervous filaments (T) from the grafts in artificially ventilated and paralyzed animals. Out of 535 T, 32 (6 +/- 1.2%) presented spontaneous unitary activity with respiratory (R, n = 2) and nonrespiratory (NR, n = 30) pattern of discharge. The T mean number, the occurrence rate referenced to the total number of T (R/T, NR/T, and R + NR/T) and the mean number of spontaneous units (R, NR, R + NR) were compared to those of fresh spinal peripheral nerve grafts. Except for T, cryopreserved peripheral nerve grafts contained statistically significantly (P < 0.05) less spontaneous R and NR unitary activity, which represented, respectively, 6.2 +/- 6.2 and 26.8 +/- 5.7% of that found in the control group. These data indicate that nerves cryopreserved with the protocols described above contain viable Schwann cells which constitute a suitable support to induce regeneration of central fibers. The effectiveness of nerve cryopreservation by vitrification is discussed with regard to Schwann cell viability following cool-warm protocols and to subsequent reinnervation of the cryopreserved peripheral nerve grafts.

Animals↗

PubMed1990

Selective retrograde labeling of primary vagal afferent cell-bodies after injection of [3H]D-aspartate into the rat nucleus tractus solitarii.

A selective retrograde labeling study was performed using [3H]D-aspartate to identify putative glutamatergic and/or aspartatergic primary vagal afferent fibers. Unilateral microinjection of [3H]D-aspartate into the nucleus tractus solitarii resulted in clear visible labeling of a fraction of the neuronal cell-bodies in the nodose ganglia. The labeled cell-bodies were randomly distributed in the ganglion and more numerous labeled neurons were detected in the ipsilateral than in the contralateral ganglion (4.93% and 0.98% of the neurons sampled within the ipsi- and contralateral ganglia, respectively). These results strongly suggest that primary vagal afferent fibers may utilize excitatory amino acids as transmitters.

Animals↗

PubMed1969