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Biomedical subjects

J Prakash

Publications and source records attributed to J Prakash.

17 recordsLinked to original sources
PubMed1992

Clinical significance of kidney biopsy in acute renal failure (ARF).

Out of 152 cases of Acute renal failure (ARF) 32 patients (21%) were subjected to kidney biopsy. All patients had intrinsic ARF. Prerenal azotemia and obstructive uropathy were excluded. Histologic observations were: Crescentric glomerulonephritis in 7 (21.9%), acute endocapillary proliferative glomerulonephritis 5 (15.6%), acute interstitial nephritis 7 (21.9%), necrotizing vasculitis 4 (12.5%), acute tubular necrosis in 5 (15.6%) and membrano-proliferative GN with superimposed crescent in 2 (6.2%) while renal cortical necrosis was seen in 6.2% of cases. Prebiopsy diagnosis was correct in only 10 (31.25%) cases. The result of biopsy had altered clinical diagnosis in 22 (68.75%) patients and precise renal biopsy diagnosis resulted in therapeutic changes in 54.8% of patients with ARF.

Acute Kidney Injury↗
PubMed1991

Upper gastrointestinal mucosal lesions in chronic renal failure.

The upper gastrointestinal mucosa was studied endoscopically in 182 patients (140 males, 42 females) with chronic renal failure prior to hemodialysis. Endoscopy revealed normal mucosa in 77 patients (42.3%), inflammatory mucosal lesions in 88 (48.4%), peptic ulcer in 16 (8.8%; duodenal 15, gastric 1) and Barrett's ulcer in one patient. Upper gastrointestinal bleeding was noted at presentation in 16 (8.8%) cases and was associated with erosive gastritis, duodenitis and duodenal ulcer in 11, 3 and 2 patients respectively. Thus patients with chronic renal failure had a high prevalence of inflammatory mucosal changes.

Adolescent↗
PubMed1990

Complications of intermittent peritoneal dialysis.

Two hundred and ninety three sessions of intermittent peritoneal dialysis were carried in 158 cases of renal failure between 1984 and 1987. The complications encountered during the procedure were grouped as mechanical in 29 (17.9%), circulatory in 26 (15%), neurologic in 21 (13.4%) and biochemical in 54 (34.1%) cases. Unexplained respiratory arrest with spontaneous recovery occurred in 2, infections in 5 and miscellaneous complications in 19 (12%) cases. Bradycardia and cardiac standstill were observed in one patient. Most of the complications were managed uneventfully. There was no mortality.

Acute Kidney Injury↗
PubMed1989

A monolithic patch-clamping amplifier with capacitive feedback.

Patch-clamping is an established method for directly measuring ionic transport through cellular membranes with sufficient resolution to observe open/close transitions of individual channel molecules. This paper describes an alternative technique for patch-clamping which uses a capacitor as the transimpedance element. This approach eliminates bandwidth and saturation limitations experienced with resistive patch-clamping amplifiers. A complete monolithic design featuring an on-chip operational amplifier, a capacitor array with gain-ranging from 30 pF down to 0.03 pF, and reset and gain ranging switches has been fabricated using 5 microns CMOS technology. It is shown that the voltage noise of the CMOS operational amplifier limits the overall noise performance, but that performance competitive with conventional instruments can be achieved over a 10 kHz bandwidth, at least for small input capacitances (less than or equal to 5 pF). Results are presented along with an analysis and comparison of noise performance using both resistive and capacitive elements.

Amplifiers, Electronic↗
PubMed1986

Expression of resistance to potato virus X in isolated potato protoplasts.

Resistance to Potato Virus X (PVX) in potato cultivars is conferred by the genes Nx and/or Nb. The Nx gene is activated by group 3 strains of PVX. When protoplasts from the cultivars King Edward containing the Nx gene and Pentland Crown containing neither resistance gene were inoculated with a group 3 strain of PVX resistance was expressed in the King Edward protoplasts. Replication of PVX occurred in King Edward protoplasts but only at a very low level as compared with the level observed in the susceptible Pentland Crown protoplasts. Similar results were obtained when protoplasts obtained from leaf discs mechanically inoculated with PVX were used. Virus levels were assayed by immunofluorescence and ELISA. Maintenance of resistance in protoplasts of King Edward following inoculation with PVX RNA was confirmed suggesting that resistance probably operates at the transcription stage in replication. These results clearly demonstrate single gene resistance in potato protoplasts inoculated with PVX.

Immunity, Innate↗
PubMed1985

Optimisation of conditions for infection of isolated potato protoplasts with potato virus X.

Conditions for the reproducible infection of potato protoplasts with PVX were determined. Successful virus infection was achieved when potato protoplasts at a concentration of 3 X 10(5) cells/ml were incubated with 20 micrograms/ml PVX and 2 micrograms/ml Poly-L-ornithine (MW 100,000) for 15 minutes at 0 degrees C. Numbers of protoplasts infected varied between 30 and 50 per cent. Preincubation of PVX with PLO for a minimum of 10 minutes was essential for good infection. The type and molecular weight of polycation used had a major effect on virus infection. PLO at 100,000 MW was the most efficient.

Cell Survival↗
PubMed1976

Lingual abscess.

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Abscess↗