Polynucleotides. XIV. Photochemical evidence for an extrahelical, solvent-accessible environment of non-complementary residues in polynucleotide helices.
Explore the source record for details and available documents.
Biomedical subjects
Publications and source records attributed to J R Fresco.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
tRNA(Leu)-Synthetase from baker's yeast has been crystallized from ammonium sulfate solution. The crystals are of orthorhombic symmetry, falling into space group [unk] or [unk] with a = 75.5 A, b = 110.7 A, and c = 124.0 A. This unit cell contains four molecules of the native (dimeric) enzyme, with eight asymmetric units, each corresponding to the enzyme subunit. Diffraction patterns obtained for the three major projections contain reflections to spacings of less than 3.5 A, indicating that this crystal form is amenable to structure analysis to atomic resolution.
Culture conditions were developed that permit efficient incorporation of [(32)P]phosphate into nucleic acids of baker's yeast. RNA sufficiently labeled for sequence determination was obtained in this way. Pure "denaturable" leucine acceptor transfer RNA (tRNA(Leu)(3)) was isolated for this purpose.
The nucleotide sequence of " denaturable"leucine acceptor transfer RNA (tRNA(Leu)(3)) from baker's yeast was determined on (32)P-labeled material. The molecule is 85 nucleotides long and can be folded into the "cloverleaf" model for secondary structure. The basis on which the sequence was deduced from the products of complete enzymatic digestion, prior to its unambiguous determination, is presented.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.