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J R Fresco

Publications and source records attributed to J R Fresco.

At least 55 records · Page 3Linked to original sources

Crystallization of tRNA Leu -synthetase from baker's yeast.

tRNA(Leu)-Synthetase from baker's yeast has been crystallized from ammonium sulfate solution. The crystals are of orthorhombic symmetry, falling into space group [unk] or [unk] with a = 75.5 A, b = 110.7 A, and c = 124.0 A. This unit cell contains four molecules of the native (dimeric) enzyme, with eight asymmetric units, each corresponding to the enzyme subunit. Diffraction patterns obtained for the three major projections contain reflections to spacings of less than 3.5 A, indicating that this crystal form is amenable to structure analysis to atomic resolution.

Acrylamides↗

Nucleotide sequence of the "denaturable" leucine transfer RNA from yeast.

The nucleotide sequence of " denaturable"leucine acceptor transfer RNA (tRNA(Leu)(3)) from baker's yeast was determined on (32)P-labeled material. The molecule is 85 nucleotides long and can be folded into the "cloverleaf" model for secondary structure. The basis on which the sequence was deduced from the products of complete enzymatic digestion, prior to its unambiguous determination, is presented.

Base Sequence↗