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Biomedical subjects

J R Stout

Publications and source records attributed to J R Stout.

At least 19 recordsLinked to original sources
PubMed2006

Effects of beta-alanine supplementation on the onset of neuromuscular fatigue and ventilatory threshold in women.

This study examined the effects of 28 days of beta-alanine supplementation on the physical working capacity at fatigue threshold (PWCFT), ventilatory threshold (VT), maximal oxygen consumption (VO2-MAX), and time-to-exhaustion (TTE) in women. Twenty-two women (age+/-SD 27.4+/-6.1 yrs) participated and were randomly assigned to either the beta-alanine (CarnoSyn) or Placebo (PL) group. Before (pre) and after (post) the supplementation period, participants performed a continuous, incremental cycle ergometry test to exhaustion to determine the PWCFT, VT, VO2-MAX, and TTE. There was a 13.9, 12.6 and 2.5% increase (p<0.05) in VT, PWCFT, and TTE, respectively, for the beta-alanine group, with no changes in the PL (p>0.05). There were no changes for VO2-MAX (p>0.05) in either group. Results of this study indicate that beta-alanine supplementation delays the onset of neuromuscular fatigue (PWCFT) and the ventilatory threshold (VT) at submaximal workloads, and increase in TTE during maximal cycle ergometry performance. However, beta-alanine supplementation did not affect maximal aerobic power (VO2-MAX). In conclusion, beta-alanine supplementation appears to improve submaximal cycle ergometry performance and TTE in young women, perhaps as a result of an increased buffering capacity due to elevated muscle carnosine concentrations.

Adult↗
PubMed2006

Effects of resistance training and protein plus amino acid supplementation on muscle anabolism, mass, and strength.

This study examined 10 wks of resistance training and the ingestion of supplemental protein and amino acids on muscle performance and markers of muscle anabolism. Nineteen untrained males were randomly assigned to supplement groups containing either 20 g protein (14 g whey and casein protein, 6 g free amino acids) or 20 g dextrose placebo ingested 1 h before and after exercise for a total of 40 g/d. Participants exercised 4 times/wk using 3 sets of 6-8 repetitions at 85-90% of the one repetition maximum. Data were analyzed with two-way ANOVA (p < 0.05). The protein supplement resulted in greater increases in total body mass, fat-free mass, thigh mass, muscle strength, serum IGF-1, IGF-1 mRNA, MHC I and IIa expression, and myofibrillar protein. Ten-wks of resistance training with 20 g protein and amino acids ingested 1 h before and after exercise is more effective than carbohydrate placebo in up-regulating markers of muscle protein synthesis and anabolism along with subsequent improvements in muscle performance.

Adolescent↗
PubMed2006

Effects of 28 days of beta-alanine and creatine monohydrate supplementation on aerobic power, ventilatory and lactate thresholds, and time to exhaustion.

The effect of beta-alanine (beta-Ala) alone or in combination with creatine monohydrate (Cr) on aerobic exercise performance is unknown. The purpose of this study was to examine the effects of 4 weeks of beta-Ala and Cr supplementation on indices of endurance performance. Fifty-five men (24.5 +/- 5.3 yrs) participated in a double-blind, placebo-controlled study and randomly assigned to one of 4 groups; placebo (PL, n = 13), creatine (Cr, n = 12), beta-alanine (beta-Ala, n = 14), or beta-alanine plus creatine (CrBA, n = 16). Prior to and following supplementation, participants performed a graded exercise test on a cycle ergometer to determine VO(2peak), time to exhaustion (TTE), and power output, VO(2), and percent VO(2peak) associated with VT and LT. No significant group effects were found. However, within groups, a significant time effect was observed for CrBa on 5 of the 8 parameters measured. These data suggest that CrBA may potentially enhance endurance performance.

Adult↗
PubMed2001

Evaluation of creatine transport using Caco-2 monolayers as an in vitro model for intestinal absorption.

Creatine is a nutraceutical that has gained popularity in both well-trained and casual athletes for its performance-enhancing or ergogenic properties. The major disadvantages of creatine monohydrate formulations are poor solubility and oral bioavailability. In the present study, creatine transport was examined using Caco-2 monolayers as an in vitro model for intestinal absorption. Confluent monolayers of Caco-2 cells (passage 25-35) were used for the permeability studies. Monolayers were placed in side-by-side diffusion chambers. (14)C-Creatine (0.1-0.5 microCi/mL) was added to either the apical or basolateral side, and the transport of the creatine across the Caco-2 monolayer was measured over a 90-min period. The apical to basolateral transport of (14)C-creatine was small, ranging from 0.2-3% of the original amount appearing on the receiver side in a 90-min period. Interestingly, the basolateral to apical permeability of radiolabeled creatine was substantially greater than that observed in the apical to basolateral direction. Studies with drug efflux transport inhibitors indicate that neither the P-glycoprotein nor multidrug resistance-associated protein is involved in the enhanced basolateral to apical transport of creatine.

Biological Transport, Active↗
PubMed2001

Effects of resistance exercise and creatine supplementation on myasthenia gravis: a case study.

PURPOSE: The purpose of this case study was to determine the effects of 15 wk of resistance exercise and creatine (Cr) supplementation on body composition, training volume, peak strength, and complete blood chemistry in a patient with myasthenia gravis (MG). METHODS: The patient was a 26-yr-old man who was taking prednisone and azathioprine for his condition. The patient self-administered 5 g of Cr per day in addition to resistance exercise 3 times per week. Fasting blood samples were obtained and body weight (BW) and fat free mass (FFM; via hydrostatic weighing) were measured before and after training and Cr supplementation. In addition, isokinetic (Cybex II) peak strength for leg extension (LE), leg flexion (LF), and volume load (repetition x mass lifted) for the first and last resistance training session were determined. RESULTS: After Cr supplementation and training, the results demonstrated increases in BW (6.8%), FFM (4.3%), upper body volume load (37.0%), lower body volume load (15.0%), and peak strength for LE (37.0%) and LF (12.5%). Moreover, blood chemistry values remained within normal limits for the duration of the 15-wk study. CONCLUSION: These data suggest that resistance exercise plus Cr supplementation may promote gains in strength and FFM in patients with MG.

Adult↗
PubMed2000

Ligand endocytosis drives receptor dissociation and activation in the Notch pathway.

Endocytosis of the ligand delta; is required for activation of the receptor Notch during Drosophila development. The Notch extracellular domain (NotchECD) dissociates from the Notch intracellular domain (NotchICD) and is trans-endocytosed into delta;-expressing cells in wild-type imaginal discs. Reduction of dynamin-mediated endocytosis in developing eye and wing imaginal discs reduces Notch dissociation and Notch signalling. Furthermore, dynamin-mediated delta endocytosis is required for Notch trans-endocytosis in Drosophila cultured cell lines. Endocytosis-defective delta proteins fail to mediate trans-endocytosis of Notch in cultured cells, and exhibit aberrant subcellular trafficking and reduced signalling capacity in Drosophila. We suggest that endocytosis into delta-expressing cells of NotchECD bound to delta plays a critical role during activation of the Notch receptor and is required to achieve processing and dissociation of the Notch protein.

Animals↗
PubMed1997

Mechanomyographic responses to concentric isokinetic muscle contractions.

The purpose of this investigation was to examine the effects of velocity of contraction on the mechanomyographic (MMG) responses to maximal concentric isokinetic leg extension movements. Eight adult males [mean (SD) age, 22.3 (1.3) years] performed maximal leg extensions on a calibrated Cybex 6000 dynamometer at velocities of 60, 120, 180, 240, 300, and 360 degrees x s(-1) x MMG responses were detected by a piezoelectric recording device placed over the vastus lateralis muscle. Intraclass reliability correlations ranged from R = 0.84 to 0.97 and from 0.90 to 0.99 for peak torque and MMG amplitude values, respectively, with no significant differences (P > 0.05) between the mean values for test versus retest at any contraction velocity. There were significant differences (P < 0.05) in peak torque at all velocities except 240 [135 (27) Nm] versus 300 [127 (27) Nm], and 300 versus 360 degrees x s(-1) [115 (37) Nm]. The mean MMG amplitude at 60 degrees x s(-1) [61 (67) mV] was significantly less (P < 0.05) than that at 360 degrees x s(-1) [452 (451) mV]. These results indicate a velocity-related dissociation between MMG amplitude and peak torque. Furthermore, it was hypothesized that the increases in MMG amplitude were due to velocity-related decreases in muscle stiffness which allowed for greater muscle fiber oscillations.

Adult↗
PubMed1997

Mechanomyography and oxygen consumption during incremental cycle ergometry.

The purpose of this investigation was to describe and compare the relationships for mechanomyography (MMG) and oxygen consumption rate (VO2) versus power output during incremental cycle ergometry. Twenty four adult males [mean (SD) age, 22.1 (2.0) years] volunteered to perform an incremental test to exhaustion on a cycle ergometer. A MMG piezoelectric recording device was placed mid-thigh over the vastus lateralis muscle and VO2 was measured using standard open circuit procedures. The r2 values for the MMG and VO2 versus power output relationships ranged from 0.79 to 0.99 and 0.97 to 0.99, respectively. In 20 of the 24 subjects there was no significant (P > 0.10) difference between the slope values for the normalized MMG and VO2 (expressed as a percentage of maximal values) versus power output relationships. The results of this study indicate that MMG procedures can be used to quantify muscular activity and monitor changes in exercise intensity during cycle ergometry. Furthermore, the present findings demonstrated a close association between the mechanical (MMG) and metabolic (VO2) aspects of muscular contraction during incremental cycle ergometry.

Adult↗
PubMed1997

Validity of near-infrared interactance for estimating relative body fat in female high school gymnasts.

The present investigation examined the validity of near-infrared interactance (NIR) estimates of relative body fat (% fat) from the Futrex-5000 (F5000), Futrex-5000A (F5000 A), and Futrex-1000 (F1000) instruments. Ninety-eight female high school gymnasts (X age+/-SD = 15.7+/-1.2 yr) participated in this investigation. Subsamples were used to cross-validate the F5000 (n = 52), F5000A (n = 46), and F1000 (n = 89) instruments. The NIR % fat estimates were validated against criterion % fat from underwater weighing (UWW) using the adult conversion constants of Brozek et al. (6) (UWWB) and the female age-specific constants of Lohman (23) (UWWL). The cross-validation statistical analysis included examination of the constant error (CE), standard error of estimate (SEE), r, and total error (TE). In addition, full-model multiple regression analyses were used to predict UWWB or UWWL from body weight (BW) and height (HT). BW and HT were correlated with % fat at R = 0.65-0.70, while the validity coefficients for the NIR instruments ranged from r = 0.40-0.78. The F5000 resulted in nonsignificant CE values (-0.3 % fat vs UWWB and 1.5 % fat vs UWWL; p > 0.008) as well as the lowest TE values (TE = 3.1 % fat vs UWWB and 4.0 % fat vs UWWL). All other NIR estimates of % fat resulted in TE values > or = 6.3 % fat. In addition, for all NIR instruments there were negative correlations for the plots of the CE versus the mean of predicted and criterion (UWWB and UWWL) % fat. Therefore, the present findings indicated that the F5000 provided more accurate estimates of % fat than the F5000A or F1000 instruments, but may underestimate the desired minimal body weight for female gymnasts at the low end of the % fat distribution.

Adipose Tissue↗
PubMed1997

Validity of bioelectrical impedence equations for estimating fat-free weight in high school female gymnasts.

The present study examined the validity of bioelectrical impedence (BIA) equations for estimating fat-free weight (FFW) in female gymnasts by comparing the values to those obtained from underwater weighing (UWW). Ninety-seven female Caucasian high school gymnasts (mean age +/- SD = 15.7 +/- 1.1 yr) participated in the study. FFW from UWW was calculated from percent fat using the revised formula of Brozek et al. (mean FFW +/- SD = 43.8 +/- 4.5 kg) and the age-specific constants of Lohman (mean FFW +/- SD = 44.8 +/- 4.6 kg). Cross-validation analyses included examination of the constant error (CE), SEE, r, and total error (TE). The results indicated similar trends between equations when based on either the Brozek or Lohman conversions; however, the CE, SEE, and TE values were consistently lower for the majority of the equations using the revised formula of Brozek et al. Based upon the results of the cross-validation analyses, the equation of Houtkooper et al. and the interlaboratory equations of Van Loan et al. and Lohman, which resulted in identical TE values of 2.4 kg are recommended for use with young high school gymnasts.

Adolescent↗
PubMed1997

Mechanomyographic responses to maximal eccentric isokinetic muscle actions.

The purpose of the present investigation was to examine the mechanomyographic (MMG) responses to maximal eccentric isokinetic muscle actions. Eight adult male volunteers [age 22 +/- 2 (SD) yr] performed maximal eccentric muscle actions of the leg extensors at 60, 90, 120, and 180 degrees /s on a Cybex 6000 isokinetic dynamometer. MMG was detected by a piezoelectric crystal contact sensor placed over the vastus lateralis muscle. Test-retest intraclass correlations ranged from R = 0.88 to 0.97 for peak torque and from R = 0.97 to 0.98 for root mean square MMG amplitude values. There was no significant (P > 0.05) velocity-related change in eccentric peak torque; however, there was a significant (P < 0.05) increase in MMG between 60 [119 +/- 44 (SE) mV] and 180 degrees/s (302 +/- 128 mV). These findings indicated a velocity-related dissociation between MMG and peak torque for maximal eccentric isokinetic muscle actions.

Adult↗
PubMed1996

Effect of workbout duration on the physical working capacity at fatigue threshold (PWCFT) test.

The purpose of this study was twofold: (1) to determine the effect of increasing the duration of the workbout at each power output during the physical working capacity at fatigue threshold (PWCFT) test from 2 to 3 or 4 min, and (2) to examine the time to exhaustion during continuous workbouts at the PWCFT. Twelve adult males (means +/- SD = 22.4 +/- 3.0 years) volunteered to perform three PWCFT tests using workbout durations of 2, 3, and 4 min. Following the determination of the PWCFT values, nine of the subjects performed continuous workbouts at PWCFT2 and PWCFT4 for as long as possible. The mean PWCFT value using 4-min workbouts (PWCFT4 = 168.8 +/- 45.1 W) was significantly less (p < 0.05, 19.1%) than that using 2-min workbouts (PWCFT2 = 208.9 +/- 59.0 W). However, only two subjects were able to complete 60 min at PWCFT4 and none of the subjects were able to complete 60 min at PWCFT2. Therefore, although increasing the duration of the workbout at each power output resulted in a lower PWCFT4, these findings do not support a recommendation for a change in the PWCFT test protocol.

Adult↗
PubMed1996

Validity of bioelectrical impedance equations for estimating percent fat in males.

The present study examined the validity of selected bioelectrical impedance (BIA) equations for estimating percent fat (% fat) in males and compared their validity with that of a commonly used skinfold equation (Sum3). One-hundred twenty-two Caucasian males (X +/- SD = 12.5 +/- 5.8% fat, as determined by underwater weighing) served as subjects. Selection of the BIA equations was dependent upon meeting at least one of three criteria: 1) developed from a previous interlaboratory investigation, 2) derived on a large sample size (> 200), or 3) previously been shown to accurately estimate body composition when cross-validated against a criterion method. Cross-validation analyses included examination of the constant error, standard error of estimate (SEE), r, and total error (TE). The Sum3 equation which resulted in the lowest SEE and TE values (2.6% fat and 3.4% fat, respectively) and the highest validity coefficient (r = 0.90, P < 0.001), most accurately estimated % fat and, therefore, was recommended over BIA equations for estimating body composition in Caucasian males with lean to average body fatness. The fat-specific interlaboratory BIA equation of Segal et al. for males < or = 20% fat (N = 107) which resulted in a TE value of 3.6% fat and the generalized equation of Guo et al. (TE = 4.1% fat) may, however, be considered as acceptable alternatives.

Adipose Tissue↗
PubMed1996

Age, fat-free weight, and isokinetic peak torque in high school female gymnasts.

The purpose of this study was to examine the covariate influence of fat-free weight (FFW) on age-related increases in isokinetic peak torque for leg flexion and extension in high school female gymnasts. Seventy-two gymnasts (X age +/- SD = 15 7 +/- 1.2 yr) volunteered to be measured for isokinetic leg flexion and extension strength using a calibrated Cybex II dynamometer at 30, 180, and 300 degrees*s(-1) as well as for body composition from underwater weighing. The results indicated that there were significant (P < 0.05) zero-order correlations for age versus leg flexion (r = 0.36-0.47) and extension (r = 0.51-0.57) peak torque, as well as FFW versus leg flexion (r = 0.50-0.66) and extension (r = 0.620.73) peak torque. There were also significant (P < 0.05) first-order partial correlations between age and peak torque (covaried for FFW) for leg extension at 30 (r = 0.25), 180 (r = 0.36-0.39), and 300 degrees*s(-1) (r = 0.25-0.28) but not for leg flexion. These findings indicated that for the high school female gymnasts in the present study, there were age-related increases in strength that could not be accounted for by changes in FFW. It is possible that factors such as an increase in muscle mass per unit of FFW and/or neural maturation contribute to strength increases during adolescence in female athletes.

Adolescent↗
PubMed1996

Validity of skinfold estimates of percent fat in high school female gymnasts.

A preseason estimation of body composition may be useful for assigning a safe minimal body weight for female gymnasts. The present investigation examined the validity of 11 skinfold equations for predicting percent body fat (%fat) in high school female gymnasts (X age +/- SD = 15.7 +/- 1.2 yr) by comparing the values with those obtained from underwater weighing. Seventy-three gymnasts (X %fat +/- SD = 18.6 +/- 4.5%fat) volunteered to serve as subjects. The statistical analyses included examination of the constant error (CE), standard error of estimate (SEE), correlation coefficient (r), and total error (TE). The results of this investigation indicated that 7 of the 11 equations resulted in TE values that were < or = 3.9%fat (range, 3.3-3.9%fat). Of these, the quadratic sum-of-three skinfold equation of Thorland et al. (37) satisfies the most cross-validation criteria and, therefore, is recommended for estimating body composition and minimal body weight in high school female gymnasts. The other six equations with TE values of < or = 3.9%fat should be considered acceptable alternatives.

Adipose Tissue↗
PubMed1996

Neuromuscular fatigue thresholds of the vastus lateralis, vastus medialis and rectus femoris muscles.

The purpose of this study was to compare the power outputs associated with the onset of neuromuscular fatigue in the superficial muscles of the quadriceps femoris group by simultaneously monitoring the EMG activity from the vastus lateralis (VL), vastus medialis (VM) and rectus femoris (RF) muscles during a Physical Working Capacity at the Fatigue Threshold (PWCFT) test. Eleven adult males (mean +/- SD = 24 +/- 3y) served as subjects. There were no significant (p > 0.05) differences among the mean PWCFT values for the VL (226 +/- 58 W), VM (223 +/- 58 W) and RF (203 +/- 54 W) muscles. These findings indicates that the superficial muscles of the quadriceps femoris group generally responded as a unit with respect to the onset (threshold) of neuromuscular fatigue as measured by the PWCFT test.

Adult↗
PubMed1995

Electromyographic fatigue thresholds of the superficial muscles of the quadriceps femoris.

The purpose of this investigation was to compare the thresholds of neuromuscular fatigue determined simultaneously from the vastus lateralis (VL), vastus medialis (VM) and rectus femoris (RF) muscles using the electromyographic fatigue threshold (EMGFT) test. Eight adult volunteers [mean (SD) age, 33 (10) years] served as subjects for this investigation. The results of a one-way repeated measured ANOVA indicated that there was a significant (P < 0.05) difference among the mean EMGFT values for the VL [248(31)W], VM [223(43)W] and RF [220(30)W] muscles. Tukey post-hoc comparisons indicated that the EMGFT for the RF was significantly (P < 0.05) lower than that of the VL. These findings suggested that during cycle ergometry there is a dissociation in neuromuscular fatigue characteristics of the superficial muscles of the quadriceps femoris group.

Adult↗