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Biomedical subjects

J R Wu

Publications and source records attributed to J R Wu.

At least 73 records · Page 4Linked to original sources

A trial of adenosine for the termination of supraventricular tachycardia in infancy: a case report.

A 40-day-old male infant was admitted due to cough, anorexia and pale face. Rapid heart beat was found accidentally by auscultation. Electrocardiograms revealed supraventricular tachycardia (SVT) with a heart rate up to 250 beats per minute, and an absence of P waves. Intravenous bolus injection of 1 mg adenosine terminated the tachycardia, followed by transient complete atrioventricular block, then latent Wolff-Parkinson-White (WPW) syndrome with retrograde P waves. Adenosine may be a new trial for the termination of SVT, and also is a safe drug even in the presence of WPW syndrome.

Adenosine↗

Radiation exposure of pediatric patients and physicians during cardiac catheterization and balloon pulmonary valvuloplasty.

Thermoluminescent dosimeters were applied to various areas of 61 pediatric patients and physicians to measure radiation doses during routine cardiac catheterization and during 4 cases of balloon pulmonary valvuloplasty. Radiation doses were measured during chest roentgenography, fluoroscopy and cineangiography. Average skin dose to the chest was 121 microGy during chest x-ray, 5,182 microGy during catheterization and 641 mGy during valvuloplasty. For the eyes, thyroid and gonads of the patients, the exposure during routine catheterization was equal to 0.4, 6 and 0.2 chest x-rays, respectively. Radiation dose of the operator was 3 microGy for the eyes and 6 miCroGy in the thyroid. About 56% of the operator's dose could be reduced by thyroid shields, and 80% by lead aprons. The assistant received only 1 microGy outside the thyroid shield. Therefore, we have concluded that the patients' dose during routine catheterization is largely based on our experimental results, but the dose is acceptable based on the risk factor analysis. The skin dose to the right lateral chest of the patient during valvuloplasty is extremely high, perhaps as high as the equivalent of 1,000 chest x-rays. Besides the clinical benefits of valvuloplasty, the long-term radiation-related hazards to the patient should be carefully monitored.

Adolescent↗

Mechanism of poly(ethylene glycol)-induced lipid transfer between phosphatidylcholine large unilamellar vesicles: a fluorescent probe study.

Experiments were performed to assess three possible mechanisms of poly(ethylene glycol) (PEG) induced rapid lipid transfer between large unilamellar vesicles composed of dioleoylphosphatidylcholine: (1) transfer between aggregated vesicles, (2) transfer through an aqueous medium of lowered dielectric constant, and (3) transfer via a PEG carrier. The results showed that close contact between vesicles as a result of PEG dehydration was largely responsible for the rapid lipid transfer observed in the presence of PEG. The rate and extent of lipid transfer were also examined at 10 wt % PEG and analyzed in terms of a two-state model especially developed to account for the initial rate of lipid transfer as followed by the fluorescence lifetime of DPHpPC as a fluorescent lipid probe. Analysis revealed that two rate processes were involved in DPHpPC transfer between bilayers, both in the absence and presence of PEG. Since the maximum extent of transfer was 50%, transbilayer diffusion of DPHpPC seemed not to contribute to either process. The fast process in the presence of PEG was identified as due to rapid interbilayer monomer diffusion between closely apposed vesicles, and, in the absence of PEG, as due to monomer diffusion through the aqueous phase. The origin of the slow process, in both cases, remains obscure. The Arrhenius activation energies (and entropies) for the initial rates at temperatures from 10 to 48 degrees C were 15.3 +/- 0.3 kcal/mol (-26.3 +/- 0.2 eu) and 10.6 +/- 0.5 kcal/mol (-16.1 +/- 0.3 eu) in the absence and presence of PEG, respectively. The slow process was invariant with temperature.(ABSTRACT TRUNCATED AT 250 WORDS)

Dialysis↗

Reversed-phase high-performance liquid chromatography of amphoteric beta-lactam antibiotics: effects of columns, ion-pairing reagents and mobile phase pH on their retention times.

The separation of five amino beta-lactam antibiotics by reversed-phase high-performance liquid chromatography was studied as an insight into their retention behaviour. These five amphoteric compounds are cephradine, cephalexine, cefaclor, ampicillin and amoxicillin. Both octadecylsilane-bonded silica (C18) columns and phenyl-bonded silica (phenyl) columns were used, with mobile phase pH values between 2.5 and 7.4. In the absence of ion-pairing reagents the retention times for all the five compounds were the shortest at pH 4-6. The phenyl column was found to improve the separation between cephradine and ampicillin at pH values lower than 3, when these two compounds appeared as fused peaks on the C18 on C18 columns, with mobile phases both with and without ion-pairing reagents, were compared. The addition of 0.005 or 0.02 M tetraethylammonium acetate to the mobile phase did not result in significant ion-pair formation, except at pH values higher than 5.5. A strong ion-pairing effect was obtained at pH values higher than 6 with 0.005 or 0.02 M tetrabutylammonium phosphate, and the retention was decreased at pH values lower than 4. On the other hand, 0.005 M heptanesulphonic acid exhibited an ion-pair retention effect at pH values lower than 5. The molecular structures and pK(a) values were used to account for the retention behaviour of these antibiotics in the various mobile phases.

Amoxicillin↗

Fourier transform infrared spectroscopic study of Ca2+ and membrane-induced secondary structural changes in bovine prothrombin and prothrombin fragment 1.

Fourier transform infrared (FTIR) spectroscopy was used to monitor secondary structural changes associated with binding of bovine prothrombin and prothrombin fragment 1 to acidic lipid membranes. Prothrombin and prothrombin fragment 1 were examined under four different conditions: in the presence of (a) Na2EDTA, (b) 5 mM CaCl2, and in the presence of CaCl2 plus membranes containing 1-palmitoyl-2-oleoyl-3-sn-phosphatidylcholine (POPC) in combination with either (c) bovine brain phosphatidyl-serine (bovPS) or (d) 1,2-dioleoyl-phosphatidylglycerol (DOPG). The widely reported Ca(2+)-induced conformational change in bovine prothrombin fragment 1 was properly detected by our procedures, although Ca(2+)-induced changes in whole prothrombin spectra were too small to be reliably interpreted. Binding of prothrombin in the presence of Ca2+ to procoagulant POPC/bovPS small unilamellar vesicles produced an increase in ordered secondary structures (2% and 3% increases in alpha-helix and beta-sheet, respectively) and a decrease of random structure (5%) as revealed by spectral analysis on both the original and Fourier-self-deconvolved data and by difference spectroscopy with the undeconvolved spectra. Binding to POPC/DOPG membranes, which are less active as procoagulant membranes, produced no detectable changes in secondary structure. In addition, no change in prothrombin fragment 1 secondary structure was detectable upon binding to either POPC/bovPS or POPC/DOPG membranes. This indicates that a membrane-induced conformational change occurs in prothrombin in the nonmembrane-binding portion of the molecule, part of which is activated to form thrombin, rather than in the membrane-binding fragment 1 region. The possible significance of this conformational change is discussed in terms of differences between the procoagulant activities of different acidic lipid membranes.

Animals↗

Membrane binding induces lipid-specific changes in the denaturation profile of bovine prothrombin. A scanning calorimetry study.

Prothrombin denaturation was examined in the presence of Na2EDTA, 5mM CaCl2, and CaCl2 plus membranes containing 1-palmitoyl-2-oleoyl-3-sn-phosphatidylcholine (POPC) in combination with either bovine brain phosphatidylserine (PS) or 1,2-dioleoyl-phosphatidylglycerol (DOPG). Heating denaturation of prothrombin produced thermograms showing two peaks, a minor one at approximately 59 degrees C previously reported to correspond to denaturation of the fragment 1 region (Ploplis, V. A., D. K. Strickland, and F. J. Castellino 1981. Biochemistry. 20:15-21), and a main one at approximately 57-58 degrees C, reportedly due to denaturation of the rest of the molecule (prethrombin 1). The main peak was insensitive to the presence of 5mM Ca2+ whereas the minor peak was shifted to higher temperature (Tm approximately 65 degrees C) by Ca2+. Sufficient concentrations of POPC/bovPS (75/25) large unilamellar vesicles to guarantee binding of 95% of prothrombin resulted in an enthalpy loss in the main endotherm and a comparable enthalpy gain in the minor endotherm accompanying an upward shift in peak temperature (Tm approximately 73 degrees C). Peak deconvolution analysis on the prothrombin denaturation profile and comparison with isolated prothrombin fragment 1 denaturation endotherms suggested that the change caused by POPC/PS vesicles reflected a shift of a portion of the enthalpy of the prethrombin 1 domain to higher temperature (Tm approximately 77 degrees C). The enthalpy associated with this high-temperature endotherm increased in proportion to the surface concentration of PS. By contrast, POPC/DOPG (50/50) membranes shifted the prethrombin 1 peak by 4 degrees C to a lower temperature and the fragment 1 peak by 5 degrees C to a higher temperature. The data lead to a hypothesis that the fragment 1 and prethrombin 1 domains of prothrombin do not denature quite independently and that binding of prothrombin to acidic-lipid membranes disrupts the interaction between these domains. It is further hypothesized that PS containing membranes exert the additional specific effect of decoupling the denaturation of two subdomains of the prethrombin 1 domain of prothrombin.

Animals↗

Measurements of the effective nonlinearity parameter B/A of water containing trapped cylindrical bubbles.

The effective nonlinearity parameter B/A of water containing a three-dimensional ensemble of randomly distributed uniform-sized trapped cylindrical bubbles was measured. The experimental results indicate that the dramatic enhancement of the effective nonlinear parameter B/A is due to the nonlinear resonance oscillation of the trapped bubbles. The measured effective B/A for the system is of the magnitude of 10(4) to 10(5).

Mathematics↗

Acoustical tweezers.

A stable force potential well was generated by two collimated focused ultrasonic (3.5 MHz) beams propagating along opposite directions. Latex particles (270-microns diameter) and clusters of frog eggs were trapped in the potential well. The trapped object can be moved axially or laterally by moving one of the PZT focusing transducers that generate the ultrasonic focused beams. The axial position of the trapped object can also be maneuvered by tuning the frequency of the electrical voltage applied on the transducers.

Animals↗

[An investigation of radiation exposure on pediatric patients and doctors during cardiac catheterization and cineangiography].

Cardiac catheterization and cineangiography are invasive diagnostic radiological examinations which exposure the patient to a relatively high radiation dose, the largest radiation dose of any diagnostic examination. Sixty-one infants and children, aged 28 days to 15 years, were randomly selected into this study to assess such questions regarding cardiac catheterization in children as; (1) How much the pediatric patient's and doctor's radiation exposure is during a routine cardiac catheterization and cineangiography? (2) What difference are there in exposure to various areas of the body? (3) How much radiation exposure is related to cineangiography compared with fluoroscopy? (4) How much radiation exposure may be reduced by lead apron worn by doctors? Thermoluminescent dosimeters containing 80 mg of CaSO4: Dy were applied to the body for the measurement of radiation exposure during cardiac catheterization. In hemodynamic assessment of catheterization (mean time 6 +/- 5 min), dosimeters were placed on various areas of the patient's skin and unexposed dosimeters were attached to mid sternum and right lateral chest during cineangiography (mean time 11 +/- 5 sec). Average skin doses over the patients' organs during catheterization and cineangiography were: eye 51 mu Gy, thyroid 746 mu Gy, sternum 5,102 mu Gy, right lateral chest 10,098 mu Gy, umbilicus 123 mu Gy and gonad 24 mu Gy. The radiation exposure during cineangiography at sternum and right lateral chest contributed to 61% and 70% of total exposure.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗

[Permanent pacemaker with implantation in a young child with congenital complete atrioventricular block: a case report].

Congenital atrioventricular block is a congenital disorder of the cardiac conducting system. Pacemaker implantation is the treatment of choice for patients with congenital A-V block if prior medical treatment fails. The criteria for pacemaker implantation in infants and children are (1) frequent syncope (2) heart failure (3) ventricular rate below 55 beats/min (4) ventricular arrhythmia (5) QRS widening in the ECG (6) moderate to severe exercise intolerance. Our experience with a 15-month-old male child is herein presented. The patient suffered from frequent attacks of upper respiratory tract infection, bradycardia and complete A-V block as found on electrocardiograms. At 15 months old, he was admitted again due to respiratory tract infection and several attacks of syncope. Stokes-Adams syndrome was noted several times despite medical treatment. Following implantation of a VVI mode epicardiac pacemaker, the patient's condition, including resistance to heart failure and respiration, were improved dramatically. He was followed up regularly for 4 years, his heart beat remaining stable.

Follow-Up Studies↗

Temperature elevation generated by a focused Gaussian beam of ultrasound.

The steady state temperature elevation generated by a focused Gaussian beam, including the effect of perfusion, has been calculated along the beam axis. The medium is assumed to be a homogeneous absorbing one. The results indicate: (1) The temperature rise is an increasing function of the intensity gain of the focusing transducer, but never seems to exceed twice that at the interface of the transducer and the medium generated by its unfocused counterpart; and (2) The temperature rise at the interface of the transducer and the medium is not affected significantly by focusing.

Hot Temperature↗

Acoustic radiation pressure on a rigid cylinder: an analytical theory and experiments.

Simple and general analytical expressions are derived for the acoustic radiation force on a long rigid cylinder with a small diameter, whose axis is perpendicular to the wave propagation. Results are expressed in terms of the time-averaged densities of kinetic and potential energies of the incident sound field. For the case of a standing-wave field, which was used in these experiments, the theoretical results agree well with the experimental observations.

Acoustics↗

The distribution of interspersed repetitive DNA sequences in the human genome.

The distribution of interspersed repetitive DNA sequences in the human genome has been investigated, using a combination of biochemical, cytological, computational, and recombinant DNA approaches. "Low-resolution" biochemical experiments indicate that the general distribution of repetitive sequences in human DNA can be adequately described by models that assume a random spacing, with an average distance of 3 kb. A detailed "high-resolution" map of the repetitive sequence organization along 400 kb of cloned human DNA, including 150 kb of DNA fragments isolated for this study, is consistent with this general distribution pattern. However, a higher frequency of spacing distances greater than 9.5 kb was observed in this genomic DNA sample. While the overall repetitive sequence distribution is best described by models that assume a random distribution, an analysis of the distribution of Alu repetitive sequences appearing in the GenBank sequence database indicates that there are local domains with varying Alu placement densities. In situ hybridization to human metaphase chromosomes indicates that local density domains for Alu placement can be observed cytologically. Centric heterochromatin regions, in particular, are at least 50-fold underrepresented in Alu sequences. The observed distribution for repetitive sequences in human DNA is the expected result for sequences that transpose throughout the genome, with local regions of "preference" or "exclusion" for integration.

Chromosome Mapping↗

Experimental frequency spectra of ultrasound transmission through two-dimensional ensembles of trapped microbubbles.

The frequency spectra of transmission coefficients for ultrasound passing through a sheet of gas-filled micropores have been measured using incident waves with amplitudes up to 2.4 x 10(4) Pa. It is found that as the amplitude of the incident wave is increased, the peak frequency of transmission loss through the two-dimensional trapped-bubble ensemble shifts to lower values by as much as 47%. The experiments indicate that the shifting is caused by the net displacements of air-water-membrane triple-phase lines and the air loss of the trapped bubble, which are due to the radiation force and microbubble production produced by the incident wave. Results of the experiments and possible theoretical explanations are discussed.

Ultrasonics↗

[Pulmonary artery sling: diagnosis by magnetic resonance imaging--one case report].

Pulmonary artery sling is an uncommon congenital vascular anomaly, which may cause significant respiratory morbidity resulting from an external tracheal compression during early infancy. This condition should be considered on finding a mass interposed between the trachea and the esophagus showing an anterior indentation on the esophagograms. The diagnosis is usually made by invasive methods such as bronchography, bronchoscopy, cardiac catheterization, and angiography. Magnetic resonance imaging (MRI) has now become an excellent method for diagnosing cardiovascular anomalies, but the use of MRI as a means of diagnosing pulmonary artery sling is rare. In 1988, Malmgren et al, first reported the use of MRI in three cases. Their findings suggested that two overlaping sets of axial sections might be necessary for depicting the anomaly because different parts of the left pulmonary artery are imaged in different slices. In this paper, we report a diagnostic scan image which utilizes a single axial scan and reveals the pulmonary artery sling arising aberrantly from the right pulmonary artery. We also found (a) when the pulmonary artery sling interposed between the esophagus and trachea; the trachea become stenotic below the upper one-third in LAO equivalent sagittal section; (b) the right pulmonary artery compresses the proximal part of right pulmonary bronchus, and the right upper lobe bronchus arises from the trachea independently in the coronal section. Thus, we conclude that MRI, if available, should be the first choice modality for further examination when a pulmonary artery sling is suggested by conventional radiography.

Female↗

[Right ventricular thrombus mimicking a tumor mass in a newborn with pulmonary atresia--an autopsy report].

Intracardiac masses in infancy are not common. Tumors, thrombi and vegetations of bacterial endocarditis are exceedingly rare in this age group. These masses are seldom diagnosed before the infant's death. Two-dimensional echocardiography is a noninvasive technique that can detect and differentiate intracardiac masses that occur in infants. It can serially detect changes in the size or location of the masses, thus guiding subsequent medical or surgical intervention. An 11-day-old male newborn had suffered from lip and finger nail cyanosis, and dyspnea since 2 days old. Physical examination revealed subcostal retractions and no heart murmur during admission but a grade II/VI systolic murmur with a sometime grade II/VI continuous murmur which could be heard at the left upper sternal border 3 days after admission. The liver was palpable 2 cm below the right costal margin. The electrocardiogram was normal. The chest X-ray showed a normal sized heart (CTR = 0.58) with decreased pulmonary vascularity. Arterial blood gases revealed hypoxemia (PO2 24.1 mmHg) and metabolic acidosis, even though the patient was receiving O2 at 50%. The two-dimensional echocardiogram from the parasternal long axis view showed an echo-dense tumor mass in the right ventricular cavity. At autopsy, a complex congenital heart disease was found. The pathologic findings were: patent ductus arteriosus (1.0 x 0.5 cm), fenestrated foramen ovale, unopening pulmonary valve with thrombus (0.3 x 0.2 cm), right ventricular hypoplasia and hypertrophy of the right ventricular wall (1 cm in thickness). A ball like, organized and calcified thrombus, measuring 2.0 x 1.3 x 1.0 cm was found in the right ventricular cavity.(ABSTRACT TRUNCATED AT 250 WORDS)

Abnormalities, Multiple↗