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J Ralph

Publications and source records attributed to J Ralph.

10 recordsLinked to original sources
PubMed1995

Obsessive-compulsive symptoms and the family.

A questionnaire study of 98 family members of individuals with obsessive-compulsive symptoms revealed that 60% of the family members were involved to some extent in rituals with the affected family member. Nearly all family members reported at least some degree of interference in their lives. Information was also gathered about the sort of rituals in which members were involved, how they responded to the demands of the affected relative to engage in the rituals, their beliefs and knowledge about compliance, and the degree to which the rituals interfered in their lives. The study highlights the possible need for support, advice, and education for family members of persons with obsessive-compulsive symptoms.

Adolescent↗
PubMed1993

Synthesis and spectroscopic characterization of hydroxycinnamoylated methyl alpha-L-arabinofuranosyl-(1-->2)- and (1-->3)-beta-D-xylopyranosides.

A reaction sequence for the preparation of methyl 5-O-feruloyl-alpha-L- arabinofuranosyl-(1-->3)-beta-D-xylopyranoside, the companion 5-O-p-coumaroyl disaccharide, and their (1-->2) analogs has been developed. The (1-->3) hydroxycinnamoylated disaccharides are available in 11 steps from L-arabinose and methyl beta-D-xylopyranoside in 17% overall yield (based on methyl beta-D-xylopyranoside). The corresponding (1-->2) materials were prepared in 9 steps in > 37% overall yield. Complete spectral characterization provides unambiguous assignments for comparison with analogous materials isolated from plant cell-walls. Conformational aspects of the prepared materials are discussed in relation to coupling-constant information.

Arabinose↗
PubMed1991

Synthesis of methyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside and its use as a substrate to assess feruloyl esterase activity.

A synthetic scheme was developed for the production of methyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside (FA-Ara) in gram quantities. This molecule accurately models the chemical attachment of ferulic acid to polysaccharides found in cell walls of plants in the Gramineae family. It is therefore a realistic substrate that can be used to monitor feruloyl esterase activity. Ultraviolet spectral analysis indicated that FA-Ara has an absorption maximum distinct from the hydrolytic product, ferulic acid (FA), over a wide range of solution pH values. The log molar extinction coefficient ranges from 4.16 to 4.36 for FA-Ara and 4.16 to 4.33 for FA depending upon the pH of the buffered solution. Consequently a convenient spectrophotometric assay can be utilized to monitor esterase activity. Three different methods were developed for using this model substrate to assess esterase activity, including thin-layer chromatography, a spectrophotometric assay, and the use of high-performance liquid chromatography.

Arabinose↗
PubMed1990

Synthesis of affinity ligands and radioactive probes for isolation and study of myo-inositol 1,4,5-trisphosphate binding proteins.

To synthesize an affinity matrix for isolation of D-myo-inositol 1,4,5-trisphosphate binding proteins, racemic 3-cyclohexene-1-carboxaldehyde was oxidized and converted to a mixture of trans-3,4-di-hydroxycyclohexane-1-carboxylic acid methyl ester isomers, which was phosphorylated and separated into (+-)-(1R,3R,4R)- and (+-)-(1R,3S,4S)-trans-3,4-bis[(diphenoxyphosphoryl)oxy]cyclohex an e-1- carboxylic acid methyl esters. Each of these racemic compounds was hydrogenolyzed and reacted with ethylenediamine to give a monoamide, N-(2-aminoethyl)-bis(phosphonyloxy)cyclohexane-1-carboxamide, that was coupled to cyanogen bromide activated Sepharose 4B to provide the desired affinity matrices. The intermediate trans-3,4-bis[(diphenoxyphosphoryl)oxy]cyclohexane-1-carboxylic acid methyl ester was also reduced with lithium borotritide to give the (hydroxy[3H]methyl)cyclohexane derivative, which was phosphorylated and hydrogenolyzed to yield trans-3,4-bis(phosphonyloxy)-1-[(phosphonyloxy)[3H]methyl]cy clohexane, a radiolabeled analogue of inositol 1,4,5-trisphosphate. The carboxamide was also coupled to 4-azidosalicylic acid, and the product was iodinated to provide a 125I-radiolabeled photoactivatable cross-linking derivative of cyclohexanediol bisphosphate.

Calcium Channels↗
PubMed1989

Morphological alterations in cultured neuromuscular tissue induced by two anesthetic agents.

A diversity of pathogenic effects was observed in two complementary culture systems following their exposure to the anesthetic agents. Thiopental sodium and ketamine hydrochloride. The cytotoxic effects of both agents in these two culture types were reversible and dose-related. In organotypic spinal cord slice cultures, thiopental sodium caused general toxicity but no demyelination, while ketamine hydrochloride induced, to a varied extent, damage of the myelin sheath and degeneration of mitochondria into multilamellar bodies. In autologous nerve-muscle co-cultures both anaesthetic agents caused the arrest of muscle contractions. However, when added to skeletal muscle cultures, the drugs differed in their effect. Thiopental sodium did not inhibit spontaneous muscle contractions indicating, as in the case of Tubocurarine, a direct effect of the drug on the neuromuscular junction. Ketamine hydrochloride, in contrast, arrested spontaneous muscle contractions, implying that it did not directly affect the neuromuscular synapse.

Anesthetics↗
PubMedDate not supplied

Hardiness, stress, and burnout among intensive care nurses.

The purposes of this study were to determine whether hardiness is a predictor of burnout and whether it can buffer the effect of stress on burnout. Thirty-one registered nurses who work in intensive care units completed the Hardiness test, the Nursing Stress scale, and the Tedium scale. Descriptive statistics, correlational statistics, t tests, analysis of variance, and hierarchical multiple regressions were used to analyze the data. Results indicated that hardiness and burnout had a significant inverse relationship (r = 0.66). Stress and the stress-hardiness interaction term accounted for 29% of the variance in burnout scores. This study found hardiness to be a predictor of burnout and a buffer in the stress-burnout relationship. Furthermore, the relationship between hardiness and stress was found to be stronger than that between stress and burnout. Further study is needed to verify that hardiness is a stress mediator in nursing and to determine how to best promote hardiness in nurses.

Adult↗
PubMedDate not supplied

Enhancing the coping skills of mothers with developmentally delayed children.

Recent studies have shown that mothers of developmentally delayed children are at risk for depression. During weekly psychoeducational group meetings, Roy's adaptation model was used to identify the focal, contextual, and residual stimuli that contributed to the mothers' depression. Nursing interventions that encouraged adaptation in the four modes were implemented by group facilitators providing parent education and emotional support. Implications for nurses in a variety of settings are given.

Adaptation, Psychological↗
PubMedDate not supplied

Operation cataract.

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Aged↗