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J Reina Prieto

Publications and source records attributed to J Reina Prieto.

10 recordsLinked to original sources
PubMed1998

[Usefulness of urine and blood cultures for diagnosing cytomegalovirus infection in the kidney transplant recipient].

OBJECTIVE: To prospectively and comparatively study the usefulness of urine (viruria) and blood (antigenemia pp65 and culture) (viremia) for the diagnosis of cytomegalovirus (CMV) infection in renal transplant (RT) recipients. MATERIAL AND METHODS: All RT recipients at our hospital were studied from January 1995 to December 1996. After decontamination, urine specimens were inoculated into two MRC-5 cell line vials. Polymorphonuclear cells were extracted from peripheral blood by sedimentation in saline dextran and were used for antigenemia pp65 test and for culture in shell-vial. RESULTS: A total of 1,335 specimens from 43 patients were studied. CMV was recovered from 110 out of the 913 (12%) urine specimens and from 101 out of the 422 (23.9%) blood specimens (antigenemia and/or viremia). CMV detection was first obtained by a positive blood test in 23 patients (88.4%), whereas the urine specimen was the first positive test in only three (11.6%) patients (p = 0.0001). A positive result in blood preceded a positive result in urine by a mean of 10.3 days (range: 2-73 days). Antigenemia and viremia were simultaneously positive in 61.5% of patients. In three patients a positive antigenemia preceded viremia by 14 days. In seven patients (26.9%) only the shell-vial culture was positive. Culture preceded antigenemia by a mean of 7.6 days. In the 26 patients, the time elapsed until the first positive blood specimen for CMV was 37.3 days (range: 11-88 days). CONCLUSION: According to the results obtained we believe that blood (antigenemia pp65 and/or viremia) should be considered as the only really useful specimen for the diagnosis of infection/disease caused by CMV in RT recipients. The urine specimen lacks a diagnostic and clinical usefulness and therefore should not be used in these patients.

Antigens, Viral↗
PubMed1993

[Isolation of Ureaplasma urealyticum in respiratory samples from two patients with bronchiolitis].

We report the cases of two patients, 5 and 4 months of age, without clinically important antecedents, that were hospitalized with a bronchiolitis syndrome. In the respiratory samples (nasopharynx aspirates) of both patients we isolated Ureaplasma urealyticum in pure culture and with a quantitative count > 10(3) cfu/ml. Vaginal cultures of mothers were negative. The patients were treated with fluid therapy and salbutamol, and after the respiratory cultures were reported positive, with erythromycin for 7 days. Because of the age of the patients (not in the neonatal period) and the type of respiratory syndrome (bronchiolitis), it is very difficult to evaluate the real clinical significance of these isolations. We review the respiratory pathology caused by U. urealyticum during the neonatal period and we recommend the need for a multicenter study about the clinical significance of this microorganism in children under one year of age.

Bronchiolitis↗
PubMed1992

[Neonatal sepsis caused by Streptococcus pneumoniae. Report of two cases].

Streptococcus pneumoniae is an unusual pathogen during the neonatal period. Two cases of neonatal early-onset sepsis, one of them associated with meningitis, are reported. Positive cultures for Strep. pneumoniae were obtained from both newborns and their mothers. Both newborns were full term with birth weights in the normal range. In one of them, amniorrexis occurred 18 hours before the delivery and the amniotic fluid was meconium stained. Significant clinical findings consisted in fever and respiratory distress. There was leucopenia and in one case the chest radiography was abnormal. Both neonates had an uneventful recovery after starting antibiotic treatment and no long term sequellae were detected. The incidence of neonatal sepsis caused by Strep. pneumoniae and its pathogenesis are reviewed.

Ampicillin↗
PubMed1989

[Gastroenteritis caused by enteric adenovirus serotypes 40 and 41. Preliminary study].

We present the preliminary results of a prospective study of childhood gastroenteritis associated with enteric adenovirus (AE-40 and AE-41). In a period of five months we have studied 499 children with enteritis; 9 (1.8%) were positive for enteric adenoviruses (latex agglutination). The mean age of the children was 16.2 months (extreme ages 10 days and 5 years). The mean duration of diarrhoea was 2.8 days with 4-5 times daily. Respiratory symptoms were present in 44.4% of the patients and one patient was infected simultaneously with Campylobacter jejuni. All clinical syndromes were self-limiting and were treated with diet and rehydration.

Adenoviridae Infections↗
PubMed1989

[Childhood shigellosis: clinical and microbiological characteristics].

Authors present a retrospective study on 89 cases of pediatric shigellosis detected between 1983-1987. Most frequent isolated strain was S. sonnei with 67.4% and S. flexneri with 31.4% cases. 73.9% of all isolements were marked between the months october-december. Mean age of patients was 4.4 years and 44.9% of them need not to be hospitalized. Only 3.3% of patients presented a clinical syndrome of bacillar disentery with blood and mucus. Strains were resistent to ampicilin in 84.2% and to thrimetoprim-sulphimethosaxol in 80.9% of cases.

Anti-Bacterial Agents↗
PubMed1980

[Viruses and gastroenteritis (author's transl)].

In recent years it has been demonstrated that a group of viruses, Rotavirus for the children and Norwalk agent for adults, are a highly significant cause of acute gastroenteritis during the months of winter ("winter vomiting disease"). The Rotavirus was identified by Bishop et al. as a double-stranded RNA virus that can be isolate from faeces of children with acute gastroenteritis. Viral gastroenteritis is an autolimitate disease, although under certain conditions it may even lead to severe disease and death by profound dehydration and electrolyte imbalance; it is characterized by nausea, vomiting, diarrhea and others minor symptoms. This entity only affects children between 3 months and 6 years of age, with a little prevalence for the males. Breast-fed babies are commonly thought to be less likely than artificial-fed babies to suffer from infective diarrhea. Rotavirus infection is also known to occur in parents of infected children, but in this situation the symptoms are generally mild or absent and they needn't medical attention. The Rotavirus invade mainly the epithelium of the proximal intestine where they make a cytopatic and physiologic alteration (deficience in Na and K-ATPsa activity) that determines a disturbances of water and electrolyte transport across the epithelium thus contribute to the diarrhea. The best methods for detecting this type of viral gastroenteritis are electron microscopy and, specialy, the complement fixation test. In this moment we have not specific therapy for this new disease but because its epidemiological importance it must be in all clinical minds.

Adult↗