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J Repérant

Publications and source records attributed to J Repérant.

139 records · Page 8Linked to original sources
PubMedDate not supplied

Colocalization of neurotransmitters in presynaptic boutons of inhibitory synapses in the lamprey spinal cord.

Electron-microscopic immunocytochemical studies were performed to detect GABA and glycine immunoreactivity in presynaptic axon terminals in the central gray matter of the spinal cord of the lamprey Lampetra fluviatilis. The immunopositive presynaptic terminals contacting identified dendrites of motoneurons and unidentified postsynaptic profiles included terminals immunopositive for GABA only (44%) and glycine only (26%), as well as terminals containing GABA and glycine (30%). Glycine-immunopositive presynaptic terminals contained flattened synaptic vesicles. Large synaptic vesicles with dense cores were present along with classical synaptic vesicles in 74% of GABA-immunopositive boutons.

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PubMedDate not supplied

The serotoninergic system of the brain of the viper, Vipera aspis. An immunohistochemical study.

Serotoninergic cell bodies and fibers in the brain of the viper, Vipera aspis, were visualized by immunohistochemistry. Immunoreactive cell bodies were observed in the diencephalic hypothalamic periventricular organ and in the dorsal wall of the infundibular recess, in the nuclei raphe superior and inferior of the midbrain and hindbrain, and to a lesser extent in the nuclei reticularis superior, reticularis inferior and reticularis lateralis. In contrast to other reptilian species, serotoninergic cells were also observed in the central gray matter of the midbrain in the neighbourhood of the nucleus of the trochlear nerve. Immunoreactive fibers are widely distributed throughout the brain of the viper. In the olfactory bulb, fibers were observed in the internal plexiform layer and mitral cell layer. The cerebral cortex contains the highest density of fibers in the dorsal region. The distribution of immunoreactive fibers in the dorsal ventricular ridge is extremely heterogeneous, and five subcomponents of this structure can be distinguished. The majority of diencephalic and mesencephalic structures that contain immunoreactive fibers are also primary visual centres: the nuclei geniculatus lateralis pars dorsalis, the n. posterodorsalis and n. opticus tegmenti, and the optic tectum. Serotoninergic fibers in the nuclei of the oculomotor and motor cranial nerves (III, IV, V, VII, X) are disposed in a tightly woven basket around the non-immunoreactive cell bodies of the motoneurons. These findings, together with the available literature, suggest that the serotoninergic system in snakes is comparable to that in lizards, with a massive ascending projection of fibers from the n. raphe superior to mesencephalic and prosencephalic structures, and a descending projection from the n. raphe inferior to the spinal cord.

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PubMedDate not supplied

Sequential events of degeneration and synaptic remodelling in the viper optic tectum following retinal ablation. A degeneration, radioautographic and immunocytochemical study.

The ultrastructural changes taking place in the retino-recipient layers of the viper optic tectum were examined between 5 and 122 days after retinal ablation. The initial degeneration of retinotectal terminals proceeds at widely different rates and is characterized by a marked degree of polymorphism in which a number of different patterns can be discerned. In the final stages of degeneration, either both the degenerating bouton and the distal portion of the postsynaptic element are engulfed by reactive glia, or, more frequently, only the degenerating terminal is eliminated and the postsynaptic differentiation remains. The free postsynaptic differentiations are reoccupied predominantly by boutons containing pleiomorphic vesicles and which are for the most part gamma-aminobutyric acid (GABA)ergic, thus forming heterologous synapses; less frequently these sites are occupied by boutons of the ipsilateral visual contingent to form homologous synapses. These two processes, both of which depend on terminal axonal sprouting, take place within the first 3 postoperative months. They are followed by a decrease in the number of heterologous synapses and a concurrent increase in the number of homologous synapses newly formed by optic boutons generated by collateral preterminal sprouting of ipsilateral retinotectal fibres. The data suggest that partial deafferentation of the optic tectum induces a transitory GABAergic innervation of free postsynaptic sites prior to the restoration of new retinal synaptic contacts.

Afferent Pathways↗
PubMedDate not supplied

The serotoninergic system of the brain of the lamprey, Lampetra fluviatilis: an evolutionary perspective.

The distribution of serotonin(5HT)-immunoreactive cell bodies, nerve fibers and terminals was investigated by light microscopy in the lamprey Lampetra fluviatilis. Twenty-three distinct groups of 5HT neuronal somata were identified from diencephalic to rhombencephalic levels in the brain. The diencephalon contained a subependymal population of immunoreactive cells in contact with the cerebrospinal fluid (CSF), which could be subdivided into five separate groups situated in the hypothalamus and ventral thalamus; five additional groups of immunoreactive diencephalic neurons, situated in the dorsal thalamus and thalamo-pretectum, which were not in contact with the CSF, were also identified. In the midbrain, in addition to a few labelled neurons in the optic tectum, two structures containing immunoreactive cells were identified in the tegmentum mesencephali. None of these 5HT cells corresponded to the retinopetal neurons which are situated in the same region. A very large number of 5HT neurons were observed in the hindbrain which could be divided into seven groups in the isthmus rhombencephali and a further three in the rhombencephalon proper. Immunoreactive fibers and terminals were widely distributed throughout the neuraxis. In the telencephalon two 5HT fibers assemblies, lateral and medial, could be identified which terminated in both pallial and subpallial structures. The richest serotoninergic innervation in the telencephalon was found in the lateral portion of the primordium hippocampi and the medial part of the corpus striatum. In the diencephalon, the distribution of immunoreactive fibers and terminals was heterogeneous, being most pronounced in the lateral hypothalamic area and in the infundibulum. The densest arborization of fibers in the mesencephalon was found in the stratum fibrosum et cellulare externum of the optic tectum, a major site of retinal projection, and in the nucleus interpeduncularis mesencephali as well as in the oculomotor nuclei. The rhombencephalon is richly endowed with serotoninergic fibers and terminals, many labelled arborizations being found in the nuclei isthmi rhombencephali and around the nucleus motorius nervi trigemini. Comparative analysis of the serotoninergic systems of petromyzontiforms and gnathostomes indicates that the evolution of this system involves a progressive elimination of the rostral immunoreactive cells and an increasing complexity of the caudal population of serotoninergic neurons.

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PubMedDate not supplied

Ultrastructural study of the optic nerve in blind mole-rats (Spalacidae, Spalax).

The optic nerve in two species of subterranean mole-rats (Spalacidae) has been examined at the ultrastructural level. The axial length of the eye and the diameter of the optic nerve are 1.9 mm and 52.5 microns in Spalax leucodon, and 0.7 mm and 80.8 microns in Spalax ehrenbergi, respectively. An anti-glial fibrillary acidic protein postembedding procedure was used to distinguish glial cell processes from axons. In both species, the optic nerve is composed exclusively of unmyelinated axons and a spatial distribution gradient according to the size or the density of fibers is lacking. The optic nerve of S. leucodon contains 1790 fibers ranging in diameter from 0.07-2.30 microns (mean = 0.57 microns), whereas in S. ehrenbergi, only 928 fibers, with diameters of 0.04-1.77 microns (mean = 0.53 microns) are observed. In S. ehrenbergi, a higher proportion of glial tissue is present and the fascicular organization of optic fibers is less obvious. Distribution gradients according to size frequency or density of fibers in the optic nerve are absent in both species. Comparison with other mammals suggests that although ocular regression in microphthalmic species is correlated with a significant decrease in the total number of optic fibers and the relative proportion of myelinated fibers, no difference in the absolute size range of unmyelinated axons is observed. The total absence of myelinated fibers in Spalax may be related to the subcutaneous location of the eyes.(ABSTRACT TRUNCATED AT 250 WORDS)

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PubMedDate not supplied

GABA immunoreactivity in the nucleus isthmo-opticus of the centrifugal visual system in the pigeon: a light and electron microscopic study.

The present study examined GABA immunoreactivity within the retinopetal nucleus isthmo-opticus (NIO) of the pigeon centrifugal visual system (CVS) using light- (immunohistofluorescence, peroxidase anti-peroxidase: PAP) and electron- (postembedding GABA immunogold) microscopic techniques. In some double-labeling experiments, the retrograde transport of the fluorescent dye rhodamine beta-isothiocyanate (RITC) after its intraocular injection was combined with GABA immunohistofluorescence. GABA-immunoreactive (-ir) somata were demonstrated within the neuropilar zone of the NIO adjacent to the centrifugal cell laminae whereas the centrifugal neurons were always immunonegative. A quantitative ultrastructural analysis was performed which distinguished five categories of axon terminal profiles (P1-5) on the basis of various cytological criteria: type of synaptic contact (symmetrical or asymmetrical); shape, size, and density of synaptic vesicles as well as the immunolabeling (positive or negative), size of profile and appearance of hyaloplasm. Numerous GABA-ir afferents to centrifugal neurons via axon terminal types P2a, P2c, and P3 were observed which comprised 47.1% of the total input. Moreover, the data suggest that some of the P2a terminals, which make up 26.4% of the input, stem from the intrinsic GABA-ir interneurons, whereas the latter receive P1, P3, but also P2 terminal input, indicating that interneurons may contact other interneurons via type P2a axon terminals. The results also suggest that the GABA-ir P3 or the immunonegative P1b and P5 axon terminals are of extrinsic origin arising from cells in the optic tectum whereas the P2c and P4 axon terminals are associated with extra-tectal input to the NIO. The GABAergic innervation of centrifugal neurons within the NIO may be the basis for the demonstrated facilitatory effect of the centrifugal output upon ganglion cell responses. This is relevant to hypotheses regarding CVS involvement in attentional mechanisms through selective enhancement of retinal sensitivity depending on the location of meaningful or novel stimuli.

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PubMedDate not supplied

Brain-stem afferents upon retinal projecting isthmo-optic and ectopic neurons of the pigeon centrifugal visual system demonstrated by retrograde transneuronal transport of rhodamine beta-isothiocyanate.

Brain-stem afferents to the n. isthmo-opticus (NIO) and ectopic neurons (EN) of the centrifugal visual system (CVS) were determined in the pigeon using the retrograde transneuronal transport of the fluorescent dye Rhodamine beta-isothiocyanate (RITC) after its intraocular injection. In other experiments, either RITC was injected into various periocular tissues (controls) or the retrograde tracer Fluoro-gold (FG) was injected stereotaxically in the NIO. Following intraocular injections, the RITC retrograde labeling of cell bodies was observed contralaterally in the NIO and EN and transneuronally in layers 9/10 of the optic tectum, area ventralis-Tsai, zona peri-NIII, mesencephalic and pontine reticular formation (PRF), n. linearis caudalis-raphe, and bilaterally within a region referred to as zona peri-n.NVI (Zp-n.NVI) immediately underlying the abducens nerve nucleus. None of the above structures were labeled after RITC periocular injections. The FG labeling revealed that the tectal efferent neurons were mainly medium-sized, multipolar cells whose dendrites extended superficially to retino-recipient tectal layers 6 and 5. Quantitative measurements of the distribution of layers 9/10 RITC-labeled neurons indicated the highest densities to be localized within the ventral tectum corresponding to the representation of the dorsal retina and inferior visual field. We suggest that visual and nonvisual brain-stem afferents upon NIO and EN may play a role in the proposed mechanism of the avian CVS in attention, ground-feeding behavior, and modulation of retinal sensitivity.

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PubMedDate not supplied

[Experimental study of visual projection specificity in microphthalm and monophthalm embryos and adults of Gallus domesticus L].

Research was carried out into the visual projections of embryos and chickens of Gallus domesticus L. who had undergone early optic vesicle removal and into microphthalmy or monophthalmy with ipsilateral optic fibres resulting from such removal. The architectonics of primary visual centres (nuclei ectomamillaris, geniculatus lateralis, lateralis anterior superficialis synencephali, griseus tectalis and the tectum opticum superficiale) and of the isthmo-opticus nucleus were compared with the architectonics of the same centres in anophthalms. From this research it can be seen that:--1. Optic fibres coming from limited ocular formation in microphthalms can reach the ectomamillaris nucleus in most cases and sustain existence; they may reach the tectum opticum without playing a qualitatively discernable morphogenetic role and act upon the isthmo-opticus nucleus. For these microphthalms, the nuclei lateralis anterior, geniculatus lateralis, superficialis synencephali and griseus tectalis are comparable to those of anophthalms. 2. Ipsilateral optic fibers can develop and show the same specificity and morphogenetic function as the microphthalms' optic fibres. 3. After hatching, some anophthalms shows an isthmo-opticus nucleus with scores of neurons. In general, observations during this research have shown that the specificity of microphthalms' optic fibres and ipsilateral optic fibres remain strictly the same whatever the operation under consideration.

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PubMedDate not supplied

[GABA immunoreactivity in the main olfactory bulb of the frog Rana temporaria].

Immunoreactivity for gamma-aminobutyric acid (GABA) was localized at the light microscopic level in the main olfactory bulb (MOB) of the frog, Rana temporaria. By means of free-floating peroxidase-antiperoxidase immunocytochemical technique, GABA was found in a large number of neurons in the granular cell layer, in a few small somata in the mitral cell layer and in two different types of cell somata in the glomerular layer. Individual GABA-immunopositive cells were found in the olfactory nerve layer. GABA immunostaining was also localized in cell processes and fiber fragments. There were many immunoreactive puncta in all layers of the MOB. GABA-positive punctate structures often outlined immunonegative cells in the mitral cell and glomerular layers. Rounded tightly packed groups of immunoreactive puncta were found only along ventral border of the glomerular layer. The results are discussed in comparison with data obtained on mammalian MOB in terms of MOB functional organization.

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