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J Reuter

Publications and source records attributed to J Reuter.

30 records · Page 2Linked to original sources
PubMed1992

Multiple drug resistance gene expression in human renal cell cancer is associated with the histologic subtype.

Overexpression of p170 glycoprotein, the product of the multiple drug resistance (mdr) gene, has been associated with resistance to various cytotoxic drugs used in the treatment of human neoplasms. Normal renal epithelial cells express p170 as a function of their secretory capacity. Because renal cell carcinomas (RCC) respond poorly to chemotherapeutic regimens, p170 expression was studied in primary RCC. Such expression was measured in 40 human RCC and normal kidney tissues using immunohistochemical staining with the monoclonal antibody C-219. Staining intensities of the whole tumor and of different areas of the cryostat sections were transformed into digital numbers using an algorithm designed for this purpose. In most tumors, an inhomogeneous staining pattern and a correlation between grade of differentiation and C-219 immunoreactivity was observed. A comparison of the tumors according to their histopathologic subtypes showed clear differences. The means (range) of the staining intensities of the different types of RCC: clear cell carcinoma Grade 1 (n = 3), 2.0 (2.0 to 2.0); clear cell carcinoma Grade 2 (n = 19), 0.8 (0.0 to 2.9); clear cell carcinoma Grade 3 (n = 5), 0.1 (0.0 to 0.2); tubular carcinoma (n = 4), 2.0 (2.0 to 3.0); anaplastic carcinoma (n = 8), 0.05 (0.0 to 0.2); oncocytoma (n = 1), 0.0 (0.0 to 0.0); and normal kidney (n = 40), 0.5 (0.0 to 2.0). The differences between anaplastic, clear cell, and tubular carcinoma were significant (P less than 0.001 by Kruskal-Wallis test). In addition, the difference between the three subgroups of clear cell carcinoma was significant (P less than 0.01). It was concluded that the histopathologic subtypes of RCC correlate with the degree of mdr gene expression, as determined by staining with the C-219 monoclonal antibody.

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMed1988

Folinic acid effect on 5-fluorouracil kinetics in vivo.

Synergy of folinic acid and FU has been shown in several experimental systems. In the present study we examined the kinetics of concurrent administration of folinic acid on the kinetics of FU in an in vivo model. Folinic acid significantly increased the amount of FU bound to the TS complex. In the sequential combination of MTX and FU folinic acid had no additional effect. The amount of FU incorporated into RNA was not affected in any of the treatment groups. The results indicate that folinic acid may improve FU cytotoxicity by increased FU binding to TS but has no additional effect on the synergistic sequential MTX-FU combination.

Animals↗
PubMed1985

Factors that affect the HMO enrollment decision: a tale of two cities.

Although there is agreement that utilization patterns in HMOs differ from those in non-HMO systems, there is less agreement on how and why these differences are achieved. Selection bias is one frequently offered explanation. In an attempt to identify those individual and site-specific factors that help determine employees' choice of coverage, we developed and tested a logistic model of the enrollment decision. We used both survey data and claims data to study the decision to enroll in either an HMO or a traditional indemnity plan among state employees in Tallahassee, Florida, and Salt Lake City, Utah. We found that many factors affected the decision--factors that could be altered by an HMO or insurance company to correct selection bias if and when it is encountered.

Adult↗
PubMed1984

Validity of caregiver information on the developmental status of severely brain-damaged young children.

Two studies were conducted to examine the validity of caregiver information on the developmental status of severely brain-damaged young children. First, developmental information on 106 severely brain-damaged young children, obtained from a caregiver-completed behavior inventory--the Kent Infant Development Scale, was compared to that obtained from the Bayley Scales of Infant Development (the Bayley Scales). Developmental age estimates from both tests were highly correlated and similar. Results suggest that it is possible to substitute the Kent Infant Development Scale for the Bayley Scales when measuring the developmental status of severely brain-damaged young children. In a second study examining the differential validity of caregivers' reports, we found that mothers' Kent Infant Development Scales yielded higher developmental age estimates than did teachers' Kent Infant Development Scales.

Brain Damage, Chronic↗
PubMed1980

Social milieu of a residential treatment center for severely or profoundly handicapped young children.

Severely or profoundly handicapped young children who lived in a residential treatment center were subjects in a modified time-sampled observation study of social behavior, state, and physical context. The study was conducted for 3 successive years. Although system-level changes in the ecology of the institution over the 3 years did not appear to affect the social-interaction indices, the developmental ages of the children were positively related to social opportunities and social behavior.

Child Development↗
PubMed1976

Grief response of parents after referral of the critically ill newborn to a regional center.

Attitudes, feelings, and behavior were studied in 101 mother-father pairs whose critically ill newborn infants survived after referral from the hospital of birth to a regional neonatal intensive-care unit for special care. As measured by an anticipatory-grief score, most parents experienced grief reactions similar to those whose infants do not survive the newborn period. The level of anticipatory grief did not appear to be associated with severity of infant illness as determined by the need for respirator therapy or major surgical procedures. The fathers reported drastic alteration in daily activity while wife and infant were hospitalized in separate facilities, and they assumed a central role in maintaining family stability during the crisis. These data suggest that an organized family-support program could play a prominent part in maintaining family stability during the delivery of newborn intensive care.

Adult↗
PubMed1976

The subacute and chronic toxicity of cetyltrimethylammonium bromide (CTAB), a cationic surfactant, in the rat.

Cetyltrimethylammonium bromide (CTAB), a cationic surfactant, was given to male and female Sprague-Dawley rats in drinking water in dosages of approximately 10, 20, and 45 mg/kg/day for 1 year. The surfactant was well tolerated at the two lowest dose levels. At the highest dose level a reduction in body weight was observed. In males the body weight remained reduced throughout the study, but in females the body weight was reduced only during the first 2 months of the study. It was found that food conversion was less efficient in male rats during the first 7 weeks of the study. No compound-related gross pathologic changes were seen on autopsy and no microscopic alterations were found in the wall of stomach and small intestine of treated animals.

Animals↗
PubMed1976

Some aspects of the growth of rats.

The first quantitatively significant phase of growth for the rats under investigation was finished at body weights of 260 g in females and 410 g in males. During this phase only growth will influence the energy requirements of the rat. The sex-dependent difference in size will be discussed; it is probably caused by the fact that the period of marked cell division activity lasts slightly more than one week longer in male than in female rats.

Age Factors↗
PubMedDate not supplied

Academic health centers in competitive markets.

Academic health center (AHC) hospitals and other major teaching hospitals have funded a portion of their academic missions through patient care revenues. Using all-payer state discharge data, this DataWatch presents information on how these institutions are being affected by market changes. Although AHCs are not as successful as other hospitals are in attracting managed care patients, competitive pressures had not eroded AHCs' financial status as of 1994. However, increasing enrollment in managed care and potential changes in both Medicare and Medicaid suggest that pressure on the financing of these institutions' social missions will continue to grow over time.

Academic Medical Centers↗
PubMedDate not supplied

Assessment of drug-induced dysregulations among seven resistance-associated genes in human tumour cell lines.

Drug-resistance in cell lines and in malignant human tumours is associated with dysregulation of several genes including mdr1, MRP1, GST-pi, bcl-2, DNA topoisomerase II alpha and beta, and thymidine kinase I. mRNA expression was evaluated by quantitative RT-PCR coupled with HPLC in three human tumour cell lines and drug-resistant (DR)-sublines. DR sublines from RPMI-8226 and KB cells specifically overexpressed the mdr1 gene without major changes observed in other putative DR-associated genes. In contrast, the DR-H69 cells exhibited a 34-fold overexpression of the MRP gene accompanied by significant down-regulation of both DNA topoisomerase II alpha and bcl-2 mRNA gene expression, by factors of 43 and 13 respectively. These results demonstrate the concomitant down regulation of topoisomerase II alpha and bcl-2 genes in response to DR. Furthermore, differential patterns of gene dysregulations appear to vary depending upon both the drug used to select resistance and cellular origin.

DNA Topoisomerases, Type II↗