Nucleoside utilization by S and G1 cells.
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J Rigau-Lloveras
Publications and source records attributed to J Rigau-Lloveras.
[Method for selecting cells during various phases of the mitotic cycle].
Exponentially growing L5178Y cells in suspension culture were separated according to their position in the cell cycle on the basis of their volume with a velocity sedimentation method in which a linear and continuous ficoll gradient was used. Highly purified populations of G1 and S cells were obtained, containing about 90% G1 phase cells and 80% S phase cells. The method is rapid and a larger number of cells can be easily processed with no loss of viability.
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