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Biomedical subjects

J Rippon

Publications and source records attributed to J Rippon.

3 recordsLinked to original sources

Non-aspergillus aspergilloma.

A case of lung cavity with fungus ball is reported in a patient subsequent to the treatment of tuberculosis for a year. Fungal serologic titers for histoplasmin, blastomycin, coccidiodin, and aspergillin were negative. Surgical resection of the cavity and to be done because of episodes of hemoptysis. Numerous hyphae formed into a compact mass consistent with aspergilloma were seen in the cavity. The cultures for fungi were negative on material from the surgical specimen. Immunodiffusion studies of sera obtained prior to surgery were positive for Pseudallescheria boydii and very informative in assessing the correct morphologic characteristics of the organism.

Ascomycota↗

The effect of parenteral alimentation fluid, undiluted with saline or fresh sera, on the growth of Candida albicans in vitro at 37 degrees C.

Parenteral alimentation is often complicated by Candida albicans infection which may be fatal. This study investigated the effect of alimentation fluid (Aminosol) on C. albicans' growth in vitro. It was found that concentrated Aminosol (1400 millisomoles) maintained C. albicans in a viable state but inhibited replication. Dilution of alimentation fluid to physiological concentrations (300 milliosmoles) with either saline or aged pooled normal sera promoted in vitro growth of C. albicans which was equivalent to that obtained in BHI broth and was slightly less than that obtained in Sabouraud's broth. The effects of fresh sera with full complement activity were also investigated. In fresh sera appropriately diluted with physiological saline, some clumping of the yeasts was observed and all formed germ tubes. Growth as defined by budding or the formation of hyphae was inhibited. When Aminosol was diluted to 300 milliosomoles with fresh sera, all yeasts were noted to be in clumps with germ tubes as well as continually growing hyphae. Growth was approximately equal to that seen in Aminosol similarly diluted with saline.

Blood Proteins↗