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Biomedical subjects

J Robin

Publications and source records attributed to J Robin.

At least 91 records · Page 5Linked to original sources
PubMed1980

Resistance of herpes channel catfish virus (HCCV) to temperature, pH, salinity and ultraviolet irradiation.

The effect of temperature, pH, salinity and ultraviolet irradiation on the infectivity of Herpes Channel Catfish Virus (HCCV) has been determined. It has been shown that the pH and the culture medium is an important factor in maintaining virus infectivity. The temperatures higher than 4 degrees C rapidly inactivate the virus while this one can be grown successfully after several months of storage at -75 degrees C and at -20 degrees C. In common with other herpesviruses, HCCV is quickly inactivated by UV irradiation. The signification of these findings on the mode of viral infection and of viral harvesting, disinfection measures and marine farming of Ictalurids is important.

Animals↗
PubMed1978

Purification of Channel Catfish virus, a fish herpesvirus.

Channel Catfish virus was highly purified from host cells by precipitation with PEG-6000 and isopycnic centrifugation in a metrizamide gradient. As calculated from reconstruction experiments, only 0.09 and 0.05% respectively of the host DNA and the host proteins were recovered at the position of the viral band. The final recovery of infectivity was about 30%. Electron microscopy showed mostly intact viral particles.

Animals↗
PubMed1977

Purification and biochemical properties of Egtved viral RNA.

32P-labeled Egtved virus RNA was released from highly purified virus by phenol-SDS extraction. The single-stranded nature of the RNA was demonstrated by (1) its buoyant density of 1.69 g/cm3 in Cs2-SO4, (2) its susceptibility to digestion by pancreatic ribonuclease in either 1 X SSC or 0.01 X SSC (standard saline citrate), (3) its base composition (29.3% C, 23.6% A, 14.5% U. 32.6% G). This Egtved virus is different from the other rhabdoviruses since the base composition of its genomic RNA is lower in its composition of A + U. Such a result could have possible taxonomic implications concerning the orignin and evolution of Egtved Virus relative to the other known rhabdoviruses.

Adenine↗
PubMed1975

[Double blind test with a protective ointment (Ivosin) on the hands of cement workers].

56 builder's labourers manipulating lime cement applied a protective cream on their hands and 57 used a placebo in a double blind test of two months' duration. The labourers, the doctors who surveyed the condition of their hands, and the statistician who analyzed the results knew only that one cream had a blue label, and the other a green one. Before and after the test, the presence or the absence of certain symptoms on the hands were objectively examined, while the subjective opinion of the labourers was registered at the end of the test. As usual in tests of that kind, the great majority of the labourers (more than 75%) were satisfied, 62% of them acknowledged an improvement. That percentage justified a comparative double-blind study that revealed a much higher quota (86% of the test persons) of satisfaction among the labourers treated with Ivosin than in the placebo group (66%). That difference was statistically significant. On the other hand, no objective difference could be revealed between the two groups. It was impossible to discover among the symptoms studied, any objective cause of that subjective difference.

Anion Exchange Resins↗
PubMed1973

Low-molecular-weight RNAs of murine sarcoma virus: comparative studies of free and 70S RNA-associated components.

Several low-molecular-weight RNAs were released from mouse leukemia sarcoma virus 70S RNA under conditions of thermal denaturation. One of them, the 70S-associated 8S RNA, exhibits a number of structural properties characteristic of a previously described free viral 8S RNA. This similarity was revealed by polyacrylamide gel electrophoresis and nucleotide composition.

Centrifugation, Density Gradient↗