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J Robles

Publications and source records attributed to J Robles.

At least 19 recordsLinked to original sources
PubMed2005

Specific substitutions in the echinocandin target Fks1p account for reduced susceptibility of rare laboratory and clinical Candida sp. isolates.

An association between reduced susceptibility to echinocandins and changes in the 1,3-beta-d-glucan synthase (GS) subunit Fks1p was investigated. Specific mutations in fks1 genes from Saccharomyces cerevisiae and Candida albicans mutants are described that are necessary and sufficient for reduced susceptibility to the echinocandin drug caspofungin. One group of amino acid changes in ScFks1p, ScFks2p, and CaFks1p defines a conserved region (Phe 641 to Asp 648 of CaFks1p) in the Fks1 family of proteins. The relationship between several of these fks1 mutations and the phenotype of reduced caspofungin susceptibility was confirmed using site-directed mutagenesis or integrative transformation. Glucan synthase activity from these mutants was less susceptible to caspofungin inhibition, and heterozygous and homozygous Cafks1 C. albicans mutants could be distinguished based on the shape of inhibition curves. The C. albicans mutants were less susceptible to caspofungin than wild-type strains in a murine model of disseminated candidiasis. Five Candida isolates with reduced susceptibility to caspofungin were recovered from three patients enrolled in a clinical trial. Four C. albicans strains showed amino acid changes at Ser 645 of CaFks1p, while a single Candida krusei isolate had a deduced R1361G substitution. The clinical C. albicans mutants were less susceptible to caspofungin in the disseminated candidiasis model, and GS inhibition profiles and DNA sequence analyses were consistent with a homozygous fks1 mutation. Our results indicate that substitutions in the Fks1p subunit of GS are sufficient to confer reduced susceptibility to echinocandins in S. cerevisiae and the pathogens C. albicans and C. krusei.

Amino Acid Substitution↗
PubMed2002

Retro-esophageal subclavian artery: a case report.

This case report presents the finding of an aberrant right subclavian artery during the routine dissection of 230 human adults specimens. The case which we describe appeared in an 82-year-old male donor, in whom the right retro-esophageal subclavian artery was observed as the last branch of the aortic arch. The rate of occurrence of this variation is stated to be less than 1%. This variation is described in detail; embryology and anatomy are reviewed and we discuss it in relation to other cases. The aortic arch was dissected, and its branches were described as regards their relations, length and diameter.

Aged↗
PubMed1995

Solid-phase synthesis of a nucleopeptide from the linking site of adenovirus-2 nucleoprotein, -Ser(p5'CATCAT)-Gly-Asp-. Convergent versus stepwise strategy.

The synthesis of a nucleopeptide with the sequence -Ser(p5'CATCAT)-Gly-Asp- has been undertaken by either convergent or stepwise solid-phase strategies, both of which use base-labile permanent protecting groups. The coupling of phosphitylated protected peptides onto oligonucleotide-resins did not afford the desired nucleopeptide, which was nevertheless obtained after oligonucleotide elongation at the hydroxyl group of the resin-bound peptide and deprotection under mild basic conditions. A preliminary study on the stability of different nucleopeptides to bases is also reported.

Adenoviruses, Human↗
PubMed1995

[High-flow priapism].

OBJECTIVES: A case of traumatic high flow priapism that had been successfully treated by selective embolization of the internal pudendal artery is described. METHODS: High flow priapism was diagnosed in a patient who presented with painless prolonged erection. He had fallen down from a horse twelve days before. The intracavernous blood gas analysis had arterial characteristics and erection did not subside with conservative management. Selective embolization of the internal pudendal artery was performed after visualization of an arteriocavernous fistula. RESULTS: Priapism disappeared just after the procedure and sexual potency was preserved. CONCLUSIONS: Selective embolization of the internal pudendal artery is an appropriate treatment for traumatic high flow priapism.

Adult↗
PubMed1995

[Epithelioid hemangioendothelioma: a rare indication for liver transplantation?].

A clinical case of epithelioid hemangioendothelioma without extrahepatic involvement treated with liver transplantation is presented. The patient remains alive 42 months thereafter without tumor recurrence. A review of cases reported to date was carried out with special reference being made to the therapy undertaken and the follow-up.

Adult↗
PubMed1994

Preparation of an aspartic acid-containing protected peptide. Alternatives for overcoming a side reaction during HF treatment.

Treatment of the peptide-resin Ac-Asn-Ser(Bzl)-Gly-Asp(OFm)-pMeBHA with HF/anisole cleaves the peptide-resin bond and removes the benzyl group, as expected, but also yields an impurity (ca. 25%) in which the benzyl group is linked to the fluorenylmethyl protecting group. Separation of the desired peptide from the impurity can be accomplished by reversed-phase medium-pressure liquid chromatography. The side reaction does not take place when the gamma-carboxyl of aspartic acid is protected with the 2-(4-acetyl-2-nitrophenyl)ethyl group.

Alkylation↗
PubMed1993

PCP: a review of synthetic methods for forensic clandestine investigation.

A review of the synthetic routes to phencyclidine (PCP, 1-(1-phenylcyclohexyl)piperidine) available in the open literature is presented. The emphasis herein is directed toward the forensic investigation of clandestine PCP laboratories. Six published synthetic routes to PCP/analogs are discussed. Each method is rated for overall yield, degree of difficulty and potential hazard, in order to assist the forensic chemist in evaluation of a particular clandestine operation. One clandestine recipe is illustrated and discussed.

Forensic Medicine↗
PubMed1988

Bacillus Calmette-Guérin immunotherapy in ovarian cancer.

Forty-six advanced ovarian cancer patients treated with conventional modalities with the addition of bacillus Calmette-Guérin (BCG) immunotherapy showed prolonged survival when compared to controls not given BCG. Although the data suggest enhancement of survival with the addition of BCG to conventional treatment, the fact remains that disease recurrence ultimately claims the lives of most of these patients. Nonetheless, patients are surviving longer in the face of advanced disease.

BCG Vaccine↗