A model to study development and immune function in the human small intestine.
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Biomedical subjects
J S Friedberg
Publications and source records attributed to J S Friedberg.
Hepatocytes from rat liver perfusions. Physicochemical effects on polyribosome size.
Hepatocytes have been used extensively both in vitro and in vivo for a wide variety of studies. Although isolated hepatocytes appear similar to their in vivo counterparts, they are different in some respects. One aspect in which they differ is their ability to translate proteins. The isolation process appears to inflict sufficient damage to reduce the average polyribosome size within the isolated hepatocytes and this size reduction correlates with a lower rate of protein production. Two methods of liver perfusion that better maintain the normal distribution of polyribosomes are described in this report. It is clear from these studies that new isolation methods that result in high yields of viable hepatocytes that function maximally are needed.
Animals↗
A quantitative model of invasive Pseudomonas infection in burn injury.
To evaluate newer therapies for wound infections, it becomes necessary to quantify bacteria that invade from the infected wounds into the adjacent tissues. For example, antibody-targeted photolysis targets the invasive Pseudomonas with antibodies carrying photochemical dyes. A full-thickness burn wound was infected with Pseudomonas aeruginosa with a modification of previous methods. In mice, a skin fold was elevated, and two preheated brass blocks at 92 degrees to 95 degrees C were applied for 5 seconds, producing a 5% total body surface area injury with discrete margins. The eschars were immediately inoculated with Pseudomonas. Survival at 10 days was 100% with burn injury alone and 60% with infected burns. Pseudomonas (10(8)/gm) were recovered from the unburned muscle by 24 hours. The method produced uniform and reproducible quantitative bacteriology within the muscle immediately beneath the burn injury (SL < 0.05). Quantitative comparisons can be used to determine the effectiveness of newer modalities to control Pseudomonas burn wound infections.
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