Infectivity of Leishmania donovani primary culture promastigotes for golden hamsters. Short communication.
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Animals↗
Biomedical subjects
J S Keithly
Publications and source records attributed to J S Keithly.
Infectivity of Leishmania donovani amastigotes and promastigotes for golden hamsters.
Intracardial injection of hamsters with from 5 to 114 million amastigotes or promastigotes of Leishmania donovani and screening of the 8th-day liver impression smears, provides a rapid and reproducible method for assaying infectivity. Amastigotes are at least 10X more infective than promastigotes, and log-phase promastigotes act as a single infective population for hamsters.
Animals↗
The beta tubulin gene of Eimeria tenella.
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Amino Acid Sequence↗
Ornithine decarboxylase and trypanothione reductase genes in Leishmania braziliensis guyanensis.
Ornithine decarboxylase and trypanothione reductase are the key enzymes in polyamine and trypanothione metabolism in kinetoplastids. Using a heterologous Trypanosoma brucei brucei probe for ornithine decarboxylase and a mixed synthetic probe of 29 oligonucleotides for trypanothione reductase, we have detected the putative genes for these enzymes by Southern blot hybridization using genomic DNA of Leishmania braziliensis guyanensis MHOM/SR/80/CUMC 1. The trypanothione reductase probe was constructed both from the conserved codon usage of the redox active site for other flavin oxidoreductases over a wide evolutionary scale, and the preferred codon usage for other genes in species of Leishmania.
Amino Acid Sequence↗
Cryptosporidium parvum: polyamine biosynthesis from agmatine.
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Acetyltransferases↗
Preliminary evidence for a mitochondrion in Cryptosporidium parvum: phylogenetic and therapeutic implications.
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Adenylate Kinase↗