PubMed Health⌕ Search

Biomedical subjects

J S Newman

Publications and source records attributed to J S Newman.

10 recordsLinked to original sources
PubMed1995

Prostate cancer: diagnosis with color Doppler sonography with histologic correlation of each biopsy site.

PURPOSE: To correlate the findings at prostate color Doppler sonography (CDS) with those of site-specific transrectal core biopsy. MATERIALS AND METHODS: Forty-three patients underwent prostate transrectal ultrasonography (US) and biopsy. CDS was performed at all biopsy sites before US-guided core biopsy. Vascularity at CDS was prospectively graded on a scale of 0-2 (0 = no visible peripheral zone [PZ] flow, 2 = markedly increased PZ vascularity). CDS results were correlated with histologic findings from 220 separate biopsy sites that included 27 focal lesions. RESULTS: Of 34 grade 2 biopsy sites, 21 revealed carcinoma, eight revealed prostatitis, and five were negative. CDS depicted at least one focus of carcinoma in seven patients with no gray-scale abnormality. CDS had a sensitivity of 49%, specificity of 93%, and positive predictive value of 62%. CONCLUSION: Focal PZ hypervascularity at CDS is associated with an increased likelihood of prostate cancer or inflammation at biopsy, often without a focal gray-scale abnormality. CDS may help identify an appropriate site for biopsy. A negative CDS scan, however, should not preclude biopsy, as CDS has a limited sensitivity in the detection of all sites of cancer.

Aged↗
PubMed1991

Stability and structure of three-way DNA junctions containing unpaired nucleotides.

Non-paired nucleotides stabilize the formation of three-way helical DNA junctions. Two or more unpaired nucleotides located in the junction region enable oligomers ten to fifteen nucleotides long to assemble, forming conformationally homogeneous junctions, as judged by native gel electrophoresis. The unpaired bases can be present on the same strand or on two different strands. Up to five extra bases on one strand have been tested and found to produce stable junctions. The formation of stable structures is favored by the presence of a divalent cation such as magnesium and by high monovalent salt concentration. The order-disorder transition of representative three-way junctions was monitored optically in the ultraviolet and analyzed to quantify thermodynamically the stabilization provided by unpaired bases in the junction region. We report the first measurements of the thermodynamics of adding an unpaired nucleotide to a nucleic acid three-way junction. We find that delta delta G degrees (37 degrees C) = +0.5 kcal/mol for increasing the number of unpaired adenosines from two to three. Three-way junctions having reporter arms 40 base-pairs long were also prepared. Each of the three reporter arms contained a unique restriction site 15 base-pairs from the junction. Asymmetric complexes produced by selectively cleaving each arm were analyzed on native gels. Cleavage of the double helical arm opposite the strand having the two extra adenosines resulted in a complex that migrated more slowly than complexes produced by cleavage at either of the other two arms. It is likely that the strand containing the unpaired adenosines is kinked at an acute angle, forming a Y-shaped, rather than a T-shaped junction.

Base Sequence↗
PubMed1989

Venesection as a rare form of self-mutilation.

This case history records a rare form of self-mutilation, venesection. Underlying psychopathological mechanisms are presented, and contrasted with findings in the only other similar case reported in the English literature. The complex etiology of self-mutilating is reviewed with reference to this case report.

Adult↗
PubMed1985

Factors affecting desired family size among preliterate New Guinea mothers.

A random sample of 331 Enga mothers in Papua New Guinea perceived that an average of 5.96 live births (S.D. = 1.88) were needed to achieve their mean desired completed family size (DFS) of 4.65 children (S.D. = 1.32). The mean of the personal child mortality rates projected by the individual mothers. 194/1000, is very close to the rate of 198/1000 (224 deaths among 1134 live births) experienced by the women as a group and the 177/1000 documented in a 1972 prospective study in the area. This suggests that as a group preliterate women may possess an accurate estimate of prevailing child mortality rates. Considerable interest in family planning was shown. However for cultural or linguistic reasons, the majority (except in the case of the pill and tubal ligation) expressed no opinions about their readiness to use specific modern methods. The mean parity of 43 women seeking tubal ligation was 5.98 (S.D. = 1.81). An integrated maternal health and family planning program focusing on the health benefits to mother and child of the current 3-4-year birth interval seems indicated.

Adult↗
PubMed1977

Inactivation of polioviruses and coxsackieviruses in surface water.

Inactivation rates of polioviruses 1 and 3 and coxsackieviruses A-13 and B-1 were determined in situ in the Rio Grande in southern New Mexico, using membrane dialysis chambers. Inactivation of the viruses was exponential, and the rates of inactivation were apparently affected principally by the water temperature. Stability of the viruses in river water differed, with poliovirus 1 and coxsackie B-1 being most stable. Typically 1-log reductions of infectivity at water temperatures ranging between 23 and 27 degrees C required 25 h for poliovirus 1, 19 h for poliovirus 3, and 7 h for coxsackie virus A-13. At water temperatures of 4 to 8 degrees C, the log reduction times for poliovirus 1 and coxsackievirus B-1 were 46 and 58 h, respectively. Results obtained with labeled poliovirus 1 and coxsackievirus B-1 and with infectious ribonucleic acid indicate that inactivation was due to damage to viral ribonucleic acid. Virus-inactivation rates were also affected by heat sterilization of river water, indicating the presence of a heat-labile or volatile inactivating factor. The inactivating factor in Rio Grande water was apparently present at a constant concentration over a 1-year period.

Enterovirus↗
PubMed1976

Interactions between health and population.

Both health and population have multiple direct and indirect, as well as positive and negative, influences on each other and on the development process. There is much evidence to show that high fertility produces multiple health hazards and that family planning is one of the most effective health measures available. Conversely, there are also multiple ways in which health influences fertility. Whereas health services resulting in reduced morbidity and mortality have often been considered a major cause of population growth, the authors argue that health programs can also contribute to a more rapid decline in birth rates by facilitating the increased practice of family planning. Evidence is reviewed to support the position that family planning services can be more effective, efficient, and acceptable when combined with maternal and child health and nutrition programs.

Child↗
PubMed1975

Gas chromatographic presumptive test for coliform bacteria in water.

A gas chromatographic procedure which shows promise as a presumptive test for coliform bacteria in water is described. Total coliform bacteria concentrations were determined from the incubation times at 37 C required for ethanol to be produced. Fecal coliform densities were determined in a similar manner at 44.5 C. The culture medium was filter sterilized M-9 salts supplemented with 1% lactose, 0.1% Casamino Acids, and 0.1% yeast extract. Best results were obtained when the initial total coliform concentrations were 5 per ml or higher and when fecal coliform concentrations were 50 per ml or higher. Minimum detection times at these concentrations were 9 and 12 h, respectively.

Chromatography, Gas↗