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J S Nishimura

Publications and source records attributed to J S Nishimura.

51 records · Page 3Linked to original sources
PubMed1972

Phenoxazinone synthetase from Streptomyces antibioticus: multiple activities of the enzyme.

A procedure for the preparation of relatively large quantities of highly purified phenoxazinone synthetase from Streptomyces antibioticus is described. Enzyme preparations consisted of multiple forms, as determined by polyacrylamide gel electrophoresis. Each of the electrophoretically separable forms catalyzed the oxidation of catechols, ferrocyanide, and ethylenic thiols, in addition to o-aminophenols.

Catechols↗
PubMed1969

Biosynthesis of the actinomycin chromophore: incorporation of 3-hydroxy-4-methylanthranilic acid into actinomycins by Streptomyces antibioticus.

Actinomycin synthesis by washed mycelia of Streptomyces antibioticus has been conducted in the presence of 3-hydroxy-4-methylanthranilate-(carboxyl-(14)C). Incorporation of this compound into actinomycins has been observed, which constitutes further evidence that 3-hydroxy-4-methylanthranilate is an intermediate in actinomycin biosynthesis. The position of the incorporated label has been determined to be within the actinomycin chromophore, and the label appears to be equally distributed between both halves of the chromophore. Incidental to these findings was the observation that the (14)C-labeled actinomycins were subject to rapid reabsorption by the organism with actinomycin V taken up preferentially to actinomycin IV.

Carbon Isotopes↗