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J S Son

Publications and source records attributed to J S Son.

3 recordsLinked to original sources
PubMed1998

Role of jasmonic acid in biotransformation of (--)-isopiperitenone in suspension cell culture of Mentha piperita.

The role of jasmonic acid was studied in biotransformation of (--)-isopiperitenone to (--)-7-hydroxyisopiperitenone using alpha suspension cells were treated with (--)-isopiperitenone, mRNA of a cytochrome P450 was induced in a similar time-course pattern as the biotransformation shown in a previous study (Park et al., 1997). The induction of P450 mRNA and the biotransformation of (--)-isopiperitenone were increased by methyl jasmonate, but decreased by salicylhydroxamic acid, and inhibitor of jasmonic acid synthesis. These results suggest that the biotransformation involves the induction of P450 which is mediated by jasmonic acid as a signaling molecule.

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PubMedDate not supplied

Cryopreservation of Taxus chinensis suspension cell cultures.

A simple cryopreservation method for suspension cells of Taxus chinensis was established. In this procedure 7 days old suspension cells were used without any pre-culture treatment. At first, cells were incubated in cryoprotectant solution (0.5M DMSO and 0.5M glycerol) on ice for 30 min and then frozen at a cooling rate of 1 degree C/min to -40 degrees C prior to immersion in liquid nitrogen. The average viability of frozen-thawed cells was between 30 to 40%. The recovery of cryopreserved cells in liquid nitrogen for 1 month was accomplished. After rapid thawing, cells were transferred to solid medium and cultivated for 4-6 weeks. The treatment of trehalose as a cryoprotectant enhanced re-growth of frozen-thawed cells. The stable maintenance of paclitaxel biosynthetic ability in cryopreserved cells was confirmed by comparing with that of regularly sub-cultured suspension cells.

Cell Survival↗