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J S Wallace

Publications and source records attributed to J S Wallace.

17 recordsLinked to original sources

The sharing of water between society and ecosystems: from conflict to catchment-based co-management.

Human uses of freshwater resources are increasing rapidly as the world population rises. As this happens, less water is left to support aquatic and associated ecosystems. To minimize future human water shortages and undesirable environmental impacts, more equitable sharing of water resources between society and nature is required. This will require physical quantities and social values to be placed on both human and aquatic ecosystem requirements. Current water valuation systems are dominated by economic values and this paper illustrates new quantification and valuation methods that take more account of human well-being and environmental impacts. The key to the effective implementation of these more equitable water allocation methods is the use of catchment-based integrated water resources management. This holistic framework makes it possible for human and ecosystem water requirements and the interactions between them to be better understood. This knowledge provides the foundation for incorporating relevant social factors so that water policies and laws can be developed to make best use of limited water resources. Catchment-based co-management can therefore help to ensure more effective sharing of water between people and nature.

Conservation of Natural Resources↗

Intermittent shedding of thermophilic campylobacters by sheep at pasture.

The rates at which sheep on different types of pasture shed campylobacters in their faeces were measured over 12 months. Overall, shedding of campylobacters at pasture was between a third and a half of the carriage rate (92%) of the intestines of sheep at slaughter. Shedding was highest during saltmarsh grazing, followed by upland fell and farm grazing. The rate of shedding varied at different times of the year, with the highest rates (100%) coinciding with lambing, weaning, and movement onto new pasture. The lowest rates (0%) occurred when sheep were fed on hay and silage. On the farm, low rates occurred during the whole of gestation, both when the sheep were indoors and outdoors. Campylobacter jejuni was the main species isolated and survived for up to 4 d in sheep faeces. Lambs became colonized by Campylobacter within 1-5 d of being born. Ewes, which were not shedding campylobacters prior to lambing, started to shed after lambing, and ewes which were shedding low numbers of Campylobacter before lambing, increased the numbers of bacteria being shed after lambing.

Animal Feed↗

Seasonal variation of thermophilic campylobacters in lambs at slaughter.

A survey of the isolation rate and population size of thermophilic campylobacters in lambs at slaughter was carried out to determine the seasonal variation of thermophilic campylobacters in ovine hosts. Isolation rates determined by enrichment methods were always higher than those using direct plating onto selective agar and showed that Campylobacter could be isolated from 91.7% (n = 360) of samples from the small intestine of the lambs. Enumerations (MPN), done monthly over a 2-year period, averaged 4.00 log 10 (n = 1080, S.D. 0.16) campylobacters g-1 fresh weight (fw) intestinal contents with some samples giving values higher than 7 log MPN gfw-1. These results show that the prevalence of thermophilic campylobacters in sheep intended for slaughter is much higher than previously reported. Statistical analyses showed that there was a significant seasonal periodicity in the Campylobacter populations in the small intestines of lambs at slaughter (P = 0.004) but that there was no statistical relationship with environmental parameters such as minimum and maximum temperature, rainfall or hours of sunshine. In an analysis along the length of the intestinal tract of lambs, campylobacters were isolated from 80% of small intestine and 30% of rumen samples, but not from the true stomach, large intestine or caecal samples. A survey of sheep grazing on salt marsh, fell (upland) and farm pasture showed that the isolation rate of thermophilic campylobacters in fresh faeces was substantially lower (29.3% (n = 420)) than that from the small intestine of lambs at slaughter. No significant difference (> 0.05) was found between the isolation rate of campylobacters from faeces sampled in late spring/early summer and autumn, nor between the different types of grazing.

Abattoirs↗

The colonization of turkeys by thermophilic campylobacters.

The rate at which five broods of turkey chicks became colonized by thermophilic campylobacters was investigated. Day-old chicks were normally free of campylobacters on arrival on the farm with colonization beginning within 7 d. The carriage rate was 100% by day 14 in three of the broods and by day 21 in the other two. Higher carriage rates were obtained with enrichment procedures than with direct plating. Two broods were investigated over an extended interval for the number of campylobacters shed in their faeces. In brood A, Campylobacters increased from day l to day 14 concomitant with increase in the carriage rate and in the number of chicks with diarrhoea. By day 39, when the birds were sold to other farms, the excretion rate had reached 6 x 10(7) campylobacters g-1 fresh faeces. Brood B. was monitored over 91 d and showed peaks in Campylobacter numbers on days 19 and 75, corresponding to peaks in the number of diarrhoeic samples. The introduction of new birds into the brood resulted in an increase in the Campylobacter population and in the number of birds with diarrhoea. Campylobacter jejuni was the only species isolated but comprised several different biotypes. Analysis of the number of campylobacters at different sites along the gastrointestinal tract of mature turkeys at slaughter showed that numbers increased with distance from the beak and were highest in the caeca.

Animals↗

The seasonal variation of thermophilic campylobacters in beef cattle, dairy cattle and calves.

The epidemiology of clinical cases of campylobacter in temperate climates shows a striking seasonality. In the search for a seasonal environmental reservoir changes in the carriage rate and population size of campylobacters in bovine hosts with time have been measured. Most probable number (MPN) methodology was used to enumerate thermophilic campylobacters in samples taken from the small intestines of beef cattle at slaughter and the fresh faeces of four dairy herds and new-born calves. Statistical analyses revealed significant evidence for seasonal periodicity in the data from dairy herds (P = 0.044). Not only was there a departure from constancy within a 12-month interval but these data revealed a true seasonality, that is, the same periodicity in numbers from one year to the next. Each herd had two peaks per year, in approximately spring and autumn. Peaks coincided in herds on neighbouring farms but those on farms in the north preceded those on farms in the south by 2 and 1 months, respectively (P = 0.0057). Intestinal carriage by beef cattle at slaughter was 89.4% (n = 360) with an average MPN campylobacters per gram fresh weight (MPN gfw-1) of 6.1 x 10(2). Average MPN gfw-1 in faeces from the dairy herds and calves were 69.9 (S.D. 3) and 3.3 x 10(4) (S.D. 1.7 x 10(2)). There was no evidence of seasonal periodicity in the size of the campylobacter population in beef cattle at slaughter. Calves were campylobacter free at birth but became colonized with a few days.

Animals↗

Sound localization: information theory analysis.

Sound localization has been studied extensively. Curiously, although much is known about factors that affect errors, little is known about factors that influence response time. Three experiments were performed in an attempt to identify the separate influence of each of several different factors. All trials used a single broadband noise signal emanating from one of a subset of six loudspeakers equally spaced around the participant in the azimuthal plane. Both the size of the subset and the balance of relative probabilities from speaker to speaker were altered to evaluate the relationship between information content and the dependent variable, choice reaction time. Choice reaction time was found to be related to the information content of the sound stimulus in all cases. It was also found to be related to the presence of pairs of speakers that were symmetrically opposed in front of the behind the participant. Models of choice reaction time in a sound localization task have clear implications for practice. For example, they suggest that multiple auditory collision warnings may endanger drivers.

Adult↗

Isolation of vero cytotoxin-producing Escherichia coli O157 from wild birds.

In a survey of wild birds (mainly gulls), 0.9% of the bacterial isolates from faecal samples at an urban landfill site and 2.9% of bacterial isolates from faecal samples on intertidal sediments in Morecambe Bay were Vero cytotoxin-producing Escherichia coli O157. Isolation procedures employing commonly used cultural methods were hindered by the selection of a large number of false positives. The only procedure which resulted in the isolation of E. coli O157 from bird faecal samples was: enrichment (18 h) in a selective tryptone soya broth followed by filtration using hydrophobic grid membranes and growth on Chromagar O157. The majority of isolates selected as potential E. coli O157 by characteristic growth on Chromagar O157 could be eliminated by subsequent growth on CT-SMAC or CR-SMAC. This second identification (characterization) stage reduced the number of potential E. coli O157 requiring further confirmation by typing methods (serotype and Vero cytotoxin) by more than 70%.

Animals↗

Seasonality of thermophilic Campylobacter populations in chickens.

The small intestines and caeca of chickens were monitored for seasonal variation of thermophilic campylobacters over a 12-month period. There was a significant seasonal fluctuation in the carriage rate which correlated with the following environmental parameters: sunshine hours (P = 0.0003) and minimum (P = 0.007) and maximum temperatures (P = 0.003). The number of campylobacters in the small intestine and caeca also showed significant seasonal variation (P = 0.0008); however, the periodicity in the caeca was significantly different from that in the small intestine (P = 0.007). The numbers of Campylobacter in the caeca were significantly higher than those in the small intestine (P = 0.001). No significant correlation was found between the numbers of campylobacters in the caeca and small intestines and the environmental parameters monitored.

Animals↗

The use of selective and differential agars in the isolation of Escherichia coli O157 from dairy herds.

The use of selective and differential agars for the isolation of Escherichia coli O157 from the faeces of dairy herds was investigated. Out of the 614 isolates which were positive for one or more of the selective criteria employed in the media only four proved to be E. coli O157. Ninety-nine per cent of the isolates were false positives. The procedure which resulted in the isolation of E. coli O157 from faecal samples was enrichment in modified Tryptone Soya Broth supplemented with novobiocin and subsequent growth on Chromagar O157.

Agar↗

The ras oncogene and tumour metastasis: observations on murine cells transfected with activated human c-Ha-ras.

Transfection of cells with cloned genes or total genomic DNA offers a means for studying aspects of neoplastic behaviour. We have used this method to examine whether incorporation of the cloned 6.6-kilobase (kb) fragment of DNA containing the mutant c-Ha-ras human oncogene can confer metastatic capability on murine NIH 3T3 cells. Cells co-transfected with the mutated ras gene and the neomycin resistance marker pSV2neo were selected by culture in neomycin. On subcutaneous inoculation into MF 1 nude mice, these cells proved to be tumourigenic with short latent periods (approximately 14 days)--nude mice were used to circumvent immunological rejection of the mouse cells expressing the product of the human oncogene. Transfectants were capable of lung colonisation after intravenous injection, but there was no evidence of spontaneous metastasis at autopsy, or on histological examination of the lungs and other organs, 90 days after inoculation. Incorporation of the transfected oncogene was confirmed by Southern blotting and its expression by dot-blot hybridisation and immunoprecipitation. The results in this experimental system indicate that transfection of a mutated human ras oncogene into non-neoplastic 3T3 cells can confer part of the metastatic phenotype, namely lung colonisation, but is not by itself sufficient to induce spontaneous metastatic behaviour.

Animals↗

Tumorigenic transformation of mouse NIH 3T3 cells by transfection with plasmid pSV2neo.

This study reports high incidence tumourigenic activity of the pSV2neo plasmid demonstrated by transfection of NIH 3T3 cells. The plasmid is frequently used as a dominant selectable marker for confirming successful gene transfection, and the findings indicate a need for caution in interpretation and design of assays for oncogenes which use co-transfection and subsequent selection with neomycin.

Animals↗

Monoclonal antibodies specific for stalk differentiation in Dictyostelium discoideum.

We have produced two monoclonal antibodies specific to the stalk cells of Dictyostelium discoideum fruiting bodies. Both monoclonal antibodies react with high molecular weight proteins previously found to be stalk-specific by two-dimensional gel analysis. One antibody (JAb 1) is specific for a single protein of apparent molecular weight 310 000 which first appears when overt stalk differentiation begins at 20 h. The other monoclonal antibody (JAb 2) is also stalk-specific, though earlier in development it binds to proteins extracted from both prestalk and prespore cells of the migrating slug. It reacts with two proteins in stalks, one of apparent molecular weight 430 000 which is first detected during tip formation at 12 h and a lower molecular weight protein (310 000) detected from 20 h. Although several markers are available for the investigation of prespore/spore differentiation there is a distinct lack of suitable prestalk/stalk markers. The monoclonal antibodies described here are highly specific stalk markers and should prove useful in the study of cell proportioning and terminal differentiation.

Antibodies, Monoclonal↗

Genetic analysis by mitotic recombination in Dictyostelium discoideum of growth and developmental loci on linkage group VII.

A genetic study was made of two developmental loci, stmA (mutations of which give 'streamer' mutants with large aggregation territories) and fgdB (mutations of which give a class of 'aggregateless' mutant unable to respond to exogenous cyclic AMP signals), and three growth-related loci, couA, tsgK and bsgB (sensitivity to growth with coumarin, temperature sensitivity for growth and inability to grow on Bacillus subtilis, respectively). These loci, which have previously been located on linkage group VII, were found by mitotic recombination studies to lie proximal to the recombinant selector cobAI (resistance to cobaltous chloride) with the order: centromere-couA-tsgK-(stmA-bsgB)-fgdB-cobA. Analysis of haploid segregants derived from 374 recombinant diploids allowed relative map distances to be calculated and revealed that tsgK, stmA, bsgB and fgdB lay as a loosely clustered group close to cobA. Two independently isolated mutations at another streamer locus, stmF, were found to be associated with a diploid instability phenomenon such that heterozygous (stmF/+) diploids which became homozygous at the cobA locus on linkage group VII by mitotic recombination reverted very rapidly to the haploid state. Reversion studies with the tsgK21 marker revealed that partial revertants (or suppressors) at this locus frequently introduced secondary developmental or growth-related mutations by an unexplained mechanism.

Chromosome Mapping↗

Discharge residue from mercury fulminate-primed ammunition.

The scarcity of mercury in discharge residue particles from mercury-containing ammunition was investigated. It was found that 86% of the mercury was vaporized after discharge, mainly via the muzzle, 88% of which was not detectable by SEM. Of the 0.16% exiting via the breech, only approximately 40% was detectable by SEM. Only a small proportion of this was deposited on the firer. The recovered mercury did not make a significant contribution to the elemental composition of the discharge residue particles. The rate of loss of mercury from the spent cartridge case was also examined but was found to be of no practical value in estimating time of discharge.

Firearms↗