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Biomedical subjects

J Sabliński

Publications and source records attributed to J Sabliński.

14 recordsLinked to original sources
PubMed1994

Secretory response of the vessel wall to DDAVP and venous occlusion in von Willebrand's disease.

The aim of this study was to compare the secretory response of the vascular wall in vivo to DDAVP (i.v. 0.3 microgram/kg, 30 min) and to venous occlusion (VO, 20 min) in control healthy subjects, patients with von Willebrand's disease type I (vWd I) and patients with von Willebrand's disease type III (vWd III). In controls (n = 10) and vWd I (n = 12), DDAVP induced a 2 to 3-fold rise in plasma von Willebrand factor antigen (vWf: Ag), factor VIII coagulant activity (VIII: C) and tissue--type plasminogen activator antigen (t-PA:Ag). VO was less effective in increasing vWf: Ag and VIII:C but produced a greater rise in t-PA:Ag. Large increments (over 10-fold) were observed in plasmin-alpha 2-antiplasmin complexes following both stimuli. In vWd III (n = 10), DDAVP and VO failed to increase vWf:Ag, VIII:C and t-PA:Ag. No significant changes in plasmin-alpha 2-antiplasmin complexes were observed in this group. Moreover, the baseline t-PA:Ag values were significantly lower in vWd III (2.17 +/- 1.13 ng/ml) than in controls (4.84 +/- 1.97 ng/ml, p < 0.001). A significant increase in urokinase--type plasminogen activator antigen (u-PA:Ag) was found only in controls after VO. Neither controls nor patients with vWd showed any changes in plasma fibronectin levels following DDAVP. The low t-PA:Ag results and the abnormal fibrinolytic response to DDAVP and VO in patients with severe (type III) vWd indicate that their endothelial cell abnormality is more extensive than the defect in the synthesis or release of vWf.

Adult↗
PubMed1994

[Comparison of platelet concentrates obtained using blood cell separators with continuous flow: CS-3000 and Cobe-Spectra].

During a platelet apheresis procedure on CS-3000 the platelet concentrate (Pc) is centrifugated for about 1.5 h. In the Cobe system Pc is gradually collected in a container located outside the machine. Influence of both techniques on platelet viability and function was examined in vitro. MPV, hypotonic stress response (HSR), aggregation using ADP, activity and percent leakage of lactic dehydrogenase (LDH) were similar in both groups. ATP contents in platelets (Plts) obtained on CS-3000 reached 5.18 +/- 0.75 mumol/10(11) Plts and was significantly lower than in Plts collected on Cobe-Spectra: 7.26 +/- 1.00 mumol/10(11) Plts. The functions of Plts obtained on both blood cell separators, immediately after collection, appeared to be correct. ATP level reduction in Plts from CS-3000 suggests that prolonged centrifugation leads to Plts activation which can affect cells during storage.

Blood Platelets↗
PubMed1994

[Evaluation of platelet quality obtained using conventional methods or from buffy-coat and stored at room temperature].

A comparison has been made between platelet concentrates (Pcs) obtained from buffy coat (Bc) and from platelet rich plasma. The method of preparation proves to have an platelet properties in vitro. Both types of Pcs were investigated immediately after isolation as well as during the 5 day period of storage. It has been confirmed that due to the new technique the number of white cells in Pcs could be reduced 25 fold (to 2.79 +/- 1.68 x 10(6)/unit). It has also been showed that traditional way of obtaining Pcs provokes a greater platelet activation than the new technique. The storage conditions of Pcs obtained from Bc must however be practically defined because when CLX containers are used, afer 5 days an excessive pH increase (to 7.59), aggregation decline and HSR weakening are observed.

Blood Component Removal↗
PubMed1992

AIDS in Poland.

Explore the source record for details and available documents.

Acquired Immunodeficiency Syndrome↗
PubMed1991

[Therapeutic plasmapheresis in patients with multiple myeloma].

The effectiveness of therapeutic plasmaphereses with antineoplastic chemotherapy was evaluated in 25 cases of plasmocytic myeloma in stage III of progression of the proliferative process. The indication to plasmapheresis was hypergelification of serum with clinical symptoms of central nervous system disturbances, renal failure in various stages of progression, intensification of coronary symptoms, bleeding tendency. Good effects with reduced level of total protein and monoclonal protein in serum by 30-80% with regression of clinical symptoms caused by serum hypergelification were obtained in 11 cases. In the remaining patients clinical improvement of varying degree was noted when the level of total and monoclonal protein in the serum fell by 10-29%.

Adult↗