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J Sasahara

Publications and source records attributed to J Sasahara.

At least 19 recordsLinked to original sources
PubMed1983

Replication of hog cholera virus in porcine alveolar macrophage cultures.

Eight hog cholera viral strains were tested for virulence for pigs and ability to replicate in porcine alveolar macrophage cultures. Of them, five were virulent and replicated well in the macrophage culture. The other three were avirulent and grew less remarkably in this culture than them. It was suggested that the ability of the hog cholera viral strains to replicate in porcine macrophages might be correlated with their virulence.

Animals↗
PubMed1980

Swine vesicular disease viruses isolated from healthy pigs in non-epizootic period. I. Isolation and identification.

A total of 524 fecal samples were collected from healthy swine of 36 hog farms scattered all over Japan from which had been detected neutralizing antibody against swine vesicular disease (SVD) virus. A virus was isolated from 21 of them. It was neutralized by antiserum against SVD virus. Of the 21 samples, 19 were derived from six farms, in areas which remained to be free from SVD in the past years and two from a farm where SVD broke out 18 months before. The cross-neutralization test was carried out with seven strains of SVD virus isolated from healthy pigs (SVDV-H) and five strains of SVD virus isolated from diseased pigs (SVDV-D). There was, however, no significant difference in antigenicity between the two groups. Some strains of SVDV-H were antigenically close to the Faulkner strain of Coxsackie B5 virus, and others to the freshly isolated strain of this virus. Neutralizing antibody of low titer against SVD virus was detected from pigs kept in areas free from SVD. It was presumed to have been produced in these pigs involved in silent infection with this virus.

Age Factors↗
PubMed1980

Swine vesicular disease viruses isolated from healthy pigs in non-epizootic period. II. Vesicular formation and virus multiplication in experimentally inoculated pigs.

An infection experiment was carried out on pigs with swine vesicular disease virus isolated from healthy pigs (SVDV-H). Inoculation was done by two routes, intradermal in the coronary band of the foot and oral. Observation was made on the formation of vesicles and their spread, the virus contents of serum, swab of the oral cavity, and feces, the vicissitude of neutralizing antibody titers, and the distribution of virus in the body. From its results the pathogenicity of virus was judged. In the pigs inoculated intradermally there was a difference in the extension of the area involved in vesicular formation between any two strains of virus. That is, vesicular formation was restricted to the site of inoculation, involved the site of inoculation and the sole of the hoof, or spread over the oral and nasal regions. In every pig, however, vesicles developed only for 2 approximately 5 days after inoculation. After that, repair progressed rapidly. Some strains caused viremia, which was mild. The virus was detected from the site of vesicular formation, but not from any organ. Neutralizing antibody began to be detected 3 days after inoculation. Its titer reached a plateau about 10 days later. In the pigs inoculated perorally, no vesicles were formed. The virus was only detected from the tonsils and the intestinal contents. These findings made it clear that SVDV-H was less pathogenic than swine vesicular disease virus isolated from diseased pigs.

Animals↗
PubMed1975

Outbreaks of swine vesicular disease in japan: virus isolation and epizootiological survey.

Outbreaks of a vesicular disease occurred among pigs in Kanagawa and Ibaraki Prefectures in Japan in November, 1973. Another outbreak was observed in Aichi Prefecture in December. The clinical signs of the disease observed included fever and vesicular lesions on the coronary bands, bulbs of the heel and in the interdigital spaces. In some pigs, vesicular lesions were observed on the snout, tongue and skin overlying the legs and abdomen. All the vesicular samples produced cytopathic changes on cultures of primary swine kidney cells of PK-15 cells. Three isolates of cytopathic agents tested were identified as swine vesicular disease virus from their physicochemical properties and antigenicity. The virus strains isolated from vesicular epithelial samples obtained from Ibaraki, Kanagawa and Aichi Prefectures were designated as Japan/Ibaraki/1/73, Japan/Kanagawa/1/73 and Japan/Aichi/1/73 strain, respectively. An outbreak of the disease among pigs due to swine vesicular disease virus was confirmed by the serum neutralization test with serum samples collected from pigs on affected farms. Approximately 80% of the pigs housed in affected shed showed high levels of neutralizing antibody titers. This is the first to report an occurrence of swine vesicular disease among pigs in Japan.

Animals↗