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Biomedical subjects

J Seidel

Publications and source records attributed to J Seidel.

At least 19 recordsLinked to original sources
PubMed1992

"You'll feel me touching you, sweetie": vaginal examinations during the second stage of labor.

Videotapes of women during the second stage of labor and their caregivers were examined in this descriptive study to determine how caregivers performed sterile vaginal examinations. Conversational analysis techniques were used to analyze the data. Results showed that the examinations were performed in a ritualistic manner by all caregivers, and the way the ritual was enacted repeatedly demonstrated the power of the caregivers over the women. The most common reason for performing the procedure, to help the woman push better, seems to be specific to the second stage of labor and is not described in the literature. The authors offer suggestions for better use of the examination, and recommend that it be performed far less frequently than is common.

Adolescent↗
PubMed1992

[Kinetic measurement of GPT with the microtiter plate].

Experiences with a kinetic method for measurement of SGPT in microtiter plate using an automatic sample processing device and Medusa software (Biotest) were evaluated. The correlation coefficient in parallel assessment of samples in a clinical routine laboratory was found to be 0.935 (p < 0.0001). The correlation coefficient comparing the internal standard dilution with actual recordings was found to be 0.999 (p < 0.0001). Automated screening of SGPT in microtiter plates seems thus to be reliable as well as feasible in blood bank routine.

Alanine Transaminase↗
PubMed1990

[Dendrite-free GABAergic neurons of the visual system in the rat].

Spinefreie GABAergic neurons in the visual cortex of the rat were studied with the Golgi-Kopsch-method and immunohistochemical methods against GABA. They show differences of their axonal arborizations, axonal surfaces and of their axonal terminal formations. We found large spinefree neurons, spinefree neurons with strictly axonal fields, spinefree neurons with dense axonal fields, spinefree neurons with axons forming arcades, spinefree neurons with columnar axons and spinefree neurons with clewded axonal terminals.

Animals↗
PubMed1989

Cloning and expression of human steroid-sulfatase. Membrane topology, glycosylation, and subcellular distribution in BHK-21 cells.

A 2.4-kilobase cDNA clone for human steroid-sulfatase (STS) was isolated and sequenced, which encoded an enzymatically active protein. The deduced amino acid sequence comprises 583 amino acids with an N-terminal signal peptide of 21 or 23 residues and four potential N-glycosylation sites. Two of the N-glycosylation sites are utilized and were localized to the asparagine residues 47 and 259. STS has the solubility properties of an integral membrane protein. The resistance of STS toward proteinase K after translocation into microsomes suggests that most, if not all, sequences of STS are exposed at the luminal side of microsomes. The deduced amino acid sequence predicts two membrane-spanning domains (amino acids 185-211 and 213-237) separated by a helix-breaking proline residue. We propose for STS a three-domain model. Two glycosylated luminally oriented domains of 161 and 346 residues are separated by a hydrophobic domain spanning the membrane twice in opposite directions. STS expressed in BHK-21 cells is located predominantly in the endoplasmic reticulum; smaller fractions are found in the Golgi, at the cell surface, multivesicular endosomes, as well as in lysosomes. The stability of STS in lysosomes may be related to the high homology of the two luminal domains of STS with the lysosomal sulfatases, arylsulfatase A, and arylsulfatase B. In spite of its similarity with these two lysosomal sulfatases, STS does not contain mannose 6-phosphate residues and is transported to lysosomes by a mannose 6-phosphate receptor-independent mechanism.

Amino Acid Sequence↗
PubMed1989

Differences in response of acetylcholinesterase to diisopropylfluorophosphate in the mesencephalic raphe region. A microelectrophoretic, histochemical, and biochemical study in albino rats.

To demonstrate the DFP-effects on the AChE of the mesencephalic raphe region albino rats were investigated using the pharmaco-histochemical method of Butcher et al. (1975). At several times after 1 mg DFP/kg body weight, the histochemical reactions in certain AChE-containing neurons were measured semiquantitatively and compared with biochemical data. Additionally, the multiple forms of water-soluble AChE were separated by microelectrophoresis. Only the electrophoretical results indicate changes of the structural AChE composition 5 d after DFP administration.

Acetylcholinesterase↗
PubMed1988

Recombinant human granulocyte-macrophage colony stimulating factor (rh GM-CSF) after bone marrow transplantation.

Bone marrow transplantation improves the chances of survival in a variety of hematological malignancies. However, infectious complications during the post-transplant phase contribute significantly to morbidity and mortality. To reduce the duration of granulocytopenia, which is approximately 20 days after BMT, in this study patients with ALL, relapsed or high-grade NHL, relapsed or refractory HD, or Neuroblastoma stage III/IV, were given rh GM-CSF to assess the effects on hematological and immunological reconstitution after conditioning therapy and BMT. The results of 9 patients are presented. After autologous BMT and subsequent rh GM-CSF therapy, a peripheral blood neutrophil count of 500/microliters was reached within 8-12 days, i.e., between 7 and 10 days earlier than would have been expected without rh GM-CSF. Furthermore, it appeared that rh GM-CSF was useful in case of insufficient bone marrow regeneration post autologous transplant. The influence of rh GM-CSF after allogeneic BMT is not yet clear. Further studies will be necessary to evaluate the potential of this promising new drug after BMT.

Adult↗
PubMed1987

Influence of beta adrenergic blockers and their combinations with anticholinergic drugs on epileptiform activity in rabbit hippocampus.

The influence of alpha- and beta-receptor antagonists combined with drugs which act as antagonists at muscarinic and nicotinic receptor sites on epileptic focus produced by topic penicillin application was investigated. The beta-receptor blocking agent caused a faster and more intense activity of epileptogenic focus in comparison with activity in control animals, but the number of seizures decreased relatively for 25%. N-receptor antagonists diminished the facilitating effect of the beta-receptor antagonist on focal activity. Combination of m- and beta-receptor antagonists did not influence the spike activity of the focus. Treatment with alpha-receptor antagonists enhanced spikes and number of seizures only insignificantly. The combination of alpha- and n-receptor blocking substances does not influence the development of the epileptic process. The alpha-receptor antagonist together with the m-receptor blocking drug induces a facilitation of spike activity.

Administration, Topical↗
PubMed1986

The heterogeneity of the nucleus raphes dorsalis in albino rats. A fluorescence histochemical and microelectrophoretic study.

In albino rats, the nucleus raphes dorsalis (NRD) of the midbrain was investigated using a sensitive fluorochroming procedure to detect indolamines in combination with a microelectrophoretic method to demonstrate the isoenzyme pattern of acetylcholinesterase (AChE). Based on the fluorescence histochemical appearance, 3 types of indolaminergic cells were identified in the various areas of the NRD. According to data of the literature and in agreement with our own results, the morphological heterogeneity of the NRD was shown. Significant differences of AChE specific zymograms of NRD regions (subgroups) demonstrate that organization of NRD is heterogeneously proved not only by morphological results but also in respect of enzyme constitution.

Acetylcholinesterase↗
PubMed1986

Amebic infections in asymptomatic homosexual men, lack of evidence of invasive disease.

A survey for enteric infections in 140 asymptomatic homosexual men who attended a community clinic revealed a high prevalence of infection with Entamoeba histolytica (27.1 per cent) and Giardia lamblia (15.7 per cent). In contrast, the prevalence of elevated indirect hemagglutination (IHA) titers (greater than or equal to 1:128), which indicate invasive amebiasis, was low (5.7 per cent). Our findings suggest that only a limited amount of invasive amebic disease is occurring in this group of homosexual men.

Adolescent↗