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Biomedical subjects

J Seifert

Publications and source records attributed to J Seifert.

At least 19 recordsLinked to original sources
PubMed1979

[Extension of graft survival time by means of a modification of the treatment with autologous blood].

A modification of the therapy with patients' own blood was achieved by the application of blood components together with an adjuvant. In a defined experimental rat model it was to be clarified whether it is possible to sensitize animals with an antibody directed against skin together with an adjuvant, in order to produce an anti-antibody, which is able to protect a consecutive skin graft from rejection. In a similar way animals were sensitized with cells from rats previously immunized against skin from the donor. By this treatment it is possible to improve survival time of skin grafts. Whereas the prolongation of survival time after pretreatment with skin antibodies can be explained by an anti-antibody, which was found in the serum of the animals, such an anti-antibody could not be detected in the serum of animals, pretreated with "sensitized" cells. Possibly the treatment with cells induces an unspecific effect, which can be achieved also with normal cells. It is conceivable that this principle of the prolongation of skin graft survival time by immunization with certain components of the blood is not only useful in transplantation immunology, but also in other diseases, i.e. autoimmune diseases or allergic reactions.

Animals↗
PubMed1979

Alterations in metabolism of cytidine components in rat liver after oral administration of butylated hydroxytoluene (in vivo study).

The administration of the antioxidant, butylated hydroxytoluene (BHT) to rats decreased the utilization of [2-14C]orotic acid for the synthesis of cytidine nucleotides in the acid-soluble extract and RNA of the liver. The specific activity of the uridine components was slightly decreased. The depression of the specific activity of the cytidine components depended on the dose of the drug. Simultaneously preformed [U-14C]cytidine in experimental rats was to a higher degree transported to the liver and incorporated into RNA cytosine; its deamination was markedly suppressed. Both phenomena depend on the BHT dose. The concentration of both the uridine and the cytidine components of the acid-soluble extract remained unaffected by the administration of BHT. The utilization of [2-14C]orotic acid for the synthesis of DNA cytosine was depressed after the administration of BHT; by contrast, the specific activity of DNA thymine was higher. The incorporation of [1-14C]palmitic acid into microsomal phospholipids was not substantially influenced over the dose range 25--500 mg BHT/kg. The specific activity of neutral lipids in microsomes increased.

Animals↗
PubMed1979

Microcirculation and blood volume in rats before and after spinal cord injury.

Blood flow, cardiac output, blood pressure, indicator mixing time and blood volume were measured in rats before and after spinal cord injury. After cutting off the spinal cord blood flow decreases markedly in the paraplegic area but also in the main organs of the animals. Only the blood supply of the lung increases by this manipulation. As blood volume and the blood pressure is decreased and the indicator mixing time delayed the situation of the spinal cord injury can be compared with the situation after acute blood loss. Therefore it is proposed to include circulatory disturbances into the conception of the acute spinal shock situation.

Animals↗
PubMed1979

[Scintigraphic and quantitative measurement of the gastric wall blood-flow of the dog (author's transl)].

In the examinations presented the gastric wall blood-flow of the dog was measured scintigraphically by using the gamma camera and quantitatively by making use of the microsphere method under different functional conditions. In doing so it became apparent that not only the individual functional stomach regions (corpus-antrum) but as well the individual stomach wall layers (mucosa-muscularis) can show a counter blood flow procedure. The two methods mentioned above have proved to be suitable both for the determination of the gastric wall blood flow in the individual layers and the optical reproduction of the regional blood-flow procedure.

Animals↗
PubMed1979

[Absorption of a proteolytic enzyme originating from plants out of the gastro-intestinal tract into blood and lymph of rats (author's transl)].

The absorption of the proteolytic enzyme bromelain was investigated in adult rats. Bromelain was labelled radioactively with 125-Iodine and intraduodenally applied. During the observation time of 6 hours blood- and lymphsamples were collected and their radioactivity measured. By radiochromatography high molecular protein could be separated from low molecular protein fragments. By means of a rabbit-anti-bromelainserum bromelain could be identified in the agar-double diffusion technique. Results show that adult rats can absorb bromelain up to 40% in a high molecular form. This observation explains the increased proteolytic activity in serum after oral application as well as the clinical effect concerning the treatment of edema or hematoma.

Animals↗
PubMed1979

[Absorption and distribution of cytoplasmic organ lysates (conjunctisan A eye drops) following intraconjunctival application (author's transl)].

The absorption and transport pathways of Conjunctisan A were determined, and its mode of action clarified, by means of radioactive labeling. A dose of 0.5 ml of this drug, labeled with I131, was applied to the conjunctival sacs of rats and rabbits. After six hours there was highly significant enrichment in the ocular tissues. The highest degree of enrichment was found in the uvea, i.e., the ciliary body, choroid and retina. However, there was also moderate enrichment in the lens and the vitreous. It was proved immunologically that the radioactivity was identical with the original Conjunctisan A molecule. In particular, it as demonstrated that the enriched Conjunctisan A in the aqueous humor precipitates with the specific antibody.

Absorption↗
PubMed1978

[Antigen-elimination technic. An in vivo method for studies of the human immune response against horse gamma globulin].

In 20 patients treated with antilymphocyte horse gamma-globulin (ALG) the immune response was tested by means of the antigen elimination technique before, during and after ALG-therapy. The comparison of this method with agargel diffusion test, indirect hemagglutination and skin tests showed that immunization of patients against horse protein is indicated earlier and more reliable with the antigen elimination. Therefore this technique should be utilized during a therapy with foreign proteins especially when the results of other immunological test methods are insufficient.

Animals↗
PubMed1978

Increased transport of (G-14C) cytidine into rat liver after administration of alpha-hexachlorocyclohexane.

The transport of cytidine into liver over a wide dose range (0.003--100mumol per animal) proceeds as an nonsaturabel process. After the administration of alpha-hexachlorocyclohexane (alpha-HCH)1 the transport of (G-14C) cytidine is markedly activated if the concentration of nucleoside administered is low; the differences between the control and experimental group disappear after the administration of higher doses of the nucleoside. The incorporation of (G-14C) cytidine into RNA cytosine is enhanced after the administration of alpha-HCH. The degree of utilization of labeled cytidine for the RNA synthesis decreases in proportion to the logarithm of its dose, both in the control and in the experimental group. Increased transport of (G-14C)cytidine is observable even between the 4th and 6th day after the administration of a single dose of alpha-HCH.

Animals↗
PubMed1978

Persistence of fungicide Euparen on strawberry and/or in some canned products of strawberry.

There was studied the persistence of Euparen(dichlofluanid) on strawberry and in some canned products of strawberry, respectively. The rate of decrease of dichlofluanid residues on the leaves of strawberry is proceeding according to the first-order reaction kinetics in the initial 21 days. The content of dichlofluanid residues on strawberry treated twice with fungicide varies from 0.5 to 1.0 ppm, for strawberry sprayed three times from 1.0 to 2.0 ppm, respectively. The significant decay of dichlofluanid residues occurs during the technological procedures applied. The sensoric analysis of strawberry has proved the negative influence of the third spray of fungicide in the initial days. No significance differences between strawberry treated with fungicide and control has been found in flavor and taste of the canned products investigated.

Aniline Compounds↗
PubMed1978

Liver growth, biosynthesis of cytidine nucleotides and level of cytochrome P-450 in rat liver after administration of alpha-hexachlorocyclohexane.

The biosynthesis of cytidine nucleotides and the level of microsomal cytochrome P-450 in intact and regenerating rat liver after repeated administration of alpha-hexachlorocyclohexane (alpha-HCH) were compared. In alpha-HCH treated animals the utilization of [2-14C] orotic acid for the synthesis of cytidine nucleotides is suppressed. In 24-h regenerating liver the incorporation of labelled orotic acid into cytidine nucleotides is markedly activated; the degree of activation is lower in regenerating livers of alpha-HCH treated animals. The changes in the level of cytochrome P-450 vary inversely with the changes in the utilization of [2-14C] orotic acid for the synthesis of cytidine nucleotides. The activity of cytidine triphosphate synthetase of liver cytosol increases shortly after the administration of alpha-HCH; uridine-cytidine kinase is enhanced in the later stages of the drug action. Within 15-45 min after the administration of alpha-HCH the uptake of [U-14 C] cytidine into the liver and its incorporation into RNA cytosine are increased. After the administration of the drug the uptake of [2-14 C] uridine and its incorporation into RNA uracil is also enhanced whereas its utilization for the synthesis of cytidine nucleotides of the acid-soluble extract as well as for the RNA cytosine are suppressed.

Animals↗