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Biomedical subjects

J Shay

Publications and source records attributed to J Shay.

14 recordsLinked to original sources
PubMed1992

Estrogen and progesterone withdrawal increases cerebral vasoreactivity to serotonin in rabbit basilar artery.

An in vitro animal model which examines the effects of sex hormone variations during the menstrual cycle on basilar artery reactivity is presented. Three groups of rabbits were utilized: a chronically depleted control group which received no further hormonal treatment after bilateral surgical oophorectomy (O), simulating menopause, and two groups of intact females, one of which was treated to mimic the estrogen and progesterone surge during the luteal phase (H) and the third group which was acutely estrogen and progesterone depleted after the luteal surges to simulate the immediate premenstrual state (W). We show that both acute and chronic estrogen and progesterone withdrawal significantly increase serotonin sensitivity (ED50) in basilar artery rings. There was no difference between groups for maximum contraction (Tmax) to serotonin, nor optimal resting tension. Furthermore, there was no difference in vasoreactivity and contractility to norepinephrine between groups. In order to distinguish between the effects of chronic and acute treatment we examined acute estrogen and progesterone superfusion in basilar artery rings from intact non-treated female rabbits. Acute superfusion of pre-contracted and non-pre-contracted artery segments resulted in significant dilatation only when supraphysiologic concentrations of estrogen and progesterone were used. We conclude that both acute and chronic female sex hormone withdrawal selectively increases cerebral vasoreactivity to serotonin.

Animals↗
PubMed1991

The effect of maternal supplementation with linoleic and gamma-linolenic acids on the fat composition and content of human milk: a placebo-controlled trial.

Total fat content and therefore total energy content and the content of essential fatty acids (EFAs) in milk are known to decline with prolonged breast feeding. In a placebo-controlled study a variety of evening primrose oil (Efamol) rich in linoleic and gamma-linolenic acids, or a matching placebo were given to 39 women for a period of 8 months starting between the 2nd and 6th months of lactation. Total fat and EFA contents of the milk declined in the placebo group but rose in the primrose oil supplemented group. A surprisingly high proportion of the supplemented dietary fatty acids could be accounted for by appearance in the milk. The milk composition can be readily manipulated by changing the fatty acid composition of the maternal diet.

Dietary Fats, Unsaturated↗
PubMed1985

Solid base working cast.

A technique is presented to facilitate the fabrication of single or multiple restoration die working casts. The technique allows a full remount potential for use especially in multiple centric relation record verification techniques. A die removal technique that will not damage the brass dowel pin is suggested.

Dental Casting Technique↗
PubMed1976

Cell volume and permeability of oxygen-and glucose-deprived retina in vitro.

Rabbit retina was deprived of O(2) and glucose in vitro for up to four hours at 37 C. Intracellular volume was measured, using inulin as an extracellular marker. After a 30-minute latency, cells swelled rapidly to more than twice normal volume while extracellular volume was unchanged. Intracellular accumulation of water was not reversed by resupply of oxygen and glucose. Permeability to small molecules was assessed with mannitol. The ratio of mannitol space to inulin space averaged 1.0 in controls. This ratio remained 1.0 up to 30 minutes of deprivation, but increased to 1.2 by 60 minutes. Permeability to large molecules was assessed from the rate of loss of isotopically labeled cell protein into the medium. There was no difference between control and deprived retinas up to three hours.

Animals↗
PubMed1976

Retina subjected to components of ischemia in vitro. Selective vulnerability and minimum lethal exposure of neurons and glia to oxygen and/or glucose deprivation and to loss of exchange with incubating medium.

Rabbit retinas were incubated at 37 C in media lacking oxygen, glucose, or both, or sealed in a small compartment without medium to convert them to a "closed system." They were then returned to control medium before being fixed for microscopy. Other retinas were incubated only in control medium and then fixed. Conversion of the retina to a closed system caused irreversible damage to all cell types within 40 minutes. Combined deprivation of oxygen and glucose also irreversibly damaged the neuronal cells within 40 minutes, but Mueller cells,the principal glial cells of the retina, were not irreversibly altered by 90 minutes of the deprivation. Deprivation of oxygen alone caused irreversible damage to receptor cells in 80 minutes, but the cells of the inner nuclear layer, ganglion cells, and Mueller cells retained normal structure for at least 180 minutes. Deprivation of glucose alone damaged receptor cells in 160 minutes and the other neuronal cells in 180 minutes, but did not irreversibly damage Mueller cells by 200 minutes.

Animals↗
PubMed1975

Economy of effort in electron microscope morphometry.

Statistical techniques known as the analysis of variance make it possible for the electron microscopist to plan work in such a way as to get quantitative data with the greatest possible economy of effort. This paper explains how to decide how many measurements to make per micrograph, how many micrographs per tissue block, how many blocks per experimentally treated organ, and how many organs per experimental treatment group.

Analysis of Variance↗
PubMed1974

Reconstruction of mammalian cells from nuclear and cytoplasmic components separated by treatment with cytochalasin B.

Mouse L929 cells were separated into enucleated cytoplasmic components (cytoplasts) and nucleated subcellular fractions (karyoplasts) in the presence of cytochalasin B. Karyoplasts from cells containing tritiated nuclei were fused, using inactivated Sendai virus, to cytoplasts from cells containing large (1.0-mum diameter) latex spheres in the cytoplasm. Mononucleated cells containing radioactive nuclei and large latex spheres in the cytoplasm were observed among the products of the fusion reaction. Some of these cells were in mitotic configurations. The results indicate that cells capable of undergoing mitosis can be reconstructed from the products of cellular enucleation in the presence of cytochalasin B.

Animals↗
PubMed1973

Electron microscopy of cat spinal cord subject to circulatory arrest and deep local hypothermia (15 C).

Circulatory arrest to the lumbar spinal cord of adult cat was produced by occlusion of the descending aorta and concurrent arterial hypotension. Local hypothermia of the cord was induced by paraffin oil at 5 C, which was circulated over the exposed surface of the cord, using the laminectomy wound as a trough. Intramedullary temperature was 15 C at a depth of 5 mm. In 10 control animals oil at 37 or 5 C was circulated over the exposed cords (normal-normothermic and normal-hypothermic controls with 1 and 2 hours hypothermia). Three animals had circulatory arrest and recirculation in normothermia (ischemic-normothermic) and 3 in hypothermia (ischemic-simultaneous hypothermia). Three had circulatory arrest and 15 minutes of recirculation in normothermia followed by 1 hour of hypothermia (ischemic-delayed hypothermia). The medial and lateral portions of the anterior gray horns of the last lumbar spinal segment were studied in the light and electron microscopes. Ischemic-normothermic tissue showed 20% shrinkage in mean areas of neuronal perikarya and massive "watery" swelling of astrocytic cell bodies and processes. Within neuronal perikarya and dendrites, cytoplasm increased in electron density, ribosomes dispersed, Golgi apparatus swelled and mitochondria swelled with loss of matrix density and disruption of cristae. Axons and axon terminals did not increase in size, but mitochondria within these structures doubled in size without loss of matrix density or change in pattern of cristae. Synaptic vesicles were no longer uniform in size, and they were clumped away from the synaptic cleft and diminished in number. Lysosomes were unchanged in appearance and size. Mitochondria of astrocytes underwent approximately fourfold enlargement without loss of matrix density or pattern of cristae. Bundles of astrocytic microfilaments were fragmented, spread apart and diminished in quantity. Oligodendroglia and endothelial cells were unchanged. Normal-hypothermic animals were similar to normal-normothermic except for clefts in rough endoplasmic reticulum of neurons and dendrites. These clefts were formed by a separation of the cisternal membrane from the adjacent row of ribosomal rosettes. Ischemic-simultaneous hypothermia animals had findings identical to normal-hypothermic animals. Ischemic-delayed hypothermia animals were similar to ischemic-normothermic animals except for less swelling of astrocytic processes, greater swelling of astrocytic mitochondria and less alteration of microfilaments. The findings show that ischemia in normothermia brings about alterations in virtually every organelle of the neurronal perikaryon except the lysosome. Simultaneous hypothermia in ischemia prevents the protean alterations of ischemia, whereas hypothermia delayed until after the ischemic episode only slightly modifies the cellular lesions found in ischemic-normothermic animals.

Animals↗
PubMed1973

Morphometry of an ischemic lesion of cat spinal cord.

Profiles in random electron micrographs of anterior gray matter of normal and ischemic cat spinal cord were measured with a planimetric computer. Analysis of 5600 area measurements revealed the following differences. Mitochondria of neuron cell bodies, axons, axon terminals and astrocytic processes were two to three times larger after ischemia. However, only 15% of mitochondria of axons and axon terminals and 5% of astrocytic processes lost their matrix density and pattern of cristae, compared to 49% of mitochondria of neuron cell bodies. Ischemia caused no significant changes in mean sizes of axons or axon terminals. Lysosomes in neurons were unchanged. The mean size of astrocytic processes increased more than threefold.

Animals↗
PubMedDate not supplied

Effects of linoleic and gamma-linolenic acids (efamol evening primrose oil) on fatty acid-binding proteins of rat liver.

We have studied the effects of Efamol evening primrose oil (EPO) on fatty acid-binding proteins (L-FABP) of rat liver. EPO contains 72% cis-linoleic acid and 9% cis-gamma linolenic acid. EPO has been clinically used for treatment of a number of diseases in humans and animals. EPO is also known to lower cholesterol level in humans and animals. Feeding of an EPO supplemented diet to rats (n = 9) for 2 months decreases the oleate binding capacity of purified L-FABP of rat liver whereas the palmitate binding activity was increased by 38%. However, EPO feeding did not alter the L-FABP concentrations significantly as measured by using the fluorescence fatty acid probe, dansylamino undecanoic acid. Endogenous fatty acid analysis of L-FABPs revealed significant qualitative and quantitative changes in fatty acid pattern after EPO feeding. EPO feeding decreased the endogenous palmitate level by 53% and oleate level by 64% in L-FABPs and also EPO feeding decreased the total endogenous fatty acid content from 62 nanomole per mg of protein to 42 nanomole per mg of L-FABP (n = 3).

Animals↗