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Biomedical subjects

J Shelly

Publications and source records attributed to J Shelly.

5 recordsLinked to original sources

The apolipoprotein CI A allele as a risk factor for Alzheimer's disease.

The E4 allele for the apolipoprotein E gene has been shown to be a significant risk factor for Alzheimer's disease. The gene is located in a conserved gene cluster on chromosome 19q12-13.2. Downstream from APOE is the gene for apolipoprotein CI. We had previously shown that the presence of a restriction site in the 5'end of APOCI (the A allele) was present at increased frequency in Alzheimer's patients and could also be considered as a risk factor for the disease. We have extended these studies and find that both familial and sporadic cases of Alzheimer's disease have a higher frequency of the APOCI A allele than control spouses. In addition, male patients with the APOCI A allele and/or the APOE4 allele tend to have an earlier age of onset of the disease than female patients.

Age of Onset↗

Effects of two ultrasound devices and angles of application on the temperature of tissue phantom.

Ultrasound is a commonly used therapeutic modality for which there is little research on the effect of treatment dosage on the extent of tissue heating. The purpose of this study was to investigate the effect of two different ultrasound devices, angle of ultrasound application, and treatment time on the temperature of a tissue phantom. Four trials were performed at four ultrasound application angles (90 degrees, 80 degrees, 70 degrees, and 60 degrees) with both an Excel UltraMax and a Mettler Sonicator 720. A continuous 1-MHz frequency at 2.0 W/cm2 was administered for 5 minutes, with tissue phantom temperature recorded at 1-minute intervals. The analysis of the data revealed significant main effects between ultrasound devices, angle of application, and treatment times. Interactions were identified between ultrasound device and treatment times and angle of application and treatment times. Analysis of simple effects revealed significant differences between ultrasound devices after 2, 3, 4, and 5 minutes of treatment and between treatment times and 80 degrees and 60 degrees angles of application. Maximal temperature increase after a 5-minute treatment was 2.025 degrees C. This level of tissue heating falls below expected values and may not yield therapeutic results. The thermal effects were noted to be greatest at 80 degrees and 90 degrees angles of application. Despite appropriate calibration and identical treatment protocols, the two ultrasound devices yielded significantly different tissue phantom temperatures, which were notably lower than expected values. We concluded that direct monitoring of ultrasound device output and calculation of treatment dosage should occur on a routine basis, as treatment outcome will certainly be affected by the actual dosage of ultrasonic energy.

Evaluation Studies as Topic↗

Effects of non-steroidal anti-inflammatory drugs on isolated human polymorphonuclear leukocytes (PMN): chemotaxis, superoxide production, degranulation and N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP) receptor binding.

1. We have examined the effects of tolmetin and meclofenamate on isolated human PMN functions under FMLP stimulating conditions. 2. In a dose dependent manner, tolmetin and meclofenamate inhibited all PMN functions, except that tolmetin stimulated PMN chemotaxis. 3. Meclofenamate was much more potent than tolmetin as an inhibitory agent. 4. We also conducted competitive receptor binding assays for tolmetin, meclofenamate and ibuprofen on the FMLP receptor. 5. All three NSAID inhibited FMLP binding in a dose dependent manner with the potency order being meclofenamate greater than ibuprofen greater than tolmetin.

Adult↗