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Biomedical subjects

J Sherma

Publications and source records attributed to J Sherma.

52 records · Page 3Linked to original sources
PubMedDate not supplied

Effects of restricted food intake on neutral lipid and free fatty acid levels in the digestive gland-gonad complex and faeces of Biomphalaria glabrata (Gastropoda).

High-performance thin-layer chromatography (HPTLC) was used to determine neutral lipids and free fatty acids in the digestive gland-gonad complex (DGG) and faeces of Biomphalaria glabrata. Three populations of B. glabrata were given varying food intakes for one week. They were either unrestricted (lettuce ad libitum plus a weekly feeding of Tetramin), restricted (15-17 mg of lettuce every two days), or starved (no food). Samples were prepared by extraction in chloroform:methanol (2:1), followed by a Folch wash (0.88% aqueous KCl). The major lipids detected in the DGG were triacylglycerols, free fatty acids and free sterols. Free fatty acids and free sterols were the major lipids detected in the snail faeces. Analysis by TLC-densitometry showed a statistically significant difference in the DGG levels of triacylglycerols and free sterols between the unrestricted and restricted groups, as well as between unrestricted and starved groups. Analysis of faecal samples revealed a significant difference in free fatty acids between unrestricted and starved groups on the seventh day.

Animals↗
PubMedDate not supplied

Recent advances in thin-layer chromatography of pesticides.

Advances in the applications of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC) for the separation, detection, and qualitative and quantitative determination of pesticides, other agrochemicals, and related compounds are reviewed for the period 1998-2000. Analyses are covered for a variety of samples, such as food, biological, and environmental, and for residues of pesticides of various types, including insecticides, herbicides, and fungicides, belonging to different chemical classes. References on formulation analysis, hydrophobicity studies, and the use of TLC and thin-layer radiochromatography (TLRC) for studies of pesticide metabolism, degradation, uptake, and related studies are also included.

Chromatography, Thin Layer↗
PubMedDate not supplied

Pesticide residue analysis (1999-2000): a review.

The literature of pesticide residue analysis is reviewed selectively for the period 1999-2000. Analyses of a wide range of pesticide classes and sample types, as well as some related organic pollutants, are covered. Studies of exposure and monitoring as well as pesticide degradation, persistance, leaching, mobility, and metabolism studies are included.

Chemistry Techniques, Analytical↗
PubMedDate not supplied

Practical screening procedure for sulfathiazole in honey.

A simple, inexpensive procedure is described for rapidly screening small samples of honey for sulfathiazole (ST), a drug formerly used but not approved in the United States for the prophylactic treatment of American foulbrood disease of bees. The method uses 2 plastic tubes arranged in tandem. The upper tube contains a bed of alumina, which removes some interfering pigments. The lower tube contains a very small bed of anion exchange resin in the HSO4- form, which traps the ST. The drug is eventually eluted and detected using the Bratton-Marshall diazotization-coupling reagents. Honey containing 0.1 ppm ST can be readily detected. An optional dye concentration step permits the detection of as little as 25 ppb ST.

Chromatography, Ion Exchange↗