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J Shuttleworth

Publications and source records attributed to J Shuttleworth.

23 records · Page 2Linked to original sources
PubMed1981

Low temperature treatment of Namalwa cells causes superproduction of interferon.

Interferon production in Namalwa cells, a human lymphoblastoid line, was enhanced by lowering the incubation temperature after induction. The optimum conditions for this effect were established. At the lower temperature interferon synthesis proceeded at a lower rate but continued for longer. Interferon mRNA was shown to be associated with the polysomes for longer after induction. Addition of drugs that inhibit transcription did not prevent the increased production of interferon. Thus, the increased production of interferon is due to the prolonged translation of interferon mRNA.

Cell Line↗
PubMed1979

Interferon production: variation in yields from human cell lines.

A survey of human diploid, aneusomic, transformed and tumor fibroblast or fibroblastoid cell lines for their capacity to produce interferon after polyriboinosinic acid:polycytidylic acid induction showed considerable variation in responsiveness. There was no apparent correlation between karyotype or phenotype and interferon production. Pretreating or "priming" the cells with human interferon generally led to increased yields of interferon after polyriboinosinic acid:polycytidylic acid induction in all cell lines tested. All the cells also showed the "super-induction" phenomenon, although to varying degrees. The combination of priming and superinduction conditions led to the production of very high yields of interferon in some cell lines, but in other lines, yields less than either the primed or superinduced amounts were found. A more limited survey of human cell lines for their capacity to produce interferon after Newcastle disease virus induction also showed that yields varied from line to line. However, there was little evidence to suggest that ability to produce interferon after Newcastle disease virus induction correlated with that after polyriboinosinic acid:polycytidylic acid induction.

Cell Line↗