Biomedical subjects
J Siwik
Publications and source records attributed to J Siwik.
[Althesin anesthesia in laryngeal microsurgery].
Explore the source record for details and available documents.
The effect of bone cement used for endoprosthesis stabilization on the activity of lysosomal enzymes in peripheral blood granulocytes.
The effect of bone cement used for stabilization of hip joint endoprosthesis on the activity of lysosomal enzymes in peripheral blood granulocytes was studied. The operations were carried out under balanced, non-toxic, endotracheal anaesthesia using muscle relaxants. The blood for tests was obtained from an antecubital vein before and during anaesthesia, before application of bone cement, and 10 min., 1 hour and 24, 48 and 72 hours after endoprosthesis implantation using bone cement. The activity of acid phosphatase, acid protease and neutral protease was determined by calculating it in relation to protein level in the studied fractions. During anaesthesia as well as during the operation when bone cement was applied no statistically significant differences were found in the activity of lysosomal enzymes. On the other hand, after the operation statistically significant changes were found in the activity of lysosomal marker enzymes. Lability of lysosomal membranes appeared with permeation of hydrolases into supernatant. These results confirmed the importance of lysosomal proteins in the processes of wound healing and general postoperative course.
[So-called mirror injuries in severe head trauma].
Severe head traumas cause high mortality. It was the reason to analyze such a group of patients, treated in the Neurosurgical Department of the CCH MMA in the years 1980-1990. All cases of mirror injuries were divided into three groups. The lesions of frontal and temporal lobes present brain-stem lesion like syndromes. Personality disorders were associated with lesions of both frontal lobes. The third syndrome is caused by focal lesions according to their location. The authors discuss medical and surgical treatment and stress the importance of early brain protection.
Effect of endotracheal anaesthesia on the activity of lysosomal enzymes in peripheral blood granulocytes.
The purpose of this study was to assess the effect of endotracheal anaesthesia on the activity of lysosomal enzymes in peripheral blood granulocytes. The determinations were carried out before anaesthesia, after 1 hour of anaesthesia and 1 hour after awakening. The activity of lysosomal enzymes was assessed on the basis of the activity of marker-enzymes, i.e. acid phosphatase, acid protease and neutral protease. The lysosomal fraction was prepared by Choduker's method and the activity of lysosomal enzymes was calculated for 100 mug of protein. The obtained results showed a statistically not significant rise in the activity of these enzymes in the lysosomes during anaesthesia without a similar rise in the supernatant. The activity of marker enzymes in lysosomes rose to the initial values after the awakening of patients. Such rapid changes in the activity of lysosomal enzymes seem to depend mainly on the mobilization of the reserve pool of granulocytes from other sources than the peripheral blood and, to a lesser extent, on latency and stabilization of lysosomal membranes by anaesthetic agents used in correctly conducted anaesthesia.