[Infections by Listeria monocytogenes. 15 years' experience].
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Adult↗
Biomedical subjects
J Smayevsky
Publications and source records attributed to J Smayevsky.
[Sacroileitis caused by Staphylococcus aureus].
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Adolescent↗
[Specificity against group A, C, and G streptococci of the reverse CAMP test for identifying Clostridium perfringens].
We studied "reverse CAMP" test specificity for the identification of Clostridium perfringens, and the probable existence of cross-reactions with groups A, C and G streptococci. Ninety eight Clostridium strains were tested with ten S. agalactiae, ten S. pyogenes, two group C streptococci and one group G. All the strains of S. agalactiae yielded a positive "reverse CAMP" reaction when it was performed with C. perfringens strains. Cross-reactions were not detected when C. perfringens were tested with groups A, C or G streptococci. The "reverse CAMP" test proved to be specific for all the C. perfringens strains used.
Animals↗
[Infection of a cerebrospinal fluid shunt system by Bacillus circulans and Bacillus larvae].
A two episodes case of CSF ventriculo-atrial shunt infection due to B. circulans and B. larvae is presented. B. circulans was first isolated from 4 blood cultures and CSF (shunt valve tap). The patient showed a brain damage syndrome reversible with antibiotic treatment. Lethal toxin production was demonstrated for the B. circulans strain in a mouse model. This strain was found to be a variant of Gordon's description as it produced urease and was tolerant to 7% NaCl. The patient recovered after cefotaxime, cotrimoxazole and rifampicin treatment. A second infection due to B. larvae was detected two months later. The shunt system was removed due to obstruction and a scanning electron microscopy study was performed. Confluent masses of white blood cells and rods were observed on the inner surface of the catheter. As for as we know, this is the first case of human infection due to B. larvae.
Adolescent↗
[Clostridium difficile diarrhea: frequency of detection in a medical center in Buenos Aires, Argentina].
Clostridium difficile has been recognized as the most important enteric pathogen of nosocomial antibiotic-associated diarrhea (CDAD) in adults from industrialized countries. The importance of C. difficile as a cause of diarrhea in ambulatory patients appears underestimated or under-recognized. Since the 1980's, outbreaks of CDAD have been increasingly reported, but there are few data available in Argentina. We developed a retrospective study to provide some information about CDAD in our country. From July 1998 to November 1999, a total of 245 fecal specimens from hospitalized and some ambulatory patients were tested in order to confirm the diagnosis of CDAD. C. difficile cytotoxin (toxin B) was identified by detecting its cytopathic effect on monolayers of McCoy culture cells. For culture and isolation of C. difficile, stool samples were prepared by ethanol shock prior to plating onto a selective medium which contained blood, cefoxitin and fructose. Of the 245 samples, 14 (5.8%) were identified as positive by the cell cytotoxicity assay. Using the criteria of isolation of cytotoxigenic C. difficile positivity increased to 6.5% (16 samples). Thirteen of the positive results were from hospitalized patients (81.3%) and 3 (18.7%) from outpatients. The mean age of inpatients was 72.9 years (ranging from 47 to 88). All patients had received 2 or more antimicrobial agents (most of them beta-lactams) 2 months before the appearance of diarrhea. There was one patient who had received only chemotherapy. The prevalence of CDAD in this study was less than in others previously reported. This difference may be due to the fact that not all general practitioners include testing for C. difficile when the patient with diarrhea had previously received antibiotics. More educational programs should be directed to all physicians, concerning the role of C. difficile as an important enteric pathogen in patients who have undergone treatment with antimicrobial or chemotherapeutic agents.
Adult↗
Modified Spot CAMP Test: A rapid, inexpensive and accurate method for identification of group B streptococci.
A rapid modified spot CAMP test using 183 clinical isolates of beta haemolytic streptococci was compared with the standard CAMP test described by Christie et al. The scheme of biochemical identification and serological confirmation was taken as reference method. The sensitivity of both tests was 100%, and the specificity of the rapid and standard tests was 96.8% and 88.9% respectively. The modified spot CAMP test is a rapid, inexpensive and accurate method for the identification of group B streptococci, and is more specific than the standard CAMP test.
Agar↗
[Microbiologic study of bacteremia and fungemia in chronic hemodialysis patients].
Microbiologic study of bacteremia and fungemia in chronic hemodialysis patients. Bloodstream infections are the second cause of death in patients in chronic hemodialysis (CHD), and the knowledge of the epidemiology is useful to establish proper empiric therapies. The aim of this study was to evaluate the frequency and distribution of microorganisms, in bacteremia and fungemia in 530 patients in CHD. Two hundred and forty eight blood culture series from 114 patients with suspected bacteremia were processed; 44% of them were positive from which 71% (n=78) were clinically significative and belonged to 58 patients. Sixty eight percent of these isolates were gram-positive cocci (n:53), and 22% gram-negative rods (n:17). Staphylococcus aureus was the most prevalent pathogen showing 23% of methicillin-resistance. Candida spp. was the fourth pathogen most common in frequency.
Argentina↗
[Microbiological diagnosis of infections by coagulase-negative staphylococci].
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Animals↗
[Non-pigmented Serratia: utility of hydrolysis of Tween 80 in its identification].
The ability of Serratia sp. to hydrolyze Tween 80 and the usefulness of this test for differentiating it from other Gram negative bacilli was studied, comparing it with the capacity of production of DNAsa by the same strains using the O-Toluidine blue method. The total number of strains assayed was 88. The sensitivity obtained was 83.9% and specificity 91.3%, with a positive predictive value of 83.9% and a negative predictive value of 91.2%. Using this method to differentiate only non-pigmented Serratia from Klebsiella sp. and Enterobacter sp. the sensitivity and specificity obtained was 100%. Concerning the cost, this method proved to be 3.86 times cheaper than the DNAsa one.
DNA, Bacterial↗
[Clinical, epidemiologic, and microbiologic study of urinary infection in patients with renal transplant at a specialized center in Argentina].
Sixty-four patients were evaluated for urinary tract infection (UTI) during the first six months after renal transplantation. Eighty per cent received the allograft from a living, related donor and 37% were female. The median age was 33.8 years. All the patients received cefazolin as perioperative antibiotic prophylaxis. No prophylaxis was given during the bladder catheterization period, which had a median duration of 5.9 days. Nineteen per cent developed predisposing urological factors (PUF). The incidence of UTI was 41% and 15% of all these patients developed bacteremia from this source. UTI was observed in 50% of the patients without PUF between the 5th and 12th days post-transplantation, and these cases had been detected by the control urine culture performed when the bladder catheter was withdrawn. This group of patients accounted for 14% of all the patients. Recurrence was present in 42% of the patients with UTI (19% had reinfection, 4% relapse and 19% both). They were more frequent in patients with PUF than in the patients without; 78% vs. 24%, respectively (p < 0.05). The overall UTI incidence was also higher; 75% vs. 33%, respectively (p < 0.05). The same was observed for the median number of episodes (3 vs. 1). The organisms most frequently isolated were E. faecalis (21%). E. cloacae (17%), and E. coli (17%). Species prevalence differs from that of other reports, probably due to the different institutional antibiotic management. Since half of the UTI episodes suffered by patients without PUF were associated to bladder catheterization, there is a need to institute antibiotic prophylaxis in our center.
Adolescent↗
[Utility of pyrrolidonyl-arylamidase detection for typing Enterobacteriaceae and non-fermenting Gram-negative bacteria].
Detection of pyrrolidonyl-aryl-amidase activity (PYR) is an important tool to identify gram-positive cocci, such as staphylococci, enterococci, streptococci, and other related genera. However, only few studies evaluating its usefulness with gram-negative rods have been published. Thus, a prospective study including 542 and 215 unique clinical isolates of Enterobacteriaceae and non-fermentative gram-negative rods, respectively, was undertaken. Strains were identified by conventional methods. PYR test was performed using a commercial kit, according to the manufacturer recommendations. Positive results were uniformly obtained for the PYR test with the following species: Citrobacter spp, Klebsiella spp, Enterobacter aerogenes, Enterobacter agglomerans group, Serratia marcescens and S. odorifera. On the other hand, negative results were uniformly displayed by E. coli (including inactive E. coli), Protease group, Salmonellia spp, Shigella spp, Acinetobacter spp, Burkholderia (Pseudomonas) cepacia and Flavobacterium spp. Variable results were shown in Pseudomonas aeruginosa, Stenotrophomonas (xanthomonas) malthophilia, Kluyvera cryocrescens, and Enterobacter cloacae. PYR test proved to be a reliable and simple tool to rapidly distinguish certain species belonging to Enterobacteriaceae (ie. Citrobacter freundii from Salmonella spp, and inactive E. coli from K. ozaenae). Further studies, including a wide diversity of species, are required to assess usefulness of the PYR test for the identification of non-fermentative gram-negative rods.
Aminopeptidases↗