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J Smrt

Publications and source records attributed to J Smrt.

34 records · Page 2Linked to original sources
PubMed1984

Stability of (2'-5')oligoriboadenylates in various sera.

Avian and mammalian sera were found to contain an enzyme activity degrading 2-5A oligonucleotides. The most extensive degradation of the A2' p5' A was observed in chicken serum. Degradation of this compound is not affected by the presence of cAMP, dsRNA, Mg2+, but is significantly inhibited by EDTA. The enzyme activity described is not inactivated by heating to 56 degrees C for 30 min. The 5-mU3' p5' A has also been degraded in chicken serum.

Adenine Nucleotides↗
PubMed1983

Antiviral and anticellular effects of synthetic (2'-5')-oligoadenylate (A 2' p 5' A 2' p 5' A) in Rauscher murine leukaemia.

Antiviral and antileukaemic effects of the synthetic (2'-5')-oligoadenylate trimer [(2'-5')-ApApA] were demonstrated in BALB/c mice infected with Rauscher murine leukaemia virus (RMLV) by intraperitoneal (i.p.) treatment for 5-20 days (100 micrograms--1 mg daily doses) as evidenced by 72% suppression of viraemia and by decreased activity of serum reverse transcriptase levels. Electron microscopy revealed more than 95% inhibition of RMLV replication as compared to controls in transformed spleen cells from mice treated 5 times with 1 mg dose of (2'-5') ApApA. A significant and dose-dependent reduction of spleen weights of the RMLV-infected mice treated with (2'-5') ApApA was also observed. The antileukaemic effect of (2'-5-') ApApA was enhanced by simultaneous i.p. injection of amphotericin B (20 micrograms/mouse). In comparison to the effect of interferon (IFN) on RNA tumour viruses, our results suggest a higher antiviral activity of the synthetic (2'-5') ApApA oligonucleotide in suppressing RMLV replication in vivo.

Adenine Nucleotides↗
PubMed1977

Enzymes relevant to cancer chemotherapy II. Heterogeneity of rat kidney uridine kinase following chromatography on Sepharose 6B coupled to N4-(5-amino-Pentyl)-cytidine.

Using Sepharose 6B coupled to N4-(5-aminopentyl)-cytidine at least three different peaks of rat kidney uridine kinase activity have been found. Prior to affinity chromatography the enzyme was partially purified by ammonium sulfate precipitation. The elution was carried out with 0.15 M NaCl or with 0.012 M ATP. The importance of different forms of uridine kinase in eukaryotic cells for cancer chemotherapy with uridine and cytidine analogues is discussed.

Animals↗