Biomedical subjects

J Soltýs

Publications and source records attributed to J Soltýs.

8 recordsLinked to original sources

PubMed1998

Development and morphological variability of Echinococcus granulosus.

Characteristics are presented of Echinococcus granulosus strobila from dogs experimentally infected with protoscolices from a single cyst isolated from the liver of a domestic pig in a central Slovakian district. Adult cestodes are characterized mainly by 28-32 hooks highly variable in shape and size; 26-51 testes situated mostly throughout the proglottis (1 or 3 rows behind the vitelline gland); a lobate ovary; the shape of the uterus, which in 10% of proglottides is more or less spherical, in 62% is saccular with lateral sacculations of variable size, in 27% is saccular without lateral sacculations and in 1% is more or less tubular or strongly ramified; and an intermediately rapid rate of development lasting until between 40 and 44 days p.i. The present report analyzes the high degree of variability of the clone studied, referring to the knowledge on the characteristics of the pig, sheep, and cattle strains of E. granulosus and E. multilocularis species, with which some taxonomic characters of the clone representatives overlap.

Animals↗

PubMed1997

Effects of concurrently administered copper and mercury on phagocytic cell activity and antibody levels in guinea pigs with experimental ascariasis.

The subchronic effect of copper and mercury on selected immunological parameters in guinea pigs with experimental ascariasis was studied. Cupric sulphate and mercuric chloride administered for 28 days did not suppress the levels of specific circulating antibody in a subsequent Ascaris suum infection. Intoxication of animals significantly inhibited the phagocytic activity of peritoneal macrophages throughout the experiment. Ascaris suum infection elevated macrophage phagocytic activity but values remained significantly lower in comparison with infected non-intoxicated animals. Both the concurrently administered metal compounds changed the phagocytic ability of polymorphonuclear leucocytes in the blood. The intensity of infection assessed by the number of Ascaris larvae migrating in the lungs of animals currently intoxicated with both the metal compounds increased by 28.6%, compared with controls.

Animals↗

PubMed1996

[The effect of copper on immune responses in guinea pigs with experimental ascariasis].

A subchronic effect of copper on selected immunological parameters was studied in guinea pigs with experimental ascariosis (Ascaris suum). CuSO4 given orally for 28 days moderately increased T and B cells in the animal lymphoid organs. CH50 and AH50 complement levels remained unchanged during CuSO4 administration. A subsequent infection of subchronically intoxicated guinea pigs caused a repeated insignificant stimulation of B cell values while the percentage of T cells was not increased. On the contrary, the complement levels increased significantly in intoxicated infected animals, namely CH50 levels from day 5 to day 15 and AH50 levels from day 1 to day 7 post infection. No statistically significant differences were observed in comparison with infected non-intoxicated guinea pigs. The lean intensity of infection evoked by A. suum larvae migrating in the lungs of intoxicated animals was 29% lower than in control.

Animals↗

PubMed1994

Immunorestorative effect of glucan immunomodulator on guinea pigs with experimental ascariosis.

The immunorestorative effect of glucan immunomodulator, combined with porcine immunoglobulin and zinc (GI) on T- and B-lymphocytes and peritoneal macrophage phagocytic ability was studied in guinea pigs with experimental ascariosis (Ascaris suum) after a cyclophosphamide (CY)-evoked immunosuppression. During the migration phase of A. suum infection GI exerted a significant restorative effect on the CY-reduced percentage occurrence of T- and B-cell populations in the mesenteric, mediastinal and hepatic lymph nodes and spleen of A. suum hosts. On the contrary, it did not influence the CY-suppressed phagocytic activity and index of phagocytic activity of the peritoneal macrophages. The protective effect of the GI evaluated by the reduction in the number of migrating ascarid larvae in the lungs of guinea pigs after immunosuppression with CY and administration of GI was 14.46% higher, compared with the suppressed and infected group without administration of GI.

Adjuvants, Immunologic↗

PubMed1994

Effect of heavy metal emission on the immune response of sheep with experimental fasciolosis.

The effect of industrial heavy metal emission on immune reactions was studied in lambs subsequently infected with Fasciola hepatica. The heavy metal emission (Cu, Hg, Pb, Cd, Cr, Zn) was collected from grass at 5 km distance from a mercury plant. Administration of the emission for 28 days prior to infection of the animals reduced the percent circulating T- and B-cell populations when compared with untreated animals and animals infected with F. hepatica only. The administration of emission had no suppressive effects on the levels of circulating antibodies to F. hepatica following infection. The intensity of liver infection with mature F. hepatica in the emission-fed and infected lamb group increased by 21.5%. This difference in comparison with control infected animals was not significant.

Air Pollutants↗

PubMed1993

Effects of heavy metals imission on the cellular immunity of guinea pigs with experimental ascariosis.

A study was performed on the short-term effects of industrial heavy metals imission (Hg, Cu, Pb, Zn), collected at 5 km from a mercury-producing plant, on T- and B-lymphocytes and peritoneal macrophages of guinea pigs with experimental ascariosis (Ascaris suum). Administration of the imission for 5 days showed a significant decrease in T- and B-cell population and in macrophage phagocytic ability during the migration phase of Ascaris suum when compared with an infected but untreated group of animals. This was also manifested by an eight-fold increase in the density of migrating ascaris larvae in the lungs. Macrophage metabolic activity in animals given imission was significantly suppressed only until Day 7 post-infection. The most striking decrease in the immunological responses was observed in guinea pigs that were given imission but were not infected.

Air Pollution↗

PubMed1992

Effect of glucan preparation on immunocompetent cells and phagocytic ability of blood leucocytes in experimental ascariosis of pigs.

In experimental ascariosis (Ascaris suum) of pigs a preparation of glucan combined with pig immunoglobulin and zinc was found to significantly elevate the levels of both T and B lymphocytes in the blood, and to significantly stimulate the phagocytic ability of monocytes and neutrophilic granulocytes in experimental pigs. The protective effect of the preparation, assessed by the reduction in the number of migrating ascarid larvae in the lungs of pigs was 65%.

Animals↗

PubMed1992

[Immunostimulatory effects of certain substances in experimental ascaridiasis in pigs].

The effects were studied of four immunomodulative substances of biological origin (purified glucan, glucan with porcine immunoglobulin and zinc, transfer factor and vitamin A) on T and B lymphocytes, along with their protective effects in pigs with experimental ascariasis. These substances activated T and B lymphocytes with different intensity. The nonspecific stimulation of these cells in the blood of pigs with the immunomodulative substances intensified the protective mechanisms; this resulted in a decreased number of migrating ascaris larvae in the lungs of pigs by 17% to 65%. Of the four immunomodulative substances studied, transfer factor and glucan-based substances with porcine immunoglobulin and zinc proved to be the most effective. Both the substances exerted a significant stimulative effect on T- and B-cell populations even in the first days after infection, reaching the maximum on days 7-9 p. i. (Figs. 1 and 2). The protective effect, assessed by the reduction of migrating ascaris larvae in the lungs of pigs, was 62% and 65% using transfer factor and glucan preparation with homologous immunoglobulin and zinc, respectively. The percentage of occurrence of T and B lymphocytes, obtained by the use of purified glucan and vitamin A, was not in correlation with their protective effect (purified glucan 17%, vitamin A 50%). The immunomodulative effects of little effective purified glucan increased when combined with porcine immunoglobulin and zinc. The results are significant from the aspect of the nonspecific stimulation of immunity in ascariasis and in some other helminthoses.

Animals↗