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Biomedical subjects

J Stencl

Publications and source records attributed to J Stencl.

At least 37 records · Page 2Linked to original sources
PubMed1986

Evidence of Chlamydia trachomatis infection in gynaecological patients.

Direct microscopic examination of cervical smears taken from gynaecological patients and stained by Giemsa and Gimenez revealed the presence of chlamydial inclusions in 2 and elementary bodies in 6 out of 40 samples tested. Attempts of demonstration of chlamydiae from cervical smears of further patients in chick embryo yolk sacs and cycloheximide-treated McCoy cells using the centrifugation of infectious inocula succeeded in 4 and 9 cases of 71 patients examined, respectively. One isolated strain behaved as Chlamydia trachomatis based on its sensitivity to sulfadiazine, absence of killing the intraperitoneally inoculated mice, demonstration of its inclusions by iodine staining and by detection of its elementary bodies by immunofluorescence technique using mouse serum directed to C. trachomatis but not C. psittaci serotypes. At the same time, 11 of 68 patient sera tested by microimmunofluorescence test contained IgG antibodies to genitourinary serotypes of C. trachomatis, suggesting the previous contact of patients with sexually-transmitted chlamydiae. The results obtained indicate that chlamydiae may pose a public health problem in women of sexually active age also in Czechoslovakia.

Adult↗
PubMed1983

Human cell surface proteins selectively assembled into vesicular stomatitis virus virions.

Vesicular stomatitis virus (VSV) selectively assembled proteins from human cells into progeny virions. These proteins can be surface labeled before infection with 125I, and when purified virus was examined by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, only two or three bands of proteins (Mr around 100K) were seen. Antisera to these proteins were produced, using as immunizing antigen VSV tsO45 mutant, defective in assembly of G protein, which had been made at the nonpermissive temperature in the three human tumor cell lines, HeLa (cervical carcinoma), T47D (breast carcinoma), and HMB2 (melanoma). After absorption with wild-type VSV, each of the antisera displayed a different pattern of reactivity; at least three antigenic specificities were detected. Two of them, corresponding to antigens selected by VSV from HeLa and T47D, were to some extent related and they showed an association mainly with epithelial cell-derived gynecological tumors, but they were absent in carcinomas of lung or of digestive tract. These (or related) antigens were expressed in a lower level in some normal tissues, mainly in ovaries. Antigen(s) assembled by VSV from the melanoma cell line was entirely different and appeared to be associated with cell growth. The grounds for selective assembly of these specific proteins by VSV are not clear; they either share with viral surface glycoproteins some physical or structural properties, which are critical for incorporation into the viral envelope, or conceivably they even may represent uncleaved precursor proteins coded by env genes of incomplete genomes of endogenous human retroviruses.

Antigens, Neoplasm↗