Deafness as the presenting sign of posttraumatic pneumococcal meningitis.
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Child↗
Biomedical subjects
J Stephens
Publications and source records attributed to J Stephens.
Can group- and serovar-specific proteins be detected in Ureaplasma urealyticum?
Ureaplasma urealyticum has been subspeciated by a number of serologic methods. Eight serotypes have been identified by modified metabolic inhibition, growth inhibition and indirect hemagglutination. Fourteen serovars have been identified by immunofluorescence and 16 by the mycoplasmacidal assay. The present studies were performed to determine if group-specific antigens could be detected by immunofluorescence and if group- or serovar-specific antigens could be detected by enzyme-linked immunosorbent assay and immunoblotting. Reaction of rabbit antisera to U. urealyticum with homologous and heterologous serotypes based upon end point immunofluorescent titration did not differentiate the serovars into the two biotypes. In attempts to quantitate the number of a given serovar present in clinical specimens, the total number of colonies that were stained often exceeded 100%, suggesting either that the serovars represented in stock cultures are not truly representative of those present in humans or that certain strains express multiple serovar specificities. End point titration in an enzyme-linked immunoadsorbent assay using either whole cells or cell lysates and rabbit antisera failed to distinguish between group and serovars (i.e. in many cases end point titers were only 2-fold lower with heterologous antigens). Furthermore convalescent sera from patients infected with a single serovar failed to induce a serovar-specific response detectable in an enzyme-linked immunosorbent assay. Likewise immunoblotting of one dimensional electrophoretograms with sera from humans known to be infected with a single serovar showed that antibodies against that serovar recognized peptides present in all serovars and reacted with those bands with various degrees of intensity.(ABSTRACT TRUNCATED AT 250 WORDS)
Antigens, Bacterial↗
HER-2 and cancer antigen 125 evaluation in ovarian borderline tumors by immunohistochemistry and fluorescence in situ hybridization.
The study determined the expression of cancer antigen (CA) 125 and HER-2 in 45 borderline ovarian tumors (BOTs) and investigated the correlation of these biologic markers with histologic type, clinical stage, and outcome. The level of CA 125 protein was assessed using DAKO's M-11 clone antibody in immunohistochemistry (IHC) assays (Carpinteria, CA). The HER-2 protein expression was assessed in IHC assays using the HercepTest (DAKO), and the HER-2 gene copy number per cell was investigated through fluorescence in situ hybridization (FISH) assays using VYSIS' PathVysion DNA Probe (Downers Grove, IL). Expression of the CA 125 protein was detected in 49% of the samples (22 out of 45 tumors) and significantly associated with the serous histologic type. However, CA 125 expression did not associate with clinical stage or outcome. Protein overexpression or gene amplification of HER-2 was not found. However, abnormal FISH results were detected in 16% (seven out of 45 patients) of specimens comprising extranumerary copies of HER-2 and/or chromosome 17 per cell. Abnormal FISH results were found to be independent of CA 125 expression and histologic type whereas they positively associate with advanced clinical stage. Our data show that HER-2 is not altered in BOTs, and the presence of aneusomy for chromosome 17 and HER-2 may predict tumor progression.
Adenocarcinoma, Mucinous↗
Fine needle aspiration cytology of mycobacterial spindle cell pseudotumor. A case report.
We report a case of mycobacterial spindle cell pseudotumor (MSP) in a lymph node from an acquired immunodeficiency syndrome patient diagnosed by fine needle aspiration (FNA). The FNA cytology was characterized by spindle cell proliferation without the typical foamy histiocytes usually seen in mycobacterial infections and mimicked a mesenchymal neoplasm, particularly Kaposi's sarcoma. This case illustrates the importance of including MSP in the differential diagnosis of lymph node FNAs from immunocompromised patients, particularly those that show spindle cell proliferation suspicious for Kaposi's sarcoma or another mesenchymal neoplasm.
Acquired Immunodeficiency Syndrome↗
An in vitro investigation of the efficacy of CPC for use in toothbrush decontamination.
PURPOSE: A product designed as a toothbrush disinfectant containing cetylpyridinium chloride (CPC), a quaternary ammonium compound, recently was introduced. The purpose of this study was to provide additional evidence that CPC provides a practical solution for destroying residual microorganisms on air-dried toothbrushes and toothbrushes stored in a travel container. METHODS: Sterile synthetic toothbrushes were inoculated with optical density standardized laboratory cultures of Staphylococcus epidermidis or Candida albicans. Half were then disinfected with CPC and half were used as untreated controls. The toothbrushes were vortexed in sterile saline solution, diluted in a ten-fold series, and plated on 5% blood agar or Sabouraud dextrose agar. The plates were incubated at 37 degrees C in a normal atmosphere for 48 hours, and colonies were counted. RESULTS: CPC produced significant decreases in residual microorganisms. Using the CPC spray treatment on air-dried toothbrushes, Staphylococcus epidermidis essentially was reduced 100-fold, while Candida albicans had a 94% reduction of growth. Bacterial counts were higher in the samples stored in closed containers as compared to the air-dried samples. CONCLUSION: CPC appeared to be an effective toothbrush disinfectant for the organisms evaluated. It is practical and economical. CPC could easily fit into the recommendations of a practice committed to infection control.
Anti-Infective Agents, Local↗