Biomedical subjects

J Stingl

Publications and source records attributed to J Stingl.

At least 19 recordsLinked to original sources

PubMed1996

Selective growth of freshly isolated human breast epithelial cells cultured at low concentrations in the presence or absence of bone marrow cells.

In this study, we show that conditions previously found to promote the selective growth of human breast epithelial cells (HBEC) in serum-free primary cultures established from normal or malignant tissue can be extended to cultures initiated at low seeding densities (< 5000 cells/cm2). The epithelial nature of the cells produced was documented by their positive staining with antibodies specific for keratins 8, 14, and 18, and 2 antibodies that recognize epithelial-specific antigens (Ber-EP4 and HB8630). HBEC growth was not affected, either positively or negatively, by the use of a medium containing a combination of fetal calf and horse serum, which promotes the growth of many types of stromal cells and associated hematopoietic precursors, or by the inclusion in the initial cell suspension of marrow cells at HBEC to marrow cell ratios typical of bone marrow samples from patients with metastatic breast cancer. The presence of fibroblast feeders from a variety of sources enhanced the growth of HBEC to different degrees. In cultures initiated with low numbers of cells obtained from samples of breast carcinoma, HBEC growth was generally reduced by comparison to cultures of normal HBEC. With the detection methods used, it was not possible to determine the extent to which this decreased growth was due to a reduced frequency of malignant HBEC with in vitro precursor activity, or the presence of reduced numbers of residual normal HBEC precursors, or both. However, preliminary data indicate that this approach also allows the detection of some breast carcinoma cells with proliferative ability that are present in the marrow or pleural effusions of some breast cancer patients. These studies demonstrate the feasibility of detecting normal and malignant HBEC with growth potential when these are cultured at low density and/or as rare contaminants of marrow cell suspensions, and provide a starting point for their further characterization.

Bone Marrow Cells↗

PubMed1995

[Variations in extrahepatic bile ducts, their arteries and pancreatic ducts].

The author presents a brief account of congenital varieties of extrahepatic biliary pathways, a cystica and pancreatic ducts and explains their development. Good knowledge of these varieties is of great importance for detailed preparation in laparoscopic cholecystectomy and during fibroscopic examination of duodenal papillae.

Arteries↗

PubMed1994

Early postnatal growth of skeletal muscle blood vessels of the rat.

The development of blood vessels during the first three postnatal weeks was studied in the ventral stripe of the spinotrapezius muscle of the rat by use of India ink-gelatine injections, and electron microscopy. The number of terminal arterioles and collecting venules remained unchanged postnatally in the observed area. A remarkable proximodistal gradient of vascular development was apparent: while the basic structure of the hilar vessels remained unchanged in the time studied, the intramuscular arteries and veins matured gradually. More peripherally, gradual maturation of terminal and precapillary arterioles was observed. The capillary endothelium and the pericytes showed immature features, and remained unchanged during the time studied. An intense rebuilding activity was found in the endothelial cells of the growing venules, expressed by various forms of gaps, covered by an intact basal lamina and pericytes. Numerous mast cells and macrophages were found along all vessels. Intramuscular lymphatics were not present prior to the first postnatal week.

Animals↗

PubMed1994

Ontogenesis of the vascular bed in the human skeletal muscles.

The ontogenetic development of the vascular bed was studied in the muscles of human fetuses after India ink and gelatine injections. By the 12th week of intrauterine life the architectural arrangement of the largest (primary) intra-muscular vessels is qualitatively comparable with that of the adult muscles. The smaller (secondary) vessels, as well as the microcirculatory bed, have very immature features in all fetal muscles studied. This part of the vascular bed develops gradually during further time from a primitive intramuscular capillary network. The basic architectural arrangement of the microcirculatory bed in adult muscles, and the development of the structure of the hilar vessels of the gastrocnemius muscle were described in the muscles of fetuses, newborns, and adults.

Blood Vessels↗

PubMed1992

In vitro screening of crude extracts and pure metabolites obtained from marine invertebrates for the treatment of breast cancer.

A total of 15 samples (crude extracts and pure secondary metabolites) obtained from marine invertebrates collected from the offshore waters of British Columbia, Papua New Guinea, and Sri Lanka have previously been shown to exert cytotoxic activity in the in vitro L1210 leukemic bioassay. We screened these metabolites for in vitro cytotoxic activity against the drug-sensitive breast-tumor cell lines MCF-7, T-47D, ZR-75-1, and MDA-MB-231; the multidrug-resistant and P-glycoprotein (Pgp)-positive breast-tumor cell lines MCF-7 Adr and MDA-A1r; and normal and malignant human breast epithelial cells (HBEC) in primary culture. Eight samples exhibited significant [drug concentration resulting in a 50% decrease in cell growth as compared with controls (ED50), less than 25 micrograms/ml] dose-dependent cytotoxicity against the drug-sensitive cell lines; the ED50 values were as low as 0.004 micrograms/ml. Five of the eight samples exhibited significant cytotoxicity against the multidrug-resistant cell lines; the ED50 values were as low as 0.0006 micrograms/ml. Incubation of MCF-7 Adr cells with varying concentrations of compounds in the presence of Adriamycin demonstrated that none of the compounds tested interfered with Pgp function. Results obtained using HBEC in primary culture showed a wide range of chemosensitivities for a given drug against tissue taken from different patients, demonstrating the uniqueness of the response of different individuals to chemotherapy.

Animals↗

PubMed1990

[The history of morphological research of the vascular bed of the skeletal muscles].

The present paper briefly reports on the history of vascular research with special emphasis to the contribution of G. Prochaska and W. Spalteholz in the topic of the vascular pattern in the skeletal muscle. The merit of Prochaska was to summarize the findings of earlier investigators and to perform own experiments using injection techniques to demonstrate vessels in the muscle. For a longer period his results has served as the morphological base for the physiological understanding of the muscular circulation. 100 years later W. Spalteholz was the first scientist which has elaborated a complete scheme of the vascular distribution in the muscle including the capillaries, using his special method, to make tissue translucent combined with injection techniques. His fundamental work "Die Vertheilung der Blutgefässe im Muskel" was published in 1888. In many details it is valid up to recent time because the concept of Chambers and Zweifach can not be applied to the circulation of the muscle. Additionally our report gives information of further development of vascular research in the muscle with respects to theoretical problems and practical application in the muscle surgery.

Anatomy↗

PubMed1989

[Normal anatomy of the Achilles tendon].

The paper contains introductory historic and nomenclature comments, survey of topography and basic morphological modifications of Achilles tendon including description of all m. triceps surae components. Morphology of the distal third and tendon insertion, bursa calcanea and arterial supply of both the tendon and its sheaths is described in detail. The paper is concluded by a brief survey of tendon ontogenesis. With respect to its general topic the paper provides morphological basis for topical clinical needs especially in sports medicine and orthopedics.

Achilles Tendon↗

PubMed1987