Biomedical subjects

J Stoltenberg

Publications and source records attributed to J Stoltenberg.

3 recordsLinked to original sources

PubMed1993

Natural distribution of 5 bacteria associated with periodontal disease.

The purpose of this study was to determine the prevalence and distribution of 5 bacterial pathogens in subgingival plaque, their relationship with each other and probing depth. Plaque was collected from 6905 sites in 938 subjects. A bacterial concentration fluorescence immunoassay and bacterial specific monoclonal antibodies were used to determine the presence and level of P. gingivalis (Pg), A. actinomycetemcomitans (Aa), P. intermedia (Pi), E. corrodens (Ec) and F. nucleatum (Fn) in each plaque sample. The prevalence in subjects was lowest for Pg (32%) and highest for Ec (49%). The site-based frequency distribution of these bacterial species ranged from 10.3% for Pg to 18.7% for Ec. Pi and Ec were the bacterial combination most often found together in a subject (27.2%). While 64.0% of the sites were without any of the 5 bacterial species evaluated, 20.2% had only 1 of the 5 bacterial species evaluated. The remaining 15.8% of sites had at least 2 bacteria species present. There was a general linear association of the detection level of bacterial species and probing depth. The odds ratios were 3.9 (Pg), 3.0 (Aa), 4.0 (Pi), 2.7 (Ec) and 2.8 (Fn) of finding high levels of these bacterial pathogens at > 5 mm probing depth (p < or = 0.01). Mean probing depth at molar sites without a specific bacteria was greater (p < or = 0.01) in subjects with a specific bacterium compared to molar sites in subjects without the bacteria.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗

PubMed1992

Minocycline slow-release formulation effect on subgingival bacteria.

The purpose of this study was to determine the microbiological efficacy of an adjunctive minocycline periodontal formulation delivered subgingivally. Subjects were systemically healthy but exhibited severe periodontitis; i.e., probing depths greater than 6 mm. The two study groups included individuals who received minocycline or a placebo periodontal formulation after root planing. Subgingival plaque samples were obtained at baseline; prior to treatment; and at 1, 3, and 6 months. Plaque was evaluated by darkfield microscopy and further analyzed for total dark-pigmented Bacteroides species, P. intermedia, P. gingivalis and Streptococcus, Actinomyces, Eikenella, Actinobacillus, Capnocytophaga, and Fusobacterium species using cultivable flora techniques. In addition, plaque was evaluated for yeast on a selective agar medium. When compared to the placebo, the minocycline group had significantly lower proportions of spirochetes at 1 and 3 months and lower proportions of motile rods at 3 months. Furthermore, when compared to the placebo group, the minocycline patients had lower mean proportions of dark-pigmented Bacteroides spp. and P. intermedia at 1 and 3 months as well as lower proportions of E. corrodens at 1 month. The minocycline group had significant decreases in proportions of spirochetes at 1 and 3 months, motile rods at 1 and 3 months, and increases in cocci at 1, 3, and 6 months when compared to baseline. In the placebo group, root planing was also effective at decreasing spirochetes at 1, 3, and 6 months, but with significant differences seen only at 3 and 6 months. However, the degree of reduction in spirochete proportions was greater in the minocycline group when compared with the placebo group.(ABSTRACT TRUNCATED AT 250 WORDS)

Actinomyces↗

PubMed1990

Comparison of measurement variability using a standard and constant force periodontal probe.

This study compared the intra- and inter-examiner reliability of the Florida Probe, Florida Disk Probe, and conventional periodontal probe. Using these instruments, each of three examiners made repeated measurements of probing depth (using the Florida Probe and conventional probe), relative attachment level (using the Florida Disk Probe), and clinical attachment level (using a conventional probe) at posterior, proximal sites in 10 subjects with early periodontitis. The mean intra-examiner standard deviation of differences in repeated probing depth measurements ranged from 0.46 to 0.77 mm demonstrating similar levels of reproducibility between the Florida Probe and conventional probe. The mean intra-examiner standard deviation of differences in repeated relative attachment level measurements using the Florida Disk Probe ranged from 0.44 to 0.57 mm using double passes and from 0.98 to 1.41 mm using single passes of measurements. For clinical attachment level measurements obtained using single passes of measurements with a conventional probe, the mean intra-examiner standard deviation of differences ranged from 0.78 to 0.95 mm. Inter-examiner variability was generally greater than intra-examiner variability for all instruments. It was concluded that double passes with the Florida Disk Probe offers significant advantages for measuring relative attachment level in longitudinal studies of early periodontitis when decisions for change must be made as soon as possible.

Bias↗