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Biomedical subjects

J Storek

Publications and source records attributed to J Storek.

49 records · Page 3Linked to original sources
PubMed1992

Recovery of mononuclear cell subsets after bone marrow transplantation: overabundance of CD4+CD8+ dual-positive T cells reminiscent of ontogeny.

Patients after bone marrow transplantation are immunodeficient for months to years. To understand better the pathogenesis of this immunodeficiency, we studied quantitative reconstitution of blood monocytes, natural killer (NK) cells, T cells, and B cells at 2-22 months post-transplant. The results indicate monocyte and NK counts generally recover within 2 months, followed by CD4-CD8+ T cell, B cell, and finally (after > 1 year) CD4+CD8- T cell numbers. Dual-positive CD4+CD8+ T cells (which were barely detectable in normal adults), CD4-CD8+ T cells and B cells transiently reached supranormal levels during recovery. Both CD4+CD8- and CD4-CD8+ T cells were larger than controls throughout the 2-year follow up. Comparison with neonatal and infant mononuclear cell subsets suggested the reconstitution of CD4+CD8+ T cells and B cells is similar to ontogeny. In contrast, the reconstitution of CD4+CD8- T cells did not resemble ontogeny.

B-Lymphocytes↗
PubMed1992

Reconstitution of B cell immunity following bone marrow transplantation.

Bone marrow transplant recipients are known to be immunodeficient in cellular and humoral immune responses for months to years. Mechanisms underlying the post-transplant immunodeficiency are mostly unknown and therefore it has been difficult to design therapeutic strategies to address this problem. Here, we review studies of post-transplant B cell (humoral) immunity including B cell blood counts, phenotype, function and origin, and the morphology of lymphoid organs. We postulate that the aetiology of post-transplant humoral immunodeficiency is multifactorial, involving: B cell immaturity due to recapitulation of their ontogenesis, and, in some patients, insufficient T cell help and/or exaggerated CD8+ T cell/NK cell suppression. Implications for clinical practice are outlined, e.g. hypersensitivity reactions and autoimmune diseases as part of the differential diagnosis of post-transplant disorders, questionable diagnostic benefit of serologic studies, and usefulness of prophylactic intravenous immunoglobulin.

Antibody Formation↗
PubMed1991

Blood count changes due to mononuclear cell apheresis.

Complete blood count and CFU-GM count of 41 healthy donors was determined immediately before and immediately after mononuclear cell (MNC) apheresis. Red blood cell count dropped from a mean of 5.2 to 4.9 x 10(12)/l, the difference being significant (p less than 0.001). Platelet count was reduced from a mean of 219 to 187 x 10(9)/l, the difference was once again significant (p less than 0.005). Polymorphonuclear cell count did not change significantly, while MNC count was reduced substantially (from mean 3.1 to 2.4 x 10(9)/l, p less than 0.001). The difference between pre- and postapheresis CFU-GM count (median 11.3 versus 8.8 x 10(3)/l respectively) was on the borderline of statistical significance (p = 0.05).

Adult↗
PubMed1991

Intermittent-flow versus continuous-flow mononuclear cell apheresis.

An intermittent-flow (Haemonetics V-50) and a continuous-flow (Fenwall CS-3000) blood cell separator were compared regarding mononuclear cell (MNC) collection. 17 evaluable V-50 and 24 CS-3000 aphereses were performed on healthy donors. No significant difference was found in MNC yield (median 6.4 x 10(9) on V-50, 6.3 x 10(9) on CS-3000) or in CFU-GM yield (median 41 x 10(3) versus 43 x 10(3) respectively). Nevertheless, red blood cell as well as polymorphonuclear cell contamination was substantially greater on V-50 than on CS-3000, while platelet contamination was slightly lower on V-50 than on CS-3000. The continuous-flow apheresis system CS-3000 is concluded to be more suitable for MNC harvesting than the intermittent-flow system V-50.

Adult↗
PubMed1989

[Comparison of the Haemonetics V50, Fenwal CS 3000 and IBM 2997 blood separators].

Haemonetics V 50 (HV-50), Fenwal CS 3000 (FCS-3000) and IBM 2997 separators are compared according to the results of donor plateletaphereses. The mean platelet yield of HV-50, FCS-3000 and IBM-2997 was 464 X 10(9), 551 X 10(9) and 468 X 10(9) respectively (the difference both between FCS-3000 and HV-50 and between FCS-3000 and IBM-2997 being significant - p less than 0.01). The median leukocyte contamination of platelet concentrates was 0.3 X 10(9), 0.2 X 10(9) and 5.3 X 10(9) respectively (the difference between FCS-3000 and HV-50 being not significant). The separators are further compared on the basis of platelet concentrate volume, erythrocyte contamination of thromboconcentrates, processing time, donor and staff convenience, our experience concerning product reliability and service quality, and finally the price of both the separators and the disposables.

Adult↗
PubMed1989

[Ultrasonography in emergency abdominal surgery].

Based on examinations of 416 patients with acute abdomen the authors discuss the importance of ultrasonographic examinations. In all conditions and pathological conditions with an acute abdominal symptomatology ultrasonography of the abdomen is a valuable diagnostic asset. In the complex of clinical, biochemical and X-ray examinations it hastens the diagnosis and makes it more accurate, makes an early and expedient conservative or surgical intervention possible and improves the prognosis.

Abdomen, Acute↗