PubMed Health⌕ Search

Biomedical subjects

J Sundin

Publications and source records attributed to J Sundin.

17 recordsLinked to original sources
PubMed1999

High yield direct 76Br-bromination of monoclonal antibodies using chloramine-T.

Monoclonal antibody (MAb) A33 was labeled with the positron emitter 76Br (T(1/2) = 16.2 h). Direct labeling was done using the conventional chloramine-T method. After optimization of the labeling conditions, a maximum yield (mean +/- max error) of 77 +/- 2% was obtained at pH 6.8. In vitro binding of 76Br-A33 to SW1222 colonic cancer cells showed that the immunoreactivity was retained. Also, the MAbs 38S1 and 3S193 and the peptide hEGF were 76Br-labeled, resulting in labeling yields (mean +/- max error) of 75 +/- 3%, 63 +/- 4%, and 73 +/- 0.1%, respectively. We conclude that antibodies and peptides can be labeled conveniently with 76Br for the purpose of whole-body tumour imaging by positron emission tomography.

Antibodies, Monoclonal↗
PubMed1999

Worlds we have lost and worlds we may regain: two centuries of changes in the life course in Sweden.

"The article seeks to place into historical context the familial changes in ¿post-industrial' Sweden during the past two decades, by comparing them with general characterizations (based on documented life-course experiences) of the traditional Swedish agrarian society (before 1800), the transitional society (c. 1800-1870), and the industrial society (c. 1870-1980). Familial lives in traditional Swedish society tended to be stable. By contrast, during the agrarian-to-industrial society, especially since World War II, stability became once again the hallmark because of general government social policy."

Developed Countries↗
PubMed1999

Kinetic analysis of 52Fe-labelled iron(III) hydroxide-sucrose complex following bolus administration using positron emission tomography.

Kinetic analysis of a single intravenous injection of 100 mg iron(III) hydroxide-sucrose complex (Venofer) mixed with 52Fe(III) hydroxide-sucrose as a tracer was followed for 3-6 h in four generally anaesthetized, artificially ventilated minipigs using positron emission tomography (PET). The amount of injected radioactivity ranged from 30 to 200 MBq. Blood radioactivity, measured by PET in the left ventricle of the heart, displayed a fast clearance phase followed by a slow one. In the liver and bone marrow a fast radioactivity uptake occurred during the first 30 min, followed by a slower steady increase. In the liver a slight decrease in radioactivity uptake was noted by the end of the study. A kinetic analysis using a three-compartment (namely blood pool, reversible and irreversible tissue pools) model showed a fairly high distribution volume in the liver as compared with the bone marrow. In conclusion, the pharmacokinetics of the injected complex was clearly visualized with the PET technique. The organs of particular interest, namely the heart (for blood kinetics), liver and bone marrow could all be viewed by a single setting of a PET tomograph with an axial field of view of 10 cm. The half-life (T1/2) of 52Fe (8.3 h) enables a detailed kinetic study up to 24 h. A novel method was introduced to verify the actual 52Fe contribution to the PET images by removing the interfering radioactive daughter 52mMn positron emissions. The kinetic data fitted the three-compartment model, from which rate constants could be obtained for iron transfer from the blood to a pool of iron in bone marrow or liver to which it was bound during the study period. In addition, there was a reversible tissue pool of iron, which in the liver slowly equilibrated with the blood, to give a net efflux from the liver some hours after i.v. administration. The liver uptake showed a relatively long distribution phase, whereas the injected iron was immediately incorporated into the bone marrow. Various transport mechanisms seem to be involved in the handling of the injected iron complex.

Bone Marrow↗
PubMed1999

Pharmacokinetics and red cell utilization of iron(III) hydroxide-sucrose complex in anaemic patients: a study using positron emission tomography.

The pharmacokinetics of a single intravenous injection of 100 mg iron hydroxide-sucrose complex labelled with a tracer in the form of 52Fe/59Fe was followed in six anaemic patients for a period ranging from 6 to 8 3 h using positron emission tomography (PET). Red cell utilization of the labelled iron was followed for 4 weeks. PET data showed radioactive uptake by the liver, spleen and bone marrow. The uptake by the macrophage-rich spleen demonstrated the reticuloendothelial uptake of this iron preparation, with subsequent effective release of that iron for marrow utilization. Red cell utilization, followed for 4 weeks, ranged from 59% to 97%. The bone marrow influx rate constant was independent of blood iron concentration, indicating non-saturation of the transport system in bone marrow. This implied that higher doses of the iron complex can probably be used in the same setting. A higher influx rate into the marrow compared with the liver seemed to be consistent with higher red cell utilization. This would indicate that early distribution of the injected iron complex may predict the long-term utilization.

Adult↗
PubMed1996

Minimally invasive harvesting of rectus abdominis myofascial flap in the cadaver and porcine models.

The rectus abdominis muscle has been used in reconstructive surgery as a superiorly and an inferiorly based pedicle flap as well as free flap. Since flap necrosis is unusual, the primary morbidity of the harvesting is donor-site complications, including infections, seromas, poor cosmesis, and hernias. Minimally invasive surgery has been used in abdominal, thoracic, and urologic surgeries with favorable results. To date, flap harvesting and other soft-tissue surgeries have been considered inaccessible to minimally invasive surgery based on existing techniques. We demonstrate in the (5) cadaver and (5) porcine models the technique of endoscopic harvesting of a superiorly based vertical myofascial pedicle flap. Without insufflation, we create a soft-tissue space to operate within using external skin traction. We demonstrate that this flap harvesting can be performed without the obligatory large skin incision. Donor-site complications may be decreased with less tissue disruption. In our cadaver models, we have tried using the rectus without the anterior fascia based on its superior pedicle for breast reconstruction. For this purpose, we use the endoscissors with cautery to create a tunnel up on the chest wall. The muscle based on its superior pedicle could be rotated up on the chest wall subcutaneously, allowing primary closure of the anterior sheath using the endostapler. If the anterior sheath is sacrificed or cannot be closed primarily, mesh can be used to create a reinforcing layer stapled to the edges of fascia and midline. Endoscopic surgery offers an excellent alternative for soft-tissue reconstruction without compromising the results.

Animals↗
PubMed1995

The social and cultural history of medicine and health in Sweden.

The social and cultural history of medicine and health is a growing field of research in Sweden, stimulated by the present political, economic and social concern about health and health care. Since there have never been any chairs in the history of medicine within the medical faculty, the topic has mostly been approached by historians of science and ideas, social historians and anthropologists and sociologists interested in long-term developments. Psychiatry and psychiatric care is one of the most popular subjects. Other fields are professional history and social policy, cultural and local studies on different aspects, epidemics and public health and socio-demographic studies of mortality and morbidity. This report presents some major Swedish contributions and gives a short summary of some of the results. There has, during the recent years, been a trend towards interdisciplinary exchange, for instance through Swedish and Scandinavian networks, and towards an international orientation that has led to comparative studies and exchange of results, a trend that can also be observed in many other countries.

Health Policy↗
PubMed1994

Minimally invasive harvesting of the latissimus dorsi.

The latissimus dorsi has been used extensively and dependably in reconstruction surgery. The major complications involve donor-site infections, seromas, and poor cosmesis. Minimally invasive surgery has been used in abdominal, thoracic, and urologic surgeries with favorable results. To date, flap harvesting and other soft-tissue surgeries have been considered poorly accessible to minimally invasive surgery based on existing techniques. We demonstrate in the cavader model the minimally invasive harvesting of a latissimus dorsi myofascial pedicle flap. This is performed with a laparoscope and commercially available instruments through three small incisions. External traction on the skin elevates the underlying subcutaneous tissues, creating a soft-tissue cavity without insufflation. Donor-site complications may be decreased as a result of less tissue disruption.

Endoscopy↗
PubMed1981

[Not Available].

Explore the source record for details and available documents.

Demography↗
PubMedDate not supplied

Endoscopy mastectomy and breast reconstruction: endoscopic breast surgery.

This article describes a new method of external traction elevation which is applied to the breast in order to perform an oncologically thorough glandular removal and axillary dissection followed by immediate autologous reconstruction while significantly reducing wound morbidity and greatly improving cosmesis.

Animals↗
PubMedDate not supplied

Endoscopy for regional lipectomy.

With the advances in endoscopic surgery, reconstructive surgeons search for applications in the field of soft tissue contouring. The improvements in endoscopic surgery in other fields are based on surgery within well-contained spaces that allow insufflation. However, minimally invasive surgery in the soft tissue planes has been limited by the relative inability to control separation of the tissue planes to allow a working space for instrumentation. This animal model shows the ability to open up and elevate any soft tissue space by means of external traction elevation and exact separation of tissue layers to allow endoscopic surgery without the need for insufflation. With the use of endoscopic equipment, the removal of fat from any area of the body becomes a much more precise art because of visualization and illumination and the ability to gain immediate hemostasis while exact contouring through removal of layers of fat can be achieved.

Adipose Tissue↗
PubMedDate not supplied

Effects of dextranation on the pharmacokinetics of short peptides. A PET study on mEGF.

The effects of dextranation on the biodistribution of mouse epidermal growth factor (mEGF, 6 kDa) were assessed. By reductive amination, mEGF was coupled to 13 and 46 kDa dextran. The two dextranated conjugates and free mEGF were labeled with the positron-emitting nuclide (76)Br (T(1/2) = 16 h). After intravenous administration to Sprague Dawley rats, the radioactivity biodistribution was evaluated by positron emission tomography (PET) and by measurements of dissected tissues. The dextranation prolonged the retention time in blood, especially when the dextran chain was long. [(76)Br]mEGF-dextran conjugates were shown to have significantly, more than 5 times, lower kidney accumulation than the nonconjugated [(76)Br]mEGF. In conclusion, dextranation affects the biodistribution of mEGF in vivo giving a prolonged circulation time, a decreased uptake in kidney, and an increased spleen accumulation.

Animals↗