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Biomedical subjects

J Svejcar

Publications and source records attributed to J Svejcar.

At least 19 recordsLinked to original sources
PubMed1979

Correlation of vertebral malformations with the synthesis and content of mucopolysaccharides during chondrogenesis.

Treating mice of strain C57BL/6Ffm on day 9 of gestation with 10 mg/kg of 5-fluoro-2'-deoxycytidine (FCdR) resulted in malformations of the thoracic vertebral column (ThVC) in 98% of near-term fetuses (Degenhardt et al., 1968). The spectrum of malformations was broad: fusion, dysplasia, cleft, aplasia and hypoplasia were all produced. Fusions of two or more segments represented more than half of all malformations (Bosse, 1978). The alterations in embryonic precartilage and cartilage after FCdR-treatment were followed from day 11 to day 15 in a biochemical and histological study. Biochemically, the 35S-uptake into embryonic mucopolysaccharides (MPS) and the content of total MPS and seven fractions of MPS in embryos or isolated ThVCs were analyzed. The histological variables studied were the types and incidence of malformations of the ThVC, 35S-autoradiography of the ThVC, and the amount of alcian blue-stained cartilaginous matrix. The results showed that on day 11 the synthesis of embryonic MPS was not affected, on day 12 the synthesis of MPS was greatly reduced, on day 13 the synthesis of MPS was slightly reduced while the MPS-content was not affected. On day 13 aplasias were seen in the same percentage as at term, but no fusions were detected. By day 14 the MPS-content was greatly reduced; hyaluronate, condroitin 4-sulfate, and chondroitin 6-sulfate being principally involved; the first fusions were seen. On day 15 the MPS-content was slightly reduced (chondroitin 6-sulfate and heparan sulfate were involved), fusions were complete. The results are discussed in terms of disturbance of structure and function of the notochord and intervertebral discs with the production of fusions, the main type of vertebral malformation in these experiments.

Animals↗
PubMed1979

The effect of native and sonicated double-stranded polyribonucleotides on the course of spontaneous autoimmune disease in NZB and NZB/Swiss F1 mice.

The administration of sonicated fractions of f2 phage polyribonucleotides caused an increased weight loss and deterioration of the clinical state in female NZB mice. Discontinuance of the treatment resulted in an improvement of both the clinical state and the genetically determined autoimmune disorders of these mice. Some potential explanations of this effect are discussed.

Animals↗
PubMed1977

[Methodical approaches to the promotion and maintenance of breast-feeding (author's transl)].

In the last years work done in the field of child nutrition has shown the great advantage of breast-feeding as to artificial feeding. Therefore it is necessary to seek new ways to promote this type of feeding which is continuously falling. It could be shown that by an intensive health education in the public, in the obstetric clinics, and in the family, the disposition of the mothers to breast-feed can be raised even nowadays by convincing them of its value. This aim will be best supported by intensive teaching in the neonatal clinics of breast-feeding and of application of the system "ad libitum", in the clinic and even more after returning home. At home this system of nutrition is important for the maintenance of breastfeeding in the first weeks and even longer.

Breast Feeding↗
PubMed1976

Influence of thymosterin on immunological reactivity in New Zealand mice.

The influence of thymosterin on immunological reactivity of NZB mice was investigated. Thymosterin was administered three times per week for a period of six weeks to male and female NZB mice. Only lymphocytes from untreated female mice gave the positive results in the macrophage migration inhibition test using native DNA as the stimulating antigen. This effect was suppressed in thymosterin treated females. The administration of thymosterin therefore appears to correct the in vitro delayed hypersensitivity to DNA antigen.

Animals↗
PubMed1976

Effect of immunosuppressive drugs on an in vito correlate of cell-mediated immunity. The migration inhibition test.

The effect of a number of immunosuppressive drugs on the migration-inhibition test was studied. These drugs could be divided into four categories: (1) drugs influencing both the MIF production and MIF effect on macrophages (amethopterin, azathioprine); (2) drugs influencing MIF production (puromycin, actinomycin D); (3) drugs influencing the effect of MIF on macrophages (hydrocortisone); (4) drugs without any influence.

Animals↗