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J Szarapińska-Kwaszewska

Publications and source records attributed to J Szarapińska-Kwaszewska.

At least 19 recordsLinked to original sources
PubMed2001

[Utilization of exogenous siderophores by enterococci].

The study was undertaken to investigate the ability of enterococci to assimilate iron via siderophores of bacteria living in the same habitats in the human organism. The potential recipients of exogenous siderophores were six Enterococcus faecalis and six Enterococcus faecium strains, isolated from clinical materials of human origin. The donors of siderophores were Gram-negative rods (various species of the Enterobacteriaceae, Pseudomonas and Acinetobacter) and Gram-positive cocci (various species of Staphylococcus and Streptococcus). All of the investigated E. faecium and only two E. faecalis strains demonstrated the ability to utilize the siderophores of the aforementioned bacterial groups, predominantly the chelators of Gram-negative rods, those of Gram-positive cocci were utilized to a smaller extent. Four recipient strains from E. faecalis species did not demonstrate the ability to utilize siderophores synthesized by all of 40 investigated donor strains.

Enterococcus↗
PubMed2001

[Alive microorganism in the workplace ambient air in plants disposing communal waste].

This paper presents the results of the identification and determination of alive microorganisms in the air samples collected from the breath zone of workers employed in the waste collection and disposal. Samples were taken during waste collection and in the waste reloading, sorting and composting plants. In addition, samples were taken at stationary sites: in the city and inside flats. Different kinds of species of Gram-positive bacteria (Micrococcus, Staphylococcus, Enterococcus, Bacillus, Listeria, Corynebacterium, Rhodococcus) and Gram-negative rods (Enterobacteriaceae, Pseudomonas and other aerobic bacteria) were found. On every work site, there were fecal bacteria (Escherichia coli, Enterococcus faecalis and Enterococcus faecium). The city areas and flats were free from this kind of bacteria. The number of mesophilic bacteria and thread-like fungi exceeding 10(4) cfu/m3 of air was counted on every work site. The largest number of mesophilic bacteria and Actinomycetes were found in the waste composting plant (10(4)-10(6) and 10(3)-10(5) cfu/m3, respectively), whereas the majority of fungal spores were counted during waste collection and sorting. (10(4)-10(5) cfu/m3). On the city streets there were no Actinomycetes and the number of fungal spores (10(4)/m3 of air) was similar to that observed in the waste reloading and composting plants. The amount of mesophilic bacteria in the city and flat samples was below 10(2)-10(3) cfu/m3. As to the number of fungal spores and Actinomycetes there was a limit of determination (10(1)-10(2) cfu/m3). The majority of mesopholic bacteria identified in the air were classified as the second group of occupational risk in the Council Directive 54/2000/EU. Bearing in mind that Gram-negative rods (especially intestinal) were identified in all samples, it is suggested to use endotoxin concentration as one of possible criteria for assessing the hygiene conditions at workplaces.

Air Microbiology↗
PubMed2001

[Assessment of exposure to bioaerosols in workplace ambient air during municipal waste collection and disposal].

The assessment of the exposure to bioaerosols among workers engaged in the collection and disposal of municipal waste is presented. The workers were divided into the following groups, depending on the job performed: waste collectors (loaders, drivers), composting plant workers (heavy equipment operators, waste site workers), sorters (heavy equipment operators, waste sorters) and landfill site workers (heavy equipment operators, weighers). Air samples were also collected on the city streets and in flats. They were reference points to the results obtained. Air samples were collected at the breathing zone. The concentrations of dust, viable microorganisms (mesophilic and thermophilic bacteria, and fungi), endotoxins and the total number of microbiol cells were determined. The highest, individual exposure to dust was found in composting plant workers and waste collectors. The values exceeded considerably maximum allowable concentrations (by ten times at composting plant). The mean values for the above listed groups also exceeded the concentration of 4 mg/m3. Dust concentrations at other workposts were substantially lower. The concentrations in the city streets and in flats maintained at the level of 0.1 mg/m3. Endotoxin concentration at a protecting upper airway inflammation level (10 ng/m3) was exceeded in the majority (above 50%) of samples. That is why the average concentration in individual groups was also higher than 10 ng/m3. Street and flat samples included endotoxins at the level of 1 ng/m3. Taking as a criterion the obligatory total dust and endotoxin MAC value of 10 ng/m3 for assessing work hygiene conditions, it should be stated that waste collectors and composting plant workers were employed in conditions of poor hygiene.

Adult↗
PubMed2000

[The interchangeability of siderophores in the Enterococcus genus].

The functional interchangeability of siderophores was tested among 62 strais belonging to 12 species of genus Enterococcus. Most investigated strains were from E. faecalis and E. faecium species. The majority of examined enterococcal strains appeared highly resistant to EDDA (ethylene-di-amine-di-ortho-hydroxyphenylacetic acid), therefore the group of sensitive strains involved only 11 used as indicator strains. The determination of interchangeability of siderophores within enterococcal strains was performed using EDDA-agar media into which the indicator strains were included. Test colonies (donor strains) were applied to the surface of the media to determine whether the indicator organisms could obtain the required iron for growth by utilizing chelators from the test colony. Only two strains: E. solitarius DSM 5634 and E. pseudoavium DSM 5632 did not demonstrate the ability to utilize siderophores synthesized by all investigated strains. The other tested indicator strains appeared to be recipients of siderophores from 20-52 donor enterococcal strains. The ability to exchange siderophores in enterococci was found as the feature characterizing individual strains.

Enterococcus↗
PubMed1999

[Studies on siderophore exchange properties between staphylococci and various species of gram-positive and gram-negative bacteria].

The ability of iron utilizing by means of staphylococcal siderophores by bacteria belonging to genera: Acinetobacter, Corynebacterium, Curtobacterium, Clavibacter, Bacillus and Mycobacterium was investigated. The staphylococcal donor strains (18 species) used in these experiments were characterized by the ability to utilize siderophores produced by various strains belonging to aforenamed genera. The utilization of staphylococcal siderophores was studied on agar media in which minimally effective concentrations of ethylenediaminedi-ortho-hydroxyphenylacetic acid (EDDA) were used to inhibit indicator strains. Test colonies (staphylococcal) were applied to the surface of the media to determine whether the indicator organisms could obtain the required iron for growth by utilizing chelators from the test colony. The growth inhibition by EDDA of most strains from the Acinetobacter rods and from the coryneform-organisms (plant pathogen) genera, and strains from the species: B. subtilis, M. phlei, M. smegmatis, M. fortuitum was reversed by staphylococcal siderophores. None of the staphylococcal strains investigated, had the ability to exchange siderophores with strains from the species: C. pseudodiphtheriticum, Corynebacterium ANF group, B. megaterium, M. vaccae, M. chitae and M. parafortuitum.

Edetic Acid↗
PubMed1998

[Utilization of siderophores from mycobacteria by Staphylococcus].

The ability of iron utilizing by means of siderophores (extracellular exochelins and cell-associated mycobactins) produced by mycobacteria (7 stains) by 24 staphylococcal strains was investigated. The mycobacterial donor strains belonged to rapid growing species: M. fortuitum, M. smegmatis, M. aurum, M. vaccae, M. chitae, M. phlei, M. parafortuitum. The utilization of mycobacterial siderophores was studied on agar or liquid media in which minimally effective concentrations of ethylene diamine di-ortho-hydroxyphenyl acetic acid (EDDA) were used to inhibit indicator staphylococcal strains. Mycobacterial siderophores (exochelins or mycobactins) reversed the inhibition of the majority (22/24) staphylococcal strains. Most of strains utilized exochelins from M. phlei as well as mycobactins from M. parafortuitum and M. chitae.

Iron Chelating Agents↗
PubMed1998

[Utilization of siderophores from the Acinetobacter genus by staphylococcal bacilli].

The ability of iron utilizing by means of siderophores produced by donor strains--the members of the genus Acinetobacter (8 strains) by 24 staphylococcal strains was investigated. All the donor strains synthesized hydroxamate class siderophores and six strains also catecholate class. The majority of staphylococcal strains could utilize these siderophores. Most strains utilized siderophores from A. juni 321 and A. johnsonii 349 strains. Only three staphylococcal strains were not be able to utilize siderophores from all donor strains.

Acinetobacter↗
PubMed1997

[Utilization of Staphylococcus siderophores produced by Corynebacterium and Coryneform bacteria].

The ability of iron utilizing by means of siderophores produced by donor strains-Coryne-bacterium and coryneform organisms (8 strains) by 24 staphylococcal strains was investigated. All the donor strains synthesized catecholate class siderophores and two strains also hydroxamate class. The majority of staphylococcal strain could utilize these siderophores. Most of strains utilized siderophores from Corynebacterium pseudodiphtheriticum and Corynebacterium aquaticum as well as chelators from plant pathogens-coryneform organisms. Only two staphylococcal strains were not be able to utilize siderophores from all donor strains.

Actinomycetales↗
PubMed1996

[Staphylococcal utilization of siderophores produced by bacilli of the genus Bacillus].

The ability of utilization by 24 staphylococcal strains of the siderophores of B. megaterium PCM 2010 and B. subtilis S was investigated. B. megaterium PCM 2010 produced hydroxamate class siderophores and B. subtilis S-catecholate class. The majority of staphylococcal strains--thirteen--could utilize these siderophores: eight strains from both these species and three strains did not possess any receptors for these chelators. The staphylococcal strains producing catecholate siderophores utilized catecholate chelators from B. subtilis S.

Bacillus↗
PubMed1994

[Oxygenation of pesticides by pseudomonas aeruginosa as a way for creation of mutagenic compounds].

During biodegradation of pesticides there is a possibility of appearance of toxic metabolites. In this aspect oxygenation of 15 new chemical compounds possessing pesticide activity by Pseudomonas aeruginosa PS6749 was investigated. By application of the Ames test, mutagenicity of appearing products of reaction was investigated. Utilization of oxygen in presence of 11 compounds was found. Metabolites of pesticide IPO 3819-N/3,4-dimethoxybenzylidene/2-hydroxyaniline were mutagenic for a strain of Salmonella typhimurium TA 98. Oxygenation products of remaining compounds did not exhibit mutagenic activity.

Biodegradation, Environmental↗
PubMed1992

[The role of bacterial metabolism in transformation of non-mutagenic compounds into mutagens. I. Participation of Escherichia coli nitroreductase in creation of mutagens from non-mutagenic new pesticides].

Investigations concerned Escherichia coli nitroreductase in creation of mutagens from non-mutagenic pesticides-derivatives of urea. Three new compounds were studied: N-phenyl-N'-methylurea (IPO 4328), N-methyl,N-(2-hydroxyethyl)-N'phenylurea (IPO 2363), N-(2-hydroxyethyl), N-methyl-N'-(3,4 dichloroethyl) urea, and diurone-3-(3,4 dichlorophenyl)-1,1 dimethylurea. These compounds were incubated in anaerobic conditions with cells of E. coli K-12 (KF) strain and nitrate or nitrite. Using Ames test, mutagenicity of resulting metabolites was investigated. It was found that during incubation of herbicide IPO 4328 with cells of E. coli K-12 (KF) and nitrate, mutagenic product for strain of S. typhimurium TA 1537 is created. Very weak mutagenic metabolite for the same strain was appearing during incubation of herbicide IPO 2363 with cells of E. coli K-12 (KF) in presence of nitrite. Incubation of investigated compounds with E. coli K-12 (KF) cells alone did not result in appearance of mutagenic substances. Thus, role of Escherichia coli in creation of mutagenic compounds from non-mutagenic derivatives of urea consisted of nitrite from nitrate production with participation of nitroreductase, which afterwards in absence of bacteria or action of their enzymes reacted with investigated pesticides.

Escherichia coli↗
PubMed1992

[Role of bacterial metabolism in transformation of non-mutagenic compounds into mutagens. II. Participation of bacteria producing urease in degradation of pesticides--urea derivatives].

Participation of bacteria producing urease: Proteus mirabilis and Staphylococcus epidermidis in degradation of pesticides--urea derivatives, was investigated. Four new compounds were studies: N-/(3-trifluoromethyl)phenyl/N'-(2-cyanoethyl)-urea (IPO 6584), N-(4-chlorophenyl)-N'-(2-hydroxyethyl)-N'-ethylurea (PO 6236), N-(4-chlorophenyl)-N'-(2-hydroxyethyl), N'-propyl-urea (IPO 6237), N-(2-hydroxyethyl), N-methyl-N'-(3,4 dichlorophenyl)-urea (IPO 3102), pesticide reference standard--thiram fungicide--tetramethyl-bis-thiocarbamyl disulfide, and rodenticide-alpha-naphthylthiourea (ANTU). Investigated compounds were incubated with cells of P. mirabilis 4508 ans S. epidermidis CCM 2448 and commercial preparation of urease from beans. Mutagenicity of resulting metabolites was then studies by the Ames test. All compounds were decomposed by bacteria used in this study, as well with beans urease with different activity. Reaction products did not exhibit mutagenic activity for test strains S. typhimurium his- TA97a, TA98, TA100 and TA102.

Biotransformation↗
PubMed1978

Coagulase in starved Staphylococcus aureus.

The resting cells of S. aureus strain Smith (diffuse) lost marked amount of free amino acids and proteins during their starvation. The starved cells contained less coagulase and that enzyme was released into the medium during starvation. After transfer into nutrient medium, those cells produced less coagulase than the non-starved ones. The restoration of amino acid pool resulted in coagulase production on initial level.

Amino Acids↗
PubMed1977

Factors influencing L-asparaginase production by staphylococci.

Cultural and nutritional requirements for a maximum synthesis of 1-asparaginase by staphylococci were determined. The best production of the enzyme was found in the stationary phase of growth of a batch culture. The highest 1-asparaginase yield was obtained when the culture were aerated during an exponential phase of growth and further incubated in the stationary phase. Optimum pH for the enzyme production was 7.5. Glucose inhibited the enzyme formation. Maximum yield of 1-asparaginase was obtained when casein hydrolysate and yeast extract were supplied as carbon and nitrogen sources. Repression by 1-asparagine and 1-aspartic acid was absent.

Air↗