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Biomedical subjects

J Sznajd

Publications and source records attributed to J Sznajd.

At least 73 records · Page 4Linked to original sources
PubMed1982

Ribonuclease deficiency in lymphocytes of patients with chronic lymphocytic leukemia (CLL).

Ribonuclease (RNase) activity in the lymphocytes of 20 chronic lymphocytic leukemia (CLL) patients and 10 normal subjects was studied. It was found that in the lymphocytes of the control subjects the RNase activity could be detected in the pH range 4.5 to 8.6, inclusive. The RNase activity versus pH profile of normal lymphocytes consists of an acid RNase peak at pH 6.5 and alkaline RNase peak at pH 7.8. When treated with pCMB an inhibitor-bound RNase activity was revealed. The peak of this activity lay between pH 6.7 to 7.0. Liberating the inhibitor-bound RNase activity changed the RNase activity-pH profile, yielding one peak curve with a maximum at pH 7.0. RNase activity in CLL lymphocytes was remarkably lower than that in normal lymphocytes. The acid RNase in 80% of the CLL patients was lower by a factor of ten. Likewise, a many fold decrease in alkaline RNase activity (in some cases down to the zero level) was observed in CLL lymphocytes. However, in 70% of CLL patients, a level of the inhibitor-bound RNase activity was similar to that found in normal lymphocytes. In 20% of the studied CLL patients, a remarkable decrease in both free alkaline and inhibitor-bound RNase activity was observed. When poly-C was used as a substrate for determining RNase activity, a decrease to approximately 15% in CLL lymphocytes was observed, when poly-U was used instead of poly-C, a decrease to 65% was found only as compared with normal lymphocytes. This may suggest that CLL lymphocytes are deficient in a poly-C specific RNase which displays its activity within a neutral and alkaline pH range.

Densitometry↗
PubMed1981

Serum ribonuclease activity in acute myocardial infarction.

Ribonuclease (RNase) activity measured at pH 7.8 (the alkaline RNase) and pH 6.7 (the acid RNase) was estimated in serum of 54 patients with acute myocardial infarction (AMI). The estimations were performed on the first day of disease, then on the seven consecutive days and on the 14th day. A significant elevation in both alkaline and acid RNase activities on days 1--7 of AMI was found, compared with healthy control subjects. Elevation in both acid and alkaline RNase activity began on the 1st day of AMI and continued to increase up to peak values on the 3rd day for acid RNase and the 5 the day for alkaline RNase. On the 14th day both alkaline and acid RNase activities came back to the range found in normal sera. Increase in serum acid and alkaline RNase activity in AMI showed a time pattern different from that of GOT and GPT aminotransferases. However, the relationship between the elevation of serum RNase activity and severity of the disease was obvious.

Adult↗
PubMed1979

Elevation of an acid ribonuclease in serum of patients with chronic granulocytic leukaemia.

Ribonuclease (Ribonucleate nucleotide 2'-transferase E.C. 2.7.7.17) activity in serum of patients with chronic granulocytic leukaemia measured at pH 4.5-6.0 amounts to more than three times of that in serum of healthy subjects. At pH 6.0-8.0 the elevation of ribonuclease activity in serum of patients with chronic granulocytic leukaemia is less pronounced and amounts to about two times of that in normal ones. Using chromatography on CM Sephadex C-50 column, serum ribonuclease of both normal and chronic granulocytic leukaemia patients was separated into five distinct fractions. In serum of healthy subjects ribonuclease fractions denoted I-V contribute to 10; 21; 29; 22, and 18 percent of the total ribonuclease activity. In the serum of patients with chronic granulocytic leukaemia a decrease in ribonuclease fraction III to merely 17 percent and an increase in contribution of fraction IV to 32 percent of total ribonuclease activity could be observed. The comparison of each individual concentration of fraction in normal and leukaemia patients serum reveals, that ribonuclease fraction IV will increase about 3 times. A less pronounced increase could also be found for fractions I, II and V. However, ribonuclease fraction IV may be supposed to carry more than 50 percent of the whole extra load of ribonuclease present in the serum of chronic granulocytic leukaemia patients.

Acids↗