Biomedical subjects

J T Den Dunnen

Publications and source records attributed to J T Den Dunnen.

21 records · Page 2Linked to original sources

PubMed1987

Linkage between the beta B2 and beta B3 crystallin genes in man and rat: a remnant of an ancient beta-crystallin gene cluster.

Human and rat genomic clones containing beta B2- and/or beta B3-crystallin sequences have been isolated and characterized. Both in the human and the rat genome the single-copy beta B3-crystallin gene is linked to a beta B2-crystallin gene. In both species the linked genes, separated by 20 kb in the human and 11 kb in the rat genome, are oriented head-to-tail with respect to transcription. A single copy of the beta B2-crystallin gene is present in the rat genome, in the human genome two copies of this gene are found. The second human copy could as yet not be linked to the beta B2/beta B3-crystallin gene cluster.

Animals↗

PubMed1987

All six rat gamma-crystallin genes are located on chromosome 9.

The rat genome contains six genes for the lens-specific gamma-crystallins. Five of these genes are clustered; the location of the sixth gene is not known. To determine whether the sixth gene is located on the same chromosome as the gamma-crystallin gene cluster, a panel of mouse-rat hybrid cell lines, segregating rat chromosomes, was screened for the presence of gamma-crystallin sequences. We show here that the hybrid cell lines contain either all rat gamma-crystallin genes or none, indicating that all six genes map to the same chromosome. The presence of rat gamma-crystallin sequences in the hybrid cell lines was concordant only with the presence of rat chromosome 9, which therefore contains the rat gamma-crystallin genes.

Animals↗

PubMedDate not supplied

Methods for pulsed-field gel electrophoresis.

The term pulsed-field gel electrophoresis (PFGE) is used as an acronym to indicate any technique that resolves (large) DNA molecules by continuous reorientation. It bridges the resolution gap between cytogenetic methods (> 5 Mb) and DNA analysis (< 50 kb). Initially, PFGE was used to study the chromosomal content of unicellular eukaryotic organisms of interest to genetic research and population health. Later, PFGE was used to construct megabase maps of segments of the human genome. Successfull utilization of PFGE requires the availability of very high-molecular weight DNA. This article describes the modification of standard DNA protocols necessary to handle large DNA molecules, based on its encapsidation in agarose.

DNA↗