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Biomedical subjects

J T Guthrie

Publications and source records attributed to J T Guthrie.

13 recordsLinked to original sources
PubMed1986

Investigation into the use of graft copolymer-based cibacron blue F3GA columns for the purification of proteins.

The graft copolymers Nylon-co-hydroxyethylmethacrylate and poly(ethylene)-co-hydroxyethylmethacrylate coupled to Cibacron blue F3GA at wet volume levels similar to those obtained with Sepharose 4B. However, the graft copolymers removed protein from human serum to a far lesser extent than did Sepharose 4B. Further investigations involved the preparation of hydrolyzed poly(vinyl acetate) copolymers of nylon and polyethylene and of cellulose-co-hydroxyethylmethacrylate and study of the ability of the copolymers to remove human serum albumin and lactic dehydrogenase. Comparisons were made with Sepharose 4B-, Sephadex G15-, and G25-based Cibacron blue F3GA systems. The effectiveness of Sepharose 4B-Cibacron blue F3GA is thought to be due to the manner in which the dye is located within the pores of the gel.

Adsorption↗
PubMed1980

Immobilization of beta-galactosidase and other enzymes onto p-amino-carbanilated cellulose derivatives.

beta-Galactosidase and other enzymes were immobilized on p-amino-carbanilated derivatives of cellulose and methylol cellulose using the diazo method and through glutaraldehyde. The optimum conditions for coupling cellulose tri-(p-amino-carbanilate) (CTAC) to beta-galactosidase were established. The diazo coupling method with CTAC gave greater activity than with glutaraldehyde when coupled to beta-galactosidase (Escherichia coli). The stability of the CTAC-beta--galactosidase system was examined. The disubstituted p-amino-carbanilate derivative (CDAC) gave a lower activity, whereas the methylol analog (MCTAC) gave slightly greater activity. The CTAC was also used to immobilize glucose oxidase, trypsin, pepsin, and papain.

Amines↗
PubMed1978

Reading ability and efficiency of graphemic-phonemic encoding.

It was hypothesized that children with a specific reading disability differ from children of normal reading ability because the former are impaired in extracting speech-like representations from graphemes. Three groups of 12 each of grade school boys and girls participated in timed word comparison tasks which required a "same-different" response. One group was specifically reading deficient, the other groups were matched either on age or on reading level to a deficient group. Monosyllabic word pairs were presented simultaneously by a slide projector. The deficient readers were slower than the two control groups only in a vowel phoneme comparison task and produced response times similar to the control groups for graphemic and visual symbol comparisons. The results are consistent with the hypothesis that the problem in learning to read is due to multiple relationships between spelling (graphemes) and sound (phonemes).

Child↗
PubMed1976

The genetics of specific reading disability.

Members of the immediate families of twenty children with specific reading disability were examined to determine the prevalence of reading disability within the families. A procedure was developed for identifying adults who may have compensated for a disability manifested more clearly in childhood. Forty-five percent of 75 first-degree relatives of the parents were affected and there was a significantly greater number of affected male relatives than females. No single mode of genetic transmission is evident after inspection of the pedigrees. It is suggested that the disorder is genetically heterogeneous and that subgroups of disabled readers should be looked for.

Adult↗