Biomedical subjects

J T Siebinga

Publications and source records attributed to J T Siebinga.

4 recordsLinked to original sources

PubMed2000

[Tick-borne fever (Ehrlichia phagocytophila infection) on a dairy farm in Friesland].

Disease outbreaks, associated with ixodid ticks, among cows on a dairy farm in Friesland, in the north of the Netherlands, were monitored during the summers of 1996, 1997, and 1998. The most important symptoms were a sudden drop in milk yield, fever, apathy, and problems with locomotion. The diagnosis 'tick-borne fever' was confirmed by finding Ehrlichia phagocytophila inclusion bodies in neutrophils of blood smears from sick animals. Significant economic losses due to the disease were recorded each year. Borrelia burgdorferi, although transmitted by the same tick, did not seem to be involved. Further research on tick-borne fever is of particular relevance in connection with recent E. phagocytophila cases diagnosed in dogs and in a human patient in the Netherlands.

Animals↗

PubMed1992

[Classical fowl plague and milder influenza infections in birds and mammals].

Wild waterfowl are currently considered the largest reservoir of the various haemagglutinin (H) and neuraminidase (N) subtypes of influenza virus. Until now thirteen different H-types and nine different N-types have been detected in these populations. In the first instance, virus transmission from fowl to other animal species and to man is not causing disease problems. However, small changes at the molecular level of a given HN-subtype recently caused a dramatic increase in virulence for chickens. Genes fragments coding for haemagglutinin or neuraminidase can be exchanged between viruses which propagate in the same individual. This phenomenon-'genetic reassortment'-is of major epidemiological significance when it occurs in pigs. New influenza epidemics in the human population consistently originate in areas where waterfowl, pigs and human beings live close together. At the moment, the virological and serological diagnosis of influenza A infections is based ELISAs for antigen and antibody detection. Both ELISAs employ a monoclonal antibody directed against a conserved antigenic determinant of the influenza A nucleoprotein. The use of these tests can simplify the diagnosis of and screening for influenza A infections, particularly in those species which harbour several H- and N-subtypes.

Animals↗

PubMed1988

Influenza A viral nucleoprotein detection in isolates from human and various animal species.

A double antibody sandwich, enzyme-linked immunosorbent assay (DAS-ELISA) was developed to detect influenza A viral antigen, employing a monoclonal antibody directed against type-specific influenza A nucleoprotein (McAb anti-NP). McAb anti-NP was used to coat ELISA plates as well as to prepare the peroxidase conjugate. Influenza A viruses of avian, equine, swine, and human origin were detected in allantoic fluids of inoculated eggs with higher sensitivity by the DAS-ELISA than by hemagglutination (HA) assays. Minimal concentrations of 8 ng/ml influenza virus protein were detected in Nonidet P40-treated virus preparations. Viral antigen detection in tissues of experimentally infected chickens and pigs was successful, but in pigs yielded a lower positive score than the conventional method of virus isolation in eggs. The test is sensitive, rapid, and easy to perform, but does not permit influenza A subtyping. In avian species, the McAb anti-NP DAS-ELISA differentiates between influenza and Newcastle disease viruses. In pigs, the test distinguishes between influenza and Aujeszky's disease.

Allantois↗

PubMed1985

[Winter diarrhea].

Winter dysentery is a highly contagious, herd-enzootic disease of cattle, most frequently observed during the winter season. The disease is characterized by a sudden onset of severe diarrhoea, which normally disappears spontaneously within two or three days. The aetiology continues to be obscure, though a virus is a probable causative agent.

Animals↗