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J T Sinski

Publications and source records attributed to J T Sinski.

8 recordsLinked to original sources
PubMed1991

A survey of dermatophytes from human patients in the United States from 1985 to 1987.

A survey of dermatophytes isolated from patients seeking medical advice was made from 1985 to 1987 in the United States. The survey included 54 locations with data from 45 cities and one state. Listing of the isolated dermatophytes and the frequency given by percentage of the total follows: Trichophyton rubrum 54.8%, T. tonsurans 31.3%, T. mentagrophytes 6.0%, Microsporum canis 4.0%, Epidermophyton floccosum 2.0%, M. gypseum 0.6%, and T. verrucosum 0.2%. Out of a total of 14,696 isolates M. audouinii was cultured 13 times, T. violaceum 12 times, M. nanum 6 times, T. terrestre 4 times, and T. soudanense twice. Single isolations were made of M. fulvum, M. ferrugineum and T. schoenleinii. Collection of dermatophyte data in Tucson, Arizona, began in 1966. In 1987, the first case of tinea capitis caused by T. tonsurans was observed. Other isolates of this organism as the cause of tinea capitis were made in this city during that year. These infections were in black children. With the recent growth of Tucson, the percentage of blacks in the population increased and this pathogen was introduced into the general population.

Arthrodermataceae↗
PubMed1987

A survey of dermatophytes isolated from human patients in the United States from 1982 to 1984.

A survey of dermatophytes isolated from patients seeking medical advice was made from 1982 to 1984 in the United States. The survey included 59 locations with data from 49 cities and one state. Listing of the isolated dermatophytes and the frequency given by percentage of total follows: Trichophyton rubrum 46.8%, T. tonsurans 33.3%, T. mentagrophytes 10.1%, Microsporum canis 4.5%, Epidermophyton floccosum 3,5%, M. gypseum and T. verrucosum both 0.7%, M. audouinii and T. terrestre both 0.1%, and T. violaceum 0.06%. No isolations of M. ferrugineum or T. schoenleinii were reported. Temporal increases were observed for frequencies of T. mentagrophytes, T. tonsurans and M. canis, and decreases occurred for frequencies of E. floccosum and T. rubrum when the data from this survey were compared by the goodness of fit test to data of the 1979 to 1981 survey. The percent of dermatophyte isolations identified as T. tonsurans correlated significantly with the percentage of blacks in cities of 100,000 or more people.

Arthrodermataceae↗
PubMed1979

Effect of storage temperature on viability of Trichophyton mentagrophytes in infected guinea pig skin scales.

The effect of storage temperature on the viability of Trichophyton mentagrophytes in infected guinea pig skin scales was quantitatively evaluated for 182 days. Infected skin scales were stored, while protected from light, in glass containers with desiccant at four temperatures and sampled after saline treatment at various time intervals. No decrease in viability was observed for storage at 4 degrees C, room temperature, or 30 degrees C. Storage at 37 degrees C resulted in a significant loss in viability.

Animals↗
PubMed1977

Dermatophyte isolation media: quantitative appraisal using skin scales infected with Trichophyton mentagrophytes and Trichophyton rubrum.

Various dermatophyte isolation media were quantitatively appraised by colony counts for their ability to support growth when suspensions of trypsin-treated infected-skin scales were used as inocula. Evaluation included guinea pig skin scales infected with a granular Trichophyton mentagrophyton mentagrophytes, and one trial was conducted using human skin scales infected with Trichophyton rubrum. No differences in the ability to support growth were observed among Taplin Dermatophyte Test medium, Dermatophyte Test Medium (Schering Diagnostics, Port Reading, N.J.),Sabouraud medium plus cycloheximide and chloramphenicol, Youssef medium for dermatophytes product no. 103S semisynthetic lots no. 106 and no. 107. With skin scales not grossly contaminated with bacteria, similar counts were obtained with Littman oxgall agar without antibiotics. Youssef medium for dermatophytes product no. 103N natural lot no. 109 was significantly different from the other media in that lower counts were obtained.

Animals↗
PubMed1975

Coccidioidin and merthiolate in previously sensitized animals.

The effect of merthiolate, which is used as a preservative in skin test materials, on skin test reactions was determined in guinea pigs. In four groups of animals, merthiolate in basal medium produced skin tests at 24 and 48 h characterized by erythema and/or induration in an intermediate region, i.e., 5 plus or minus 2.2 mm. One of the four groups of animals was a nonsensitized control group. The other three groups were subcutaneously sensitized with (i) merthiolate and saline, (ii) killed Coccidioides immitis arthrospores, and (iii) merthiolate with killed C. immitis arthrospores. Coccidioidin only and merthiolate in coccidioidin produced positive delayed results in groups 3 and 4, which were sensitized with arthrospores. A synergistic effect of merthiolate and coccidioidin was observed in animals of group 4 sensitized by merthiolate with killed C. immitis arthrospores. This effect was observed at 24 h when positive reactions of coccidioidin with merthiolate were significantly greater than skin tests with plain coccidioidin.

Animals↗
PubMed1975

Quantitative assay of dermatophyte-infected guinea pig skin scales.

A technique to obtain homogeneous suspensions of fungal particles in scrapings from infected skin was devised that permits delivering uniform inocula to culture media being evaluated for suitability as primary isolation media for dermatophytes. The technique consisted of trypsinization of the scales, utilizing scrapings from lesions experimentally induced with an isolate of Trichophyton mentagrophytes var. granulare on guinea pigs. Optimal conditions for dislodging the fungal particles were assessed by colony counts. One-hour treatments with 0.5% (1:300) trypsin yielded the highest counts.

Animals↗
PubMed1975

Pagano-Levin Candida test medium: evaluation using vaginal samples.

Since 1958 pagano-Levin medium (PLM) has been used as an aid in the identification of Candida albicans. However, no statistical analysis of its effectiveness based on large number of random human specimens has been reported. The present study compared PLM (now called Candida test) with Sabouraud's plus antimicrobials and Littman's ox-gall agar without antimicrobials. Of 500 random vaginal samples 24.8 were true positives, 71.9% were true negatives, 2.2% were false positives, and 1.2% were false negatives on PLM. If only samples identified as C. albicans were considered, 95.4% were true positives and 4.6% were false negatives. PLM did not inhibit C. albicans from the vagina. No one medium was found superior to the others for the purpose of isolating and identifying C. albicans. Of the five strains of C. parapsilosis, the only other Candida species isolated, all the samples grew on PLM but most were inhibited on Sabouraud's medium plus antimicrobials.

Candida↗