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J T Staley

Publications and source records attributed to J T Staley.

44 records · Page 3Linked to original sources
PubMed1971

Incidence of prosthecate bacteria in a polluted stream.

Water samples were collected aseptically several times throughout the year at nine stations on the Red Cedar River, a stream flowing through farmland and receiving effluent from several municipalities in central Michigan. Total prosthecate bacteria were enumerated by both direct and viable counting techniques. By direct techniques, these bacteria accounted for 0.62 to 1.1% of the total microflora during the study. The predominant type of appendaged bacteria was the caulobacters (Caulobacter, Asticcacaulis, and the fusiform caulobacter), which accounted for 64 to 93% of the total prosthecate forms. The others of importance were prosthecomicrobia (< 1 to 24%), including Prosthecomicrobium and Prosthecochloris; hyphomicrobia (< 1 to 15%), including Hyphomicrobium and Rhodomicrobium; and Ancalomicrobium (< 1 to 6%). The viable counts of heterotrophs also indicated that the caulobacters were the most numerous prosthecate bacteria in the stream. They ranged from fewer than 1 per ml to a maximum of almost 4,000 per ml. During the coldest period, when the total viable counts decreased to about 10(4) per ml compared to their summer high of over 10(7) per ml, the caulobacters actually increased in numbers. In December (temperature 0 to 1 C), they comprised from 0.09 to 1.0% of the viable microbial count, and in March (6.0 to 8.0 C) they accounted for 0.14 to 2.8%. The other heterotrophic prosthecate bacteria were generally found at numbers less than 1 per ml, with the exception of the December study when Hyphomicrobium was present in numbers as high as 2,400 per ml. There was no consistent correlation between the frequency of prosthecate bacteria and total coliforms in the stream during the investigation.

Bacteria↗
PubMed1971

Gas-vacuolated strains of Microcyclus aquaticus.

Two strains of gas-vacuolated heterotrophic bacteria have been isolated from freshwaters by using 0.01% peptone enrichments. Both are nonmotile, vibrioid organisms that form rings before division. In terms of these morphological traits and other characteristics, including nutritional characters and deoxyribonucleic acid base composition, these new isolates are very similar to Microcyclus aquaticus. It is proposed that they be considered strains of this species.

Anaerobiosis↗
PubMed1968

Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria.

Direct microscopic examination of natural freshwater samples reveals a variety of small microorganisms having elaborate cellular appendages. Several strains have been isolated from crude cultures containing low concentrations of organic nutrients. All of the isolates are procaryotic. They are aerobic chemoorganotrophs that require vitamins for growth. Because they cannot be assigned to any of the existing bacterial genera, two new genera are proposed: Ancalomicrobium for organisms which have several long appendages and which reproduce by budding; Prosthecomicrobium for organisms which have many short appendages tapering toward a blunt tip and which reproduce by binary fission. Gas vacuoles have been found in strains of each genus. The term prostheca is proposed for the rigid appendages of procaryotic cells bounded by the cell wall, and is defined to include the structures on these new bacteria, as well as the stalks of the caulobacters and the hyphae of the hyphomicrobia.

Bacteria↗