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Biomedical subjects

J Tagami

Publications and source records attributed to J Tagami.

98 records · Page 6Linked to original sources

In vitro surface analysis of active and arrested dentinal caries using a pH-imaging microscope.

This in vitro study evaluated the pH value of active and arrested caries lesions in deep dentinal caries using a pH-imaging microscope (SCHEM-100, HORIBA Ltd, Kyoto, Japan). Buccal-lingual cut sections of extracted human teeth that had either active or arrested dentinal caries lesions were placed on the pH-imaging sensor of the microscope. The pH values were compared statistically by one-way ANOVA and Fisher's PLSD test (p<0.05). In addition, both types of caries lesions were compared with a caries detector solution for this pH-imaging characterization. For both active and arrested lesions in dentin, the lowest pH values in the caries lesions (range from 5.3 to 6.6) were lower than that of intact dentin (range from 6.8 to 7.4). There were statistical differences between the lowest pH value within the active lesion (range from 5.3 to 5.8) and the arrested lesion (range from 6.3 to 6.6) (p<0.05). Although the arrested lesion was unstainable and impermeable to the dye, there was a close relationship between dye staining and pH-imaging characterization within the active lesion from visual inspection.

Adult↗

Effect of self-etching primer vs phosphoric acid etchant on bonding to bur-prepared dentin.

This study evaluated the effect of dentin conditioner on tensile bond strength to dentin prepared with different types of burs. A self-etching primer system, Mac-Bond II (MB, Tokuyama Dental) and a phosphoric acid etching system, Single Bond (SB, 3M) were used for conditioning. Twenty-four extracted intact human molars were ground flat to expose occlusal dentin. After the dentin surfaces were polished with #600 SiC paper, the teeth were randomly divided into a control group and three experimental groups according to the bur grits used: #600 SiC paper only as the control, fine cut steel bur (SB600), crosscut steel bur (SB703) and regular grit diamond bur (DB) mounted in a dental handpiece utilizing water cooling. The dentin surfaces were treated with one of two adhesive systems, then composite buildups were done with Clearfil AP-X (Kuraray Medical). After soaking the bond specimens for 24 hours in 37 degrees C water, multiple vertical serial sections (0.7 mm thick, 7-8 slices per one tooth) were made, trimmed to form an hour-glass shape with a 1.0 mm2 cross-section and tensile bond strengths were determined at a crosshead speed of 1 mm/minute. Statistical analysis was made using one and two-way ANOVA and Fisher's PLSD test (p<0.05). Six additional molars were used for SEM observations of the dentin surfaces of each group before and after treatment with the self-etching primer of MB, and another four teeth were used to observe the resin-dentin interface of each group of SB. Using MB, the DB group produced the lowest tensile bond strength (TBS) among the groups that received bur preparation, and there were no statistical differences among SB600, SB703 and the control. For SB, the TBS of SB703 was the highest, and there were no statistical differences among the other groups and the control. The influence of the method used to prepare dentin for micro-tensile bond strength testing was dependent on the adhesive system used.

Acid Etching, Dental↗

Shear bond stability of current adhesive systems to enamel.

This study evaluated the micro-shear bond strength of two commercially available resin bonding systems (Single Bond, 3M, USA and SE Bond, Kuraray, Japan) to human enamel. One hundred and twenty enamel sections were prepared from extracted non-carious human molars by cutting with a slowly rotating blade. The enamel surfaces were polished with #280-grift SiC paper under running water. These surfaces were randomly divided into two groups and treated with either Single Bond or Clearfil SE Bond according to manufacturers' instructions. After the bonding procedures, a micro tygon tubing with an internal diameter approximately 0.7 mm and 0.5 mm in height was placed on the enamel surface and Clearfil AP-X resin filled the tube. After the resin was photo-irradiated, the tygon tube was removed. The specimens were further divided into 12 groups according to storage time. All specimens were stored in isotonic sodium chloride solution at 37 degrees C. The bond strength was then measured by means of microshear bond testing at one day, one week, one month, three months, six months and one year. After testing, the fractured surfaces and interfaces were observed using scanning electron microscopy (SEM). The data was analyzed by two-way ANOVA and Fisher's PLSD tests at 95% level of confidence. The result was that both factors (storage time and material) affected bond strength and there was a statistically significant interaction between them. In general, bond strength decreased with time for both materials. The highest bond strengths were achieved at one day with SE Bond and Single Bond. For Single Bond, from one month to one year, the bond strength dramatically decreased. For SE Bond, the decrease in bond strength was gradual. After one-year storage, the bond strength drastically decreased and this value was not significantly different from the results of the one-year storage of Single Bond.

Analysis of Variance↗

Effective bond strength of current adhesive systems on deciduous and permanent dentin.

PURPOSE: To evaluate the bond strength of a total-etching, self-priming system (Single Bond) and a self-etching system (Clearfil SE Bond) to deciduous and permanent human dentin. METHODS AND MATERIALS: Buccal dentin discs were prepared with a diamond disc from permanent first premolars, permanent third molars and deciduous second molars. The flat dentin surfaces were obtained by polishing with wet 600 grit silicon carbide papers. The specimens of each group were further divided into two groups for bonding to either Single Bond or Clearfil SE Bond. After 24 hours, the microshear bond strength testing was executed on a universal testing machine. Statistical analysis was performed at alpha = 0.05. RESULTS: No significant differences in bond strength were found between materials. However, deciduous dentin demonstrated significantly lower bond strengths than permanent premolar dentin when Single Bond was applied (p < 0.05). CONCLUSION: The difference in bonding substrate (permanent or deciduous dentin) had a significant effect on bond strength when the total-etching, self-priming system was applied.

Acid Etching, Dental↗

Early bonding of resin cements to dentin--effect of bonding environment.

The aim of this study was to evaluate early tensile bond strengths of three commercial resin cements (a dual-cured and two chemically cured) to bovine dentin. Bonding was performed in two environmental conditions, namely room environment (23 degrees C/50% RH) and oral environment (30 degrees C/80% RH). Tensile bond strengths were recorded at 10 minutes, 1 hour, and 1 day after the bonding procedure was completed and were analyzed using one-way ANOVA, Fisher's PLSD test, and Student's t-test. The results showed that bond strengths were statistically greater for all materials (P < 0.05) over time, except for Bistite Resin Cement between 10 minutes and 1 hour (P > 0.05). Variation between the bonding environments was observed only for Bistite Resin Cement at both 10 minutes and 1 hour, and Panavia 21 at 10 minutes. It was concluded that bond strengths were initially weak for the chemically cured materials, and all materials showed significantly greater bond strengths over the first 24 hours, but bonding environment had little influence.

Analysis of Variance↗

Monkey pulpal responses to conventional and adhesive luting cements.

Monkey pulpal responses to metal inlays luted with a combination of an adhesive resin and luting composite and conventional dental cements were histopathologically evaluated. Initial pulpal responses caused by re-exposure of the cut dentin surfaces and luting procedure under hydraulic pressure subsided at 90 days after final cementation. There was no significant difference among pulpal reactions to conventional dental cements and a combination of an adhesive resin and luting composite. The adhesive resin coating of freshly cut dentinal walls/floors immediately after cavity preparation seems to provide protection for the dentin and pulp in indirect restorations requiring temporary sealing.

Analysis of Variance↗

Short-term reaction of exposed monkey pulp beneath adhesive resins.

Monkey pulpal responses to four adhesive resin systems used as direct pulp capping agents were histopathologically evaluated at 3, 7, 14, 30, and 60 days after operation. No serious inflammatory reaction of the pulp, such as necrosis or abscess formation, was observed. Slight inflammatory cell infiltration was the main inflammatory reaction of the exposed pulp, and the exposed area became occluded with dentin bridging as the observation period increased. Healing of exposed dental pulp beneath adhesive resin capping slightly differed depending on the materials used.

Analysis of Variance↗

Selective caries removal with air abrasion.

The purpose of this study was to evaluate selective caries removal using an air-abrasive technique. Alumina powders, glass beads, crushed glass powders, and crushed powders of polycarbonate resin were applied to intact human enamel, dentin, and artificially demineralized dentin (caries-model dentin). Furthermore, the effect of the particle size of abrasives and air pressures on the abraded depths was examined. When alumina powders and glass beads were used, the abraded depths of enamel, dentin, and caries model increased as the particle size and air pressure increased. Alumina powders and crushed glass powders abraded intact enamel and dentin more than the caries-model dentin, whereas glass beads abraded the caries-model dentin more than the intact enamel and dentin. Only crushed powders of polycarbonate resin abraded the caries-model dentin without reducing intact enamel and dentin. With hard particles, such as alumina powders, glass beads, or crushed glass powders, selective caries removal by the air-abrasive technique appeared to be difficult to achieve, even if the particle size and the air pressure were changed. Crushed powders of polycarbonate resin that reduced only the caries-model dentin are harder than caries-model dentin, but softer than intact enamel and dentin. This study elucidated the possibility of selective carious dentin removal with the air-abrasive technique.

Air↗