In biopsy samples of the lateral part of the quadriceps femoris muscle of 6 obese diabetic male patients and of 11 obese males with a normal glucose tolerance, the activities of 7 enzymes of energy metabolism were estimated: hexokinase, cytoplasmic glycerol-3-phosphate: NAD dehydrogenase, triosephosphate dehydrogenase, lactate dehydrogenase, citrate synthase, malate dehydrogenase and 3-hydroxyacyl-CoA dehydrogenase. The obese diabetic male patients exhibited decreased activities of enzymes of carbohydrate breakdown and cytoplasmic NAD regeneration. Enzymes connected functionally with aerobic metabolism were less affected. The unchanged activity of 3-hydroxyacyl-CoA dehydrogenase points to an increased role of fatty acid catabolism in the muscle.

3-Hydroxyacyl CoA Dehydrogenases↗

PubMed1976

Changes of acid phosphatase activity of fast and slow rat muscles during ontogenetic development.

1. The activity of acid phosphatase (AP) were studied both bio- and histochemically in fast and slow muscles of the rat during postnatal development. 2. Both biochemical and histochemical methods show at birth a similar, high AP activity in both, fast and slow muscles. From 20 days onward AP activity is higher in the slow soleus than in the fast extensor digitorum longus (EDL) muscle. 3. Fibres with high AP activity have also higher activities of oxidative enzymes and of non-specific esterase (NSE). This property is not coupled to degree of ATP-ase activity; fibres with high or low ATP-ase activity reveal similar activities with respect to AP. 4. There is a "two-phase" developmental change in the degree of enzyme activity in both the fast EDL and slow soleus muscles. AP activity is high at birth, decreases during later postnatal development and increases again in senescent muscles.

Acid Phosphatase↗

PubMed1976

Enzyme activity patterns of energy supplying metabolism in the quadriceps femoris muscle (vastus lateralis): sedentary men and physically active men of different performance levels.

1. In 3 groups of men, differing as to the amount and intensity of physical training loads, increasing in the order "sedentary": "sporting": "athletic", enzyme activities were estimated in biopsy samples of m. quadriceps femoris (vastus lateralis). The enzymes were: Hexokinase (HK), NAD: glycerol-3-phosphate dehydrogenase (GPDH), triosephosphate dehydrogenase (TPDH), lactate dehydrogenase (LDH), citrate synthase (CS), NAD: malate dehydrogenase (MDH), and 3-hydroxyacyl-CoA dehydrogenase (HOADH). Indicators of laboratory performance and whole-body metabolic capacities (maximal oxygen consumption etc.) were estimated in the "sporting" and "athletic" groups. 2. In the 2 latter groups, distinguished by greater physical activity, the atypical enzyme activity pattern, remarkable by a low activity of LDH and high relative activities of GPDH and HK, as reported earlier in a sedentary group (Bass et al., 1975a), disappeared. The possibility of the atypical low LDH enzyme activity pattern as resulting from lack of bodily exertion is discussed. 3. The moderately trained "sporting" group distinguishes itself from the "sedentary" one mainly by a higher activity of LDH and by lower activities of GPDH and MDH. In the intensively trained "athletic" group, enzymes connected to aerobic oxidation (MDH, CS, HOADH) and GPDH also show higher activities than in the "sporting" group. The difference between the two more active groups is further borne out by a higher maximum oxygen uptake and carbon dioxide release of the well-trained "athletic" group. This difference of enzyme activity pattern may not be confined to the quadriceps femoris muscle.

Adult↗

PubMed1974