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J Telch

Publications and source records attributed to J Telch.

5 recordsLinked to original sources
PubMed1992

Reexamination of protein requirements in adult male humans by end-product measurements of leucine and lysine metabolism.

We examined leucine and lysine metabolism in adult humans (n = 8 and n = 5, respectively) to assess the current estimate of protein requirement. Each subject consumed a controlled isoenergetic liquid diet for three 10-d periods at three intakes of protein, 0.6, 0.8 and 1.0 g.kg-1.d-1, in random order. Measurements of flux and catabolism were determined in each subject in both the fed and postabsorptive states, with a primed, 4-h continuous infusion of L-[1-13C]leucine and L-[alpha-15N]lysine. Fluxes of leucine and lysine were not affected by feeding state or protein intake. Adaptation to different protein intakes did not affect the oxidation of leucine and lysine in the postabsorptive state, but leucine oxidation was significantly higher during the fed period compared with the postabsorptive period at a protein intake of 1.0 g.kg-1.d-1 (P less than 0.05) and tended to be greater (P = 0.06) at an intake of 0.8 g.kg-1.d-1. Nitrogen balance assessed over the last 72 h of each dietary period was positive for all subjects at protein intakes of 0.8 and 1.0 g.kg-1.d-1 of protein and, hence, was consistent with the leucine oxidation data. These data suggest that experiments conducted during the postabsorptive period are not appropriate for estimating protein requirements. Leucine oxidation data obtained during the fed state estimate a protein requirement for young men of between 0.6 and 0.8 g.kg-1.d-1.

Adult↗
PubMed1981

Intestinal glucose transport in acute viral enteritis in piglets.

1. We studied intestinal glucose transport in pigs during the acute and convalescent phases of an invasive viral enteritis, transmissible gastroenteritis. 2. When diarhoea was severe 40 h after experimental infection, net absorption of glucose, Na+ and water, measured by marker perfusion in the jejunum, was reduced; the enhancement of Na+ and water absorption in response to increasing perfusate glucose concentrations up to 120 mmol/l was diminished compared with the response observed in control and convalescent pigs. 3. Measured in vitro, 40 h after infection, unidirectional fluxes of 3-O-methyl-D-glucose across the jejunal epithelium were reduced and net absorption of the sugar was obliterated. Phlorizin (0.05 mmol/l), which completely inhibited net 3-O-methyl-D-glucose absorption in control tissue, had no significant effect on transmissible gastroenteritis jejunum. 4. Our data suggest that in this invasive viral enteritis, which closely resembles human rotavirus enteritis, glucose absorption is impaired as a result of defects in both active and passive glucose flux. 5. Differences between the mechanisms of viral diarrhoea, demonstrated by our study and those of the enterotoxigenic diarrhoeas, should be taken into consideration in formulating active therapeutic measures for children with acute viral diarrhoea.

3-O-Methylglucose↗