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Biomedical subjects

J Thoen

Publications and source records attributed to J Thoen.

At least 37 records · Page 2Linked to original sources
PubMed2001

Serglycin expression in CD2+ and CD14+ cells from patients with various rheumatic diseases.

The objective of the study was to look at the in vivo expression of serglycin in cells taken from patients with an inflammatory disease. The mRNA expression of the small proteoglycan serglycin was investigated in macrophages/monocytes and T-cells derived from the synovial fluid and blood of six patients with various rheumatic diseases and from the blood of two control subjects. Our results demonstrate higher Levels of expression in CD14+ cetts taken from patients with chronic inflammatory diseases than in control subjects. This suggests that serglycin may play a role during the inflammatory process.

Adult↗
PubMed2001

Parametric study of the peak negative acoustic pressure distribution within the image plane of a phased array transducer.

A study is presented in which the influence of pulse and transducer characteristics on the distribution of the peak negative acoustic pressure within the image plane of a phased array transducer is shown. First, the influence of the center frequency, the bandwidth of the transmitted pulse, the position of the focus point, and the properties of the phased array are investigated by computer simulation. These simulations show that both the maximal amplitude and the homogeneity of the distribution of the peak negative pressure within a sector image depend on these parameters. Most remarkably, they show that the maximal peak pressure does not necessarily occur on the geometrical symmetry axis of the array transducer but can occur at the outermost lateral lines of a sector image. Second, this effect is demonstrated experimentally by hydrophone measurements of the sound field produced by a standard, clinically used, 2.5-MHz phased array transducer. The experimental results agree well with the theoretical model. The relevance of this study for contrast echography and the safety measurements of ultrasound is discussed.

Acoustics↗
PubMed2001

On the character of acoustic waves at the interface between hard and soft solids and liquids.

Laser ultrasonics is used to optically excite and detect acoustic waves at the interface between a liquid and a solid or coated solid. Several case studies show that this technique is feasible to investigate experimentally the theoretically predicted fundamental properties of different aspects of interface waves at liquid-solid interfaces and to characterize the elastic properties of soft solids. The theoretical prediction that the leaky Rayleigh (LR)-type root of the characteristic determinant becomes forbidden when the shear velocity of the solid lies below the bulk velocity of the liquid was experimentally confirmed. The depth profiling and nondestructive testing potential of Scholte waves was experimentally illustrated and explained by the properties of the wave displacement profile.

Journal Article↗
PubMed2000

High frame rate myocardial integrated backscatter. Does this change our understanding of this acoustic parameter?

AIMS: Integrated backscatter (IB) and its cyclic variation (CV) derived from radio-frequency (RF) data have been used as parameters to attempt myocardial tissue characterization. Prior imaging systems used to measure IB and its CV typically acquired data at frame rates of 20-30 Hz and at a resolution of 6-8 bits. If changes in IB levels are in part related to specific short-lived events, occurring within the cardiac cycle, this frame rate and resolution could have been too low to resolve adequately what might be a more complex data set. METHODS AND RESULTS: To investigate this possibility, we acquired real time two-dimensional (2D) myocardial IQ data (the 'in-phase quadrature' sampled RF data) at high frame rate (> 100 Hz), high dynamic resolution (theoretical 19-bit) and a sector angle of 20 degrees. Several consecutive heart cycles of myocardial data were acquired from individual cardiac walls in five closed chest dogs and 10 healthy, young volunteers at normal heart rates. On the reconstructed RF data regions of interest were indicated, and IB and its CV were calculated. The extracted high frame rate curves showed that the CV of IB is not a smooth sinusoidal-like curve, but is made up of multiple reproducible peaks and troughs with local minima and maxima which are temporally related to active or passive mechanical events, i.e. systolic contraction, early ventricular relaxation and ventricular filling due to atrial contraction. CONCLUSIONS: This study shows that increasing the rate of real-time RF data acquisition results in a more complex, reproducible IB curve. The resolved maxima and minima in IB levels are related to specific phases of the myocardial contraction. Furthermore, spectral analysis showed that IB curves acquired at normal heart rates contain information up to 40 Hz. Hence, cardiac imaging data sets used to analyse regional myocardial function obtained at frequencies lower than 80 frames per second can contain aliased information.

Adult↗
PubMed2000

Changes in the Th1 or Th2 cytokine dominance in the synovium of rheumatoid arthritis (RA): a kinetic study of the Th subsets in one unusual RA patient.

OBJECTIVE: To perform a kinetic study of the Th1/Th2 balance in the rheumatoid arthritis (RA) synovium. METHODS: Three different synovial tissue (ST) samples were obtained from one patient with erosive RA. The characterization of Th1 and Th2 responses was performed by interferon-gamma and interleukin-4 measurements and by expression of the chemokine receptors CCR5 and CCR3. Measurements of secreted and surface immunoglobulin determined the types of B cells. RESULTS: The first ST sample yielded 31 CD4+ T cell clones which showed an unusual Th2 dominant pattern in the inflamed synovium. The Th2 response was associated with predominantly synovial IgG B cells, and a predominantly Th1 profile in the peripheral blood. In contrast, ST samples obtained 2 and 2.5 yr later displayed first a Th0 and thereafter a Th1 profile, and the synovial B cell response was predominantly of IgM type. The T cell lines from the Th1/Th0 tissues expressed the Th1 marker CCR5 but not CCR3, while the T cells from the Th2 tissue expressed the Th2 marker CCR3 and no CCR5. CONCLUSION: These results demonstrate that a predominantly Th2 response can be associated with active erosive RA. However, the Th2 profile was not permanent and changed into a Th0 and thereafter a Th1 profile.

Aged↗
PubMed2000

Phase behavior and blue-phase-III-isotropic critical point in (R)-(S) mixtures of a chiral liquid crystal with a direct twist-grain-boundary to blue-phase transition

We have investigated mixtures of the (R) and (S) enantiomers of a chiral liquid crystal, (R)- or (S)-1-methylheptyl 3'-fluoro-4'-(3-fluoro-4-octadecyloxybenzoyloxy)tolane-4-carboxylate using high-resolution adiabatic scanning calorimetry. The pure (R) compound has a direct transition from the twist-grain-boundary to the blue phase without an intermediary chiral nematic phase. For the blue phases a different kind of phase behavior as a function of enantiomeric excess is observed, most probably related to the presence of a twist-grain-boundary-A instead of a chiral nematic phase below the blue phases. The general form of this phase diagram is compared with traditional blue-phase behavior. Furthermore a blue-phase-III-isotropic phase critical point, analoguous to that of a liquid-gas system, is observed, consistent with experimental and theoretical work recently published in this field. Finally, the effect of changing enantiomeric excess on the latent heats of the different first order phase transitions is measured and discussed.

Journal Article↗
PubMed1999

Decreased CD4+ lymphocyte activation and increased interleukin-4 production in peripheral blood of rheumatoid arthritis patients after acute starvation.

We investigated the effects of acute starvation on mitogen-induced T-cell activation and Th1/Th2 cytokine responses in rheumatoid arthritis (RA) patients. Ten RA patients with active disease underwent a 7-day fast followed by a 2-week refeeding period. Immunological, hormonal, laboratory and clinical evaluations were carried out on days 0, 7 and 21. Using flow cytometry, mitogen-stimulated T-cell activation was assessed in fresh heparinised blood via analysis of CD69 expression. Production of Th1 (interferon-gamma) and Th2 (interleukin-4, IL-4) cytokines was also assessed by ELISA. The 7-day fast significantly decreased the erythrocyte sedimentation rate, C-reactive protein level, joint count, morning stiffness, body weight, CD4+ and CD8+ counts and CD69+ expression on mitogen stimulated CD4+ lymphocytes. A significant increase in mitogen-induced IL-4 production after fasting was found. The fast markedly reduced serum leptin and insulin-like growth factor-1 concentrations. No significant differences occurred in serum cortisol or prolactin before and after fasting. Decreases in CD4+ lymphocyte activation during fasting correlated with decreases in body weight. Our results suggest that the clinical and laboratory improvements in fasting RA patients may be attributed to decreased CD4+ T-cell activation and an increase in the number and/or function of IL-4-producing Th2 cells. Factors associated with loss of body weight during acute starvation appear to have an inhibitory effect on CD4+ lymphocyte activation.

Adult↗
PubMed1999

Glass transition temperature of honey as a function of water content as determined by differential scanning calorimetry.

The glass transition of pure and diluted honey and the glass transition of the maximally freeze-concentrated solution of honey were investigated by differential scanning calorimetry (DSC). The glass transition temperature, of the pure honey samples accepted as unadulterated varied between -42 and -51 degrees C. Dilution of honey to 90 wt % honey content resulted in a shift of the glass transition temperature by -13 to -20 degrees C. The concentration of the maximally freeze-concentrated honey solutions, as expressed in terms of honey content is approximately 102-103%, i.e., slightly more concentrated in sugars than honey itself. The application of DSC measurements of and in characterization of honey may be considered, but requires systematic study on a number of honeys.

Calorimetry, Differential Scanning↗
PubMed1999

Rheumatoid factors in primary Sjögren's syndrome (pSS) use diverse VH region genes, the majority of which show no evidence of somatic hypermutation.

Rheumatoid factor (RF) is the most common autoantibody found in patients with Sjögren's syndrome (SS). To study the genetic origin and the mechanisms acting behind its generation we have characterized and sequenced the immunoglobulin VH genes used by 10 IgM RF MoAbs derived from peripheral blood of six female patients with pSS. We compared the structure of the RF immunoglobulin VH genes with those obtained previously from rheumatoid arthritis (RA) patients and healthy immunized donors (HID). VH1 and VH4 were each used by four RF clones, one clone was encoded by VH3 family gene and one by VH2 family gene. This distribution frequency was different from that observed in RA, where VH3 was the dominant family, followed by VH1. Eight different germ-line (GL) genes encoded the clones and all of these genes were seen previously in RA and/or HID RF. Five clones rearranged to JH6, four rearranged to JH4 and one to JH5, in contrast to RF from RA and HID, where JH4 was most frequently used. D segment use and CDR3 structure were diverse. Interestingly, three out of four VH4 clones used the GL gene DP-79 that was seen frequently in RA RF. The degree of somatic mutation in the pSS RF was very much lower than seen in RA and HID RF. All the pSS RF clones except three were in or very close to GL configuration. This indicates that there is little role for somatic hypermutation and a germinal centre reaction in the generation of RF from peripheral blood in pSS.

Adult↗
PubMed1999

Changes in plasma free fatty acid concentrations in rheumatoid arthritis patients during fasting and their effects upon T-lymphocyte proliferation.

OBJECTIVE: To measure whether changes in the concentrations of circulating free fatty acids (FFAs) after a 7 day fast in rheumatoid arthritis (RA) patients would inhibit in vitro T-lymphocyte proliferation. METHODS: The concentration and composition of plasma FFAs were measured in nine RA patients at the conclusion of a 7 day fast. A FFA mixture was made up based on these findings (20% linoleic, 43% oleic, 10% stearic, 27% palmitic acid). Mitogen-induced lymphocyte proliferative responses were measured after co-culture of peripheral blood mononuclear cells (PBMC) from healthy individuals in the presence of increasing concentrations of this FFA mixture (from 0 to 2000 microM) and in the presence of FFA mixtures where the relative proportions of fatty acids varied. RESULTS: Both the concentration of the FFA mixture and the ratio between the unsaturated and saturated fatty acids significantly influenced in vitro lymphocyte proliferation (P<0.0001). Unexpectedly, the highest concentrations of the FFA mixture increased lymphocyte proliferation. At equimolar concentrations (600 microM), manipulating the amounts of oleic and linoleic fatty acids relative to stearic and palmitic fatty acids had a potent inhibitory effect upon lymphocyte proliferation. CONCLUSION: Fasting-associated increases in total plasma FFA concentrations do not inhibit, but rather enhance, in vitro lymphocyte proliferation. An inhibitory effect could only be achieved by manipulating the balance between the unsaturated and saturated fatty acids.

Adult↗
PubMed1999

Nonlinear propagation effects on broadband attenuation measurements and its implications for ultrasonic tissue characterization.

A study is presented in which the influence of the pressure amplitude of the incident pulse on the estimated frequency dependency of the attenuation coefficient is shown. First, the effect is demonstrated with a simple theoretical model for both transmission and reflection measurements. Simulations show that for both measurement techniques a high-amplitude incident pulse results in a biased estimate of the attenuation coefficient due to nonlinear interaction of the different frequency components of the incident pulse. It is shown that in transmission and reflection measurements the biases have opposite signs. The effect of bandwidth, central frequency, and phase of the incident pulse on this bias is investigated. Second, the effect is demonstrated both in vitro, using a broadband through-transmission substitution technique on a tissue mimicking gelatine phantom, and in vivo, using reflection measurements with standard clinical equipment. The experimental results agree well with the theoretical model. The relevance of this study for ultrasonic tissue characterization is shown.

Culture Techniques↗
PubMed1997

Ultrasonic wave propagation in porous media: determination of acoustic parameters and high frequency limit of the classical models.

Results on the ultrasonic wave propagation in porous materials are presented with emphasis on the measurement of acoustic parameters and on the discrepancy between experimental results and theoretical predictions for the attenuation at high frequencies. This discrepancy can be observed in Biomedical Engineering where the propagation in different sorts of bones is studied as well as in the fields of Geophysics and Material Science. In the present study, the slow wave propagation in polyurethane foams saturated by different gases is investigated in a frequency range of [70-800 kHz]. Methods are presented to determine the tortuosity and the viscous and thermal characteristic lengths. The experimental results, obtained using standard ultrasonic and vacuum equipments, show that an excess attenuation occurs when the wavelength is not sufficiently large compared to the lateral dimensions of the fibers. This effect constitutes a limit of the classical models of equivalent phases. It is evaluated with the help of a model of ultrasonic scattering. A numerical simulation of osteoporosis using Biot's model is also presented.

Acoustics↗
PubMed1993

[Quality of an outpatient rheumatology clinic. Consumers' evaluation of medical aspects and service].

As part of our quality assurance programme we designed special questionnaires which addressed expectations concerning medical aspects and service at our out-patient rheumatology clinic, and the degree of satisfaction. These questionnaires were answered by 97 patients, 86 referring general practitioners and five rheumatologists. The principle purpose of the consultation with the specialist, both for patients and referring practitioners, was to obtain or confirm the diagnosis. 76% of the patients and 91% of the general practitioners were satisfied with the medical aspect of the consultation. The dissatisfied patients were mainly those with symptomatic diagnoses (pain syndromes) not explained by objective changes. As for the service, long waiting time before consultation or before receiving the specialist's report was the main reason for dissatisfaction among patients and referring practitioners respectively.

Adult↗