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Biomedical subjects

J Tippett

Publications and source records attributed to J Tippett.

11 recordsLinked to original sources
PubMed2005

Participatory planning in river catchments, an innovative toolkit tested in Southern Africa and North West England.

The European Union Water Framework Directive (WFD) offers an unparalleled opportunity for improving river basin management. Active participation is essential for its delivery. "End-of-pipe" solutions will not deliver the improvements needed to achieve its ambitious goals. This research tested DesignWays, a toolkit for participatory planning, as a mechanism for maximizing the long-term social and environmental benefits of such stakeholder and community participation. It examined the emerging role of "planning for sustainability" in the context of river catchments. Sustainable management of water requires integration, and recognition of interconnections between systems at different levels of scale. This is an endeavour in which systems thinking provides useful tools. The development of DesignWays was a conscious attempt to embed 'new paradigm' living systems metaphors into a practical planning tool. This paper begins with a description of DesignWays and its development in Southern Africa. An outline of the context of the action research in North-West England is followed by a description of the stages of the process, with highlights of the outcomes. This research had two major outcomes: a contribution to theory through an in-depth exploration of the theoretical basis of participatory, ecologically informed design; and a contribution to practice through investigating DesignWays' potential to meet key challenges of the WFD. This research points to the importance of understanding participatory planning as a societal process, aiming to make the process engaging and meaningful. It has pointed to the need to see participatory planning and education for sustainability as an integrated process. It demonstrated the benefits of an iterative process in which planning at the landscape level of scale informs, and is informed by, work at the site level. It has shown that an approach consistent with a living systems paradigm can contribute to the development of more integrated, ecologically sound plans.

Africa↗
PubMed1997

Biochemical and molecular characterization of the insecticidal fragment of CryV.

Two C-terminal deletion constructs were made to study the effect of such deletions on the biological activity of the CryV protein of Bacillus thuringiensis subsp. kurstaki. The results of feeding on neonatal larvae of Ostrinia nubilalis (European corn borer [ECB]) indicated that the 50% lethal dose of the full-length CryV protein was 3.34 micrograms/g of diet (95% fiducial limits, 2.53 to 4.32 micrograms/g of diet). Removal of 71 amino acids (aa) from the C terminus had little effect on toxicity, whereas deletion of 184 aa abolished the insecticidal activity of the CryV protein completely. Truncations of the full-length CryV protein were also generated with trypsin and the midgut protease of ECB. The proteolytically treated products were characterized by determining their N-terminal amino acid sequences. The CryV protein was found to be cleaved by both proteases through a two-step process. Initially an intermediary form was generated which contained aa 45 of full-length CryV as its N-terminal end. The C-terminal end of this peptide was not experimentally determined. However, analysis of the deduced amino acid sequence of CryV indicated that the C-terminal end of the intermediary form is likely either aa 655 or 659. Further N-terminal processing of the intermediary form resulted in a protease-resistant core form. The core included aa 156 to aa 655 or 659. While the intermediary form retained 100% of the ECB larval toxicity, the core form exhibited only approximately 22% of the toxicity of the full-length protein.

Animals↗
PubMed1994

Providing comfort in the resuscitation room.

Providing comfort in the resuscitation room may not be found high on the list of priorities of nurses working in the Accident and Emergency department. The resuscitation room is an acute care area where patients are very sick and physiological needs are of paramount importance. However, by looking more closely at comfort and what it is, this paper aims to help nurses identify what the components of comfort are and, therefore, recognise their worth and importance when caring for a patient in the resuscitation room.

Communication↗
PubMed1992

Identification and characterization of a novel Bacillus thuringiensis delta-endotoxin entomocidal to coleopteran and lepidopteran larvae.

A new class of Bacillus thuringiensis delta-endotoxins, or insecticidal control proteins (ICPs), is defined by an apparently cryptic protein with a unique primary structure and novel entomocidal specificity for certain coleopteran and lepidopteran species. The discovery of a new group of ICPs will extend the use of this natural insecticide in integrated pest-management systems.

Amino Acid Sequence↗
PubMed1989

Identification and characterization of a serotype b-specific segment of the Haemophilus influenzae genome.

Within the region of the Haemophilus influenzae serotype b genome required for capsule expression, a 4.6-kilobase segment was cloned, used as a hybridization probe, and found to be specific for serotype b strains. Deletion mutagenesis within this segment defined a locus necessary for serotype b capsule production and another involved in maintenance of wild-type colony appearance.

Chromosome Deletion↗
PubMed1986

Mutations affecting expression and maintenance of genes encoding the serotype b capsule of Haemophilus influenzae.

Deletion mutagenesis analysis of a duplicated gene necessary for Haemophilus influenzae serotype b capsule expression showed that only one functional copy of this gene is required for capsule production and for virulence in infant rats. Mutant strains generated in this study differed from each other and from the parental strain in their ability to maintain the large tandem duplication which contains the genes involved in serotype b capsule expression.

Animals↗
PubMed1984

Clinically apparent rubella reinfection.

We report two cases of rubella reinfection in apparently immunocompetent young women. In both cases rubella-specific IgG had been detected on two occasions prior to an illness clinically diagnosed as rubella. Rubella-specific IgM was detected by various techniques in samples of serum collected at the time of the illness.

Adult↗
PubMed1979

A preliminary study of the effect of pyridoxine administration in a subgroup of hyperkinetic children: a double-blind crossover comparison with methylphenidate.

A small sample of six patients with the putative "hyperkinetic syndrome" participated in a research protocol comparing administration of pyridoxine, methylphenidate, and placeboes. The children had had low whole blood serotonin levels and a history of previous responsiveness to methylphenidate. The results of the double-blind clinical evaluation showed trends suggesting that both pyridoxine and methylphenidate were more effective than placebo in suppressing the symptoms of hyperkinesis. Pyridoxine elevated whole-blood serotonin levels, methylphenidate did not. Clinical and laboratory evidence indicated that the pyridoxine effects persisted after the 3-week period when the vitamin had been given in this experimental design.

Adolescent↗
PubMed1975

Developing a utilization review model for community mental health centers.

During their struggles to develop a utilization review model for a community mental health center, members of an interdisciplinary committee identified several issues they believe are important in establishing such a model. They include comprehensiveness of participation and of areas for review (the review committee should represent all disciplines and programs, and should be concerned with any aspect of center functioning), a problem-review approach in which subcommittees carry out documented studies of issues or problems, and specific provision for feedback and implementation of the results.

Community Mental Health Services↗